SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913471_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 17156). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913471_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1617.405 s (17.494 µs/read; 3.43 M reads/minute). === Summary === Total reads processed: 92,457,337 Reads with adapters: 37,306,271 (40.3%) Reads written (passing filters): 92,457,337 (100.0%) Total basepairs processed: 13,868,600,550 bp Quality-trimmed: 13,942,160 bp (0.1%) Total written (filtered): 13,756,074,184 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 37306271 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.9% C: 0.1% G: 13.7% T: 48.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 28680032 23114334.2 0 28680032 2 4321587 5778583.6 0 4321587 3 1662323 1444645.9 0 1662323 4 715598 361161.5 0 715598 5 60896 90290.4 0 60896 6 50237 22572.6 0 50237 7 51122 5643.1 0 51122 8 53049 1410.8 0 53049 9 52996 352.7 0 52205 791 10 53110 88.2 1 48216 4894 11 55726 22.0 1 49163 6563 12 53067 5.5 1 48110 4957 13 47512 1.4 1 42606 4906 14 50861 1.4 1 44606 6255 15 47774 1.4 1 42606 5168 16 50039 1.4 1 43341 6698 17 49387 1.4 1 42895 6492 18 45868 1.4 1 41060 4808 19 42677 1.4 1 38349 4328 20 43564 1.4 1 38844 4720 21 43577 1.4 1 38800 4777 22 40684 1.4 1 36849 3835 23 41274 1.4 1 37016 4258 24 41128 1.4 1 36268 4860 25 38116 1.4 1 34380 3736 26 37813 1.4 1 34322 3491 27 38048 1.4 1 34475 3573 28 34433 1.4 1 31413 3020 29 36141 1.4 1 32502 3639 30 34161 1.4 1 31233 2928 31 31641 1.4 1 28922 2719 32 31515 1.4 1 28795 2720 33 31529 1.4 1 28219 3310 34 29236 1.4 1 26861 2375 35 29866 1.4 1 26891 2975 36 29528 1.4 1 26638 2890 37 26879 1.4 1 24495 2384 38 26082 1.4 1 24095 1987 39 24522 1.4 1 22526 1996 40 25080 1.4 1 22956 2124 41 22890 1.4 1 21099 1791 42 23011 1.4 1 20931 2080 43 21109 1.4 1 19564 1545 44 23122 1.4 1 21180 1942 45 20840 1.4 1 18462 2378 46 19768 1.4 1 17970 1798 47 31345 1.4 1 29018 2327 48 10168 1.4 1 9110 1058 49 17333 1.4 1 16055 1278 50 81242 1.4 1 76821 4421 51 19893 1.4 1 18352 1541 52 11605 1.4 1 10738 867 53 23268 1.4 1 21770 1498 54 10756 1.4 1 9926 830 55 2089 1.4 1 1866 223 56 16411 1.4 1 15593 818 57 6976 1.4 1 6560 416 58 2594 1.4 1 2348 246 59 3938 1.4 1 3707 231 60 980 1.4 1 843 137 61 5389 1.4 1 5015 374 62 1423 1.4 1 1251 172 63 880 1.4 1 758 122 64 6095 1.4 1 5762 333 65 2220 1.4 1 2080 140 66 746 1.4 1 647 99 67 2447 1.4 1 2248 199 68 4969 1.4 1 4687 282 69 1559 1.4 1 1402 157 70 4269 1.4 1 4008 261 71 4740 1.4 1 4407 333 72 8310 1.4 1 7803 507 73 3303 1.4 1 3042 261 74 809 1.4 1 696 113 75 598 1.4 1 523 75 76 1552 1.4 1 1453 99 77 2251 1.4 1 2128 123 78 2441 1.4 1 2282 159 79 2407 1.4 1 2278 129 80 2288 1.4 1 2136 152 81 2026 1.4 1 1888 138 82 1830 1.4 1 1698 132 83 1679 1.4 1 1553 126 84 1386 1.4 1 1272 114 85 1386 1.4 1 1294 92 86 1180 1.4 1 1079 101 87 990 1.4 1 910 80 88 958 1.4 1 896 62 89 772 1.4 1 697 75 90 673 1.4 1 607 66 91 602 1.4 1 541 61 92 551 1.4 1 488 63 93 460 1.4 1 409 51 94 457 1.4 1 402 55 95 356 1.4 1 311 45 96 315 1.4 1 287 28 97 288 1.4 1 248 40 98 262 1.4 1 214 48 99 202 1.4 1 171 31 100 200 1.4 1 163 37 101 160 1.4 1 130 30 102 148 1.4 1 126 22 103 137 1.4 1 105 32 104 127 1.4 1 99 28 105 124 1.4 1 90 34 106 115 1.4 1 87 28 107 115 1.4 1 75 40 108 77 1.4 1 52 25 109 93 1.4 1 66 27 110 88 1.4 1 69 19 111 73 1.4 1 53 20 112 79 1.4 1 55 24 113 80 1.4 1 59 21 114 69 1.4 1 55 14 115 82 1.4 1 51 31 116 69 1.4 1 41 28 117 60 1.4 1 45 15 118 50 1.4 1 31 19 119 55 1.4 1 33 22 120 71 1.4 1 51 20 121 51 1.4 1 41 10 122 63 1.4 1 47 16 123 50 1.4 1 32 18 124 53 1.4 1 41 12 125 51 1.4 1 35 16 126 43 1.4 1 29 14 127 50 1.4 1 35 15 128 49 1.4 1 34 15 129 51 1.4 1 40 11 130 49 1.4 1 41 8 131 24 1.4 1 18 6 132 36 1.4 1 27 9 133 27 1.4 1 20 7 134 24 1.4 1 20 4 135 24 1.4 1 16 8 136 20 1.4 1 11 9 137 12 1.4 1 11 1 138 11 1.4 1 6 5 139 9 1.4 1 7 2 140 7 1.4 1 6 1 141 9 1.4 1 6 3 142 14 1.4 1 10 4 143 6 1.4 1 2 4 144 16 1.4 1 7 9 145 18 1.4 1 7 11 146 10 1.4 1 0 10 147 13 1.4 1 4 9 148 20 1.4 1 4 16 149 22 1.4 1 5 17 150 282 1.4 1 1 281 RUN STATISTICS FOR INPUT FILE: ERR13913471_1.fastq.gz ============================================= 92457337 sequences processed in total