SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913472_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 12722). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913472_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 2541.143 s (19.017 µs/read; 3.16 M reads/minute). === Summary === Total reads processed: 133,626,654 Reads with adapters: 68,111,581 (51.0%) Reads written (passing filters): 133,626,654 (100.0%) Total basepairs processed: 20,043,998,100 bp Quality-trimmed: 81,027,327 bp (0.4%) Total written (filtered): 19,837,033,305 bp (99.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 68111581 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 52.3% C: 17.2% G: 0.4% T: 30.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 65584896 33406663.5 0 65584896 2 141753 8351665.9 0 141753 3 128632 2087916.5 0 128632 4 89703 521979.1 0 89703 5 79254 130494.8 0 79254 6 61549 32623.7 0 61549 7 65142 8155.9 0 65142 8 69665 2039.0 0 69665 9 55513 509.7 0 55488 25 10 64775 127.4 1 60106 4669 11 62209 31.9 1 56638 5571 12 61277 8.0 1 42220 19057 13 57680 2.0 1 39642 18038 14 62263 2.0 1 43345 18918 15 52380 2.0 1 36283 16097 16 55061 2.0 1 37943 17118 17 65338 2.0 1 45263 20075 18 39363 2.0 1 27457 11906 19 63442 2.0 1 44884 18558 20 47003 2.0 1 32509 14494 21 41379 2.0 1 28904 12475 22 46956 2.0 1 33114 13842 23 45226 2.0 1 32096 13130 24 52332 2.0 1 36942 15390 25 48549 2.0 1 34169 14380 26 36414 2.0 1 25753 10661 27 39614 2.0 1 27943 11671 28 42830 2.0 1 31009 11821 29 36376 2.0 1 25675 10701 30 41030 2.0 1 29617 11413 31 33800 2.0 1 24364 9436 32 37517 2.0 1 27144 10373 33 37385 2.0 1 27250 10135 34 30955 2.0 1 22485 8470 35 29758 2.0 1 21801 7957 36 33182 2.0 1 24246 8936 37 25143 2.0 1 18301 6842 38 31617 2.0 1 23295 8322 39 26690 2.0 1 19546 7144 40 26445 2.0 1 19546 6899 41 20884 2.0 1 15189 5695 42 24002 2.0 1 17830 6172 43 22426 2.0 1 16632 5794 44 23653 2.0 1 17668 5985 45 18780 2.0 1 14044 4736 46 20219 2.0 1 15146 5073 47 19617 2.0 1 14664 4953 48 18929 2.0 1 14106 4823 49 17771 2.0 1 13409 4362 50 16096 2.0 1 11944 4152 51 16117 2.0 1 12071 4046 52 14604 2.0 1 11122 3482 53 14208 2.0 1 10628 3580 54 13894 2.0 1 10500 3394 55 12508 2.0 1 9645 2863 56 13563 2.0 1 10479 3084 57 10196 2.0 1 7741 2455 58 11036 2.0 1 8384 2652 59 12788 2.0 1 9924 2864 60 8114 2.0 1 6080 2034 61 9087 2.0 1 6906 2181 62 9831 2.0 1 7626 2205 63 7809 2.0 1 5965 1844 64 7221 2.0 1 5588 1633 65 12893 2.0 1 10052 2841 66 5205 2.0 1 3910 1295 67 4440 2.0 1 3363 1077 68 4676 2.0 1 3618 1058 69 6175 2.0 1 4767 1408 70 5922 2.0 1 4469 1453 71 4270 2.0 1 3260 1010 72 4785 2.0 1 3667 1118 73 4481 2.0 1 3425 1056 74 4045 2.0 1 3054 991 75 5612 2.0 1 4208 1404 76 3332 2.0 1 2484 848 77 2269 2.0 1 1668 601 78 1040 2.0 1 777 263 79 1464 2.0 1 1128 336 80 1813 2.0 1 1438 375 81 1614 2.0 1 1239 375 82 1692 2.0 1 1283 409 83 1570 2.0 1 1162 408 84 1409 2.0 1 1027 382 85 1275 2.0 1 912 363 86 1313 2.0 1 939 374 87 1093 2.0 1 788 305 88 999 2.0 1 669 330 89 951 2.0 1 655 296 90 888 2.0 1 602 286 91 801 2.0 1 532 269 92 748 2.0 1 451 297 93 665 2.0 1 405 260 94 584 2.0 1 365 219 95 593 2.0 1 335 258 96 531 2.0 1 319 212 97 516 2.0 1 316 200 98 468 2.0 1 275 193 99 457 2.0 1 249 208 100 433 2.0 1 237 196 101 381 2.0 1 215 166 102 423 2.0 1 228 195 103 387 2.0 1 186 201 104 349 2.0 1 177 172 105 342 2.0 1 186 156 106 272 2.0 1 140 132 107 301 2.0 1 144 157 108 300 2.0 1 159 141 109 284 2.0 1 131 153 110 283 2.0 1 160 123 111 211 2.0 1 117 94 112 241 2.0 1 134 107 113 224 2.0 1 105 119 114 224 2.0 1 125 99 115 209 2.0 1 109 100 116 200 2.0 1 91 109 117 186 2.0 1 95 91 118 198 2.0 1 103 95 119 166 2.0 1 78 88 120 181 2.0 1 112 69 121 155 2.0 1 90 65 122 170 2.0 1 95 75 123 155 2.0 1 83 72 124 145 2.0 1 81 64 125 143 2.0 1 80 63 126 123 2.0 1 73 50 127 105 2.0 1 56 49 128 121 2.0 1 68 53 129 92 2.0 1 50 42 130 79 2.0 1 45 34 131 58 2.0 1 34 24 132 71 2.0 1 51 20 133 39 2.0 1 25 14 134 50 2.0 1 38 12 135 20 2.0 1 9 11 136 24 2.0 1 18 6 137 19 2.0 1 16 3 138 32 2.0 1 25 7 139 14 2.0 1 11 3 140 19 2.0 1 13 6 141 15 2.0 1 6 9 142 12 2.0 1 6 6 143 9 2.0 1 1 8 144 15 2.0 1 9 6 145 11 2.0 1 4 7 146 10 2.0 1 5 5 147 13 2.0 1 3 10 148 8 2.0 1 4 4 149 9 2.0 1 5 4 150 12 2.0 1 3 9 RUN STATISTICS FOR INPUT FILE: ERR13913472_2.fastq.gz ============================================= 133626654 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 133626654 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 25255 (0.02%)