SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913472_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 12722). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913472_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2700.686 s (20.211 µs/read; 2.97 M reads/minute). === Summary === Total reads processed: 133,626,654 Reads with adapters: 53,441,638 (40.0%) Reads written (passing filters): 133,626,654 (100.0%) Total basepairs processed: 20,043,998,100 bp Quality-trimmed: 27,633,326 bp (0.1%) Total written (filtered): 19,894,390,347 bp (99.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 53441638 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.7% C: 0.1% G: 13.3% T: 48.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 41682796 33406663.5 0 41682796 2 6255235 8351665.9 0 6255235 3 2410622 2087916.5 0 2410622 4 994244 521979.1 0 994244 5 75257 130494.8 0 75257 6 54498 32623.7 0 54498 7 57158 8155.9 0 57158 8 59522 2039.0 0 59522 9 59988 509.7 0 58899 1089 10 60441 127.4 1 53545 6896 11 64404 31.9 1 55075 9329 12 60611 8.0 1 53673 6938 13 54684 2.0 1 47607 7077 14 56911 2.0 1 48279 8632 15 54728 2.0 1 46970 7758 16 56397 2.0 1 47137 9260 17 57640 2.0 1 48783 8857 18 52047 2.0 1 45873 6174 19 44991 2.0 1 38917 6074 20 49450 2.0 1 42618 6832 21 47822 2.0 1 41441 6381 22 45560 2.0 1 40104 5456 23 46215 2.0 1 39941 6274 24 46390 2.0 1 39490 6900 25 43426 2.0 1 37995 5431 26 41073 2.0 1 35975 5098 27 41895 2.0 1 36429 5466 28 38454 2.0 1 34112 4342 29 40373 2.0 1 34708 5665 30 39056 2.0 1 34625 4431 31 32903 2.0 1 29206 3697 32 34273 2.0 1 30597 3676 33 33556 2.0 1 29402 4154 34 34587 2.0 1 30512 4075 35 31604 2.0 1 28577 3027 36 28766 2.0 1 25080 3686 37 28779 2.0 1 25501 3278 38 25870 2.0 1 23395 2475 39 28680 2.0 1 25031 3649 40 25807 2.0 1 23068 2739 41 23505 2.0 1 21186 2319 42 23344 2.0 1 21160 2184 43 23275 2.0 1 20865 2410 44 21203 2.0 1 18777 2426 45 25646 2.0 1 21605 4041 46 20898 2.0 1 18090 2808 47 31459 2.0 1 28378 3081 48 12422 2.0 1 10540 1882 49 17089 2.0 1 15426 1663 50 55162 2.0 1 51135 4027 51 16275 2.0 1 14549 1726 52 16018 2.0 1 14444 1574 53 23931 2.0 1 21712 2219 54 10203 2.0 1 9153 1050 55 3189 2.0 1 2864 325 56 18034 2.0 1 16929 1105 57 10907 2.0 1 10214 693 58 4139 2.0 1 3695 444 59 6530 2.0 1 6120 410 60 1605 2.0 1 1346 259 61 7009 2.0 1 6381 628 62 1802 2.0 1 1542 260 63 1055 2.0 1 850 205 64 6426 2.0 1 6000 426 65 3307 2.0 1 3110 197 66 824 2.0 1 685 139 67 2717 2.0 1 2414 303 68 5376 2.0 1 4984 392 69 1802 2.0 1 1603 199 70 4120 2.0 1 3731 389 71 4219 2.0 1 3807 412 72 6672 2.0 1 6152 520 73 1132 2.0 1 946 186 74 384 2.0 1 291 93 75 948 2.0 1 849 99 76 1735 2.0 1 1603 132 77 2186 2.0 1 1978 208 78 2197 2.0 1 1981 216 79 2045 2.0 1 1859 186 80 2046 2.0 1 1825 221 81 1695 2.0 1 1512 183 82 1685 2.0 1 1460 225 83 1476 2.0 1 1326 150 84 1292 2.0 1 1126 166 85 1168 2.0 1 1011 157 86 1138 2.0 1 973 165 87 957 2.0 1 828 129 88 832 2.0 1 702 130 89 821 2.0 1 708 113 90 788 2.0 1 640 148 91 661 2.0 1 541 120 92 550 2.0 1 447 103 93 515 2.0 1 412 103 94 491 2.0 1 388 103 95 427 2.0 1 335 92 96 437 2.0 1 340 97 97 382 2.0 1 288 94 98 320 2.0 1 248 72 99 329 2.0 1 245 84 100 300 2.0 1 208 92 101 273 2.0 1 203 70 102 291 2.0 1 204 87 103 275 2.0 1 204 71 104 244 2.0 1 166 78 105 213 2.0 1 144 69 106 190 2.0 1 132 58 107 187 2.0 1 121 66 108 204 2.0 1 139 65 109 202 2.0 1 133 69 110 210 2.0 1 136 74 111 157 2.0 1 116 41 112 170 2.0 1 123 47 113 147 2.0 1 106 41 114 151 2.0 1 107 44 115 138 2.0 1 90 48 116 143 2.0 1 92 51 117 119 2.0 1 79 40 118 140 2.0 1 94 46 119 111 2.0 1 66 45 120 142 2.0 1 90 52 121 118 2.0 1 82 36 122 120 2.0 1 69 51 123 115 2.0 1 82 33 124 118 2.0 1 79 39 125 109 2.0 1 73 36 126 79 2.0 1 61 18 127 83 2.0 1 60 23 128 89 2.0 1 63 26 129 59 2.0 1 43 16 130 61 2.0 1 39 22 131 48 2.0 1 37 11 132 69 2.0 1 56 13 133 51 2.0 1 31 20 134 42 2.0 1 29 13 135 28 2.0 1 15 13 136 24 2.0 1 18 6 137 23 2.0 1 18 5 138 30 2.0 1 22 8 139 19 2.0 1 14 5 140 25 2.0 1 14 11 141 14 2.0 1 9 5 142 14 2.0 1 7 7 143 9 2.0 1 1 8 144 22 2.0 1 11 11 145 13 2.0 1 4 9 146 26 2.0 1 4 22 147 21 2.0 1 4 17 148 22 2.0 1 3 19 149 57 2.0 1 7 50 150 610 2.0 1 9 601 RUN STATISTICS FOR INPUT FILE: ERR13913472_1.fastq.gz ============================================= 133626654 sequences processed in total