SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913470_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 18467). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913470_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1698.340 s (19.182 µs/read; 3.13 M reads/minute). === Summary === Total reads processed: 88,538,712 Reads with adapters: 35,839,571 (40.5%) Reads written (passing filters): 88,538,712 (100.0%) Total basepairs processed: 13,280,806,800 bp Quality-trimmed: 12,037,777 bp (0.1%) Total written (filtered): 13,170,048,855 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 35839571 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.9% C: 0.1% G: 13.6% T: 48.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 27475211 22134678.0 0 27475211 2 4137837 5533669.5 0 4137837 3 1594060 1383417.4 0 1594060 4 688596 345854.3 0 688596 5 61162 86463.6 0 61162 6 50674 21615.9 0 50674 7 50908 5404.0 0 50908 8 52872 1351.0 0 52872 9 53047 337.7 0 52231 816 10 53021 84.4 1 48281 4740 11 55615 21.1 1 49202 6413 12 53307 5.3 1 48505 4802 13 47879 1.3 1 43384 4495 14 50942 1.3 1 44920 6022 15 47539 1.3 1 42782 4757 16 49394 1.3 1 43244 6150 17 49159 1.3 1 43222 5937 18 45654 1.3 1 41294 4360 19 42603 1.3 1 38624 3979 20 43100 1.3 1 38861 4239 21 42907 1.3 1 38601 4306 22 40904 1.3 1 37410 3494 23 41097 1.3 1 37208 3889 24 41470 1.3 1 37074 4396 25 38582 1.3 1 35267 3315 26 37829 1.3 1 34599 3230 27 37666 1.3 1 34461 3205 28 34578 1.3 1 32044 2534 29 35818 1.3 1 32459 3359 30 34201 1.3 1 31553 2648 31 31796 1.3 1 29355 2441 32 31688 1.3 1 29129 2559 33 31656 1.3 1 28662 2994 34 30956 1.3 1 28384 2572 35 29125 1.3 1 26696 2429 36 28933 1.3 1 26808 2125 37 25649 1.3 1 23704 1945 38 25952 1.3 1 24152 1800 39 26236 1.3 1 23978 2258 40 23872 1.3 1 22259 1613 41 24984 1.3 1 22563 2421 42 22951 1.3 1 21285 1666 43 21303 1.3 1 19711 1592 44 21375 1.3 1 19920 1455 45 21842 1.3 1 20011 1831 46 19004 1.3 1 17563 1441 47 28705 1.3 1 26987 1718 48 9477 1.3 1 8606 871 49 15915 1.3 1 15026 889 50 18714 1.3 1 17441 1273 51 14731 1.3 1 13772 959 52 16125 1.3 1 14893 1232 53 17011 1.3 1 15624 1387 54 13024 1.3 1 12234 790 55 10877 1.3 1 10259 618 56 13409 1.3 1 12540 869 57 13897 1.3 1 13084 813 58 10734 1.3 1 9945 789 59 13233 1.3 1 12526 707 60 8764 1.3 1 8201 563 61 10551 1.3 1 9833 718 62 8169 1.3 1 7799 370 63 5721 1.3 1 5329 392 64 9349 1.3 1 8836 513 65 9320 1.3 1 8952 368 66 5081 1.3 1 4760 321 67 5431 1.3 1 5035 396 68 7033 1.3 1 6688 345 69 6078 1.3 1 5721 357 70 7111 1.3 1 6679 432 71 6663 1.3 1 6244 419 72 11603 1.3 1 10973 630 73 1157 1.3 1 998 159 74 523 1.3 1 454 69 75 1831 1.3 1 1724 107 76 2797 1.3 1 2674 123 77 3003 1.3 1 2835 168 78 3044 1.3 1 2866 178 79 2746 1.3 1 2603 143 80 2448 1.3 1 2308 140 81 2305 1.3 1 2160 145 82 2034 1.3 1 1933 101 83 1826 1.3 1 1716 110 84 1622 1.3 1 1509 113 85 1414 1.3 1 1310 104 86 1226 1.3 1 1157 69 87 1052 1.3 1 980 72 88 982 1.3 1 895 87 89 844 1.3 1 777 67 90 786 1.3 1 728 58 91 637 1.3 1 586 51 92 562 1.3 1 504 58 93 469 1.3 1 410 59 94 391 1.3 1 356 35 95 335 1.3 1 295 40 96 286 1.3 1 252 34 97 285 1.3 1 246 39 98 227 1.3 1 190 37 99 183 1.3 1 152 31 100 169 1.3 1 140 29 101 178 1.3 1 143 35 102 143 1.3 1 106 37 103 132 1.3 1 98 34 104 93 1.3 1 64 29 105 97 1.3 1 74 23 106 87 1.3 1 66 21 107 79 1.3 1 57 22 108 107 1.3 1 83 24 109 73 1.3 1 54 19 110 78 1.3 1 51 27 111 69 1.3 1 48 21 112 74 1.3 1 59 15 113 76 1.3 1 50 26 114 63 1.3 1 39 24 115 53 1.3 1 38 15 116 63 1.3 1 44 19 117 61 1.3 1 50 11 118 62 1.3 1 42 20 119 48 1.3 1 36 12 120 64 1.3 1 41 23 121 40 1.3 1 26 14 122 51 1.3 1 36 15 123 45 1.3 1 34 11 124 38 1.3 1 29 9 125 41 1.3 1 28 13 126 50 1.3 1 43 7 127 35 1.3 1 21 14 128 38 1.3 1 27 11 129 34 1.3 1 26 8 130 38 1.3 1 31 7 131 31 1.3 1 24 7 132 24 1.3 1 19 5 133 30 1.3 1 26 4 134 24 1.3 1 20 4 135 16 1.3 1 11 5 136 28 1.3 1 24 4 137 8 1.3 1 7 1 138 17 1.3 1 13 4 139 10 1.3 1 7 3 140 12 1.3 1 12 141 4 1.3 1 4 142 6 1.3 1 1 5 143 6 1.3 1 3 3 144 11 1.3 1 6 5 145 9 1.3 1 4 5 146 11 1.3 1 6 5 147 10 1.3 1 5 5 148 15 1.3 1 5 10 149 23 1.3 1 3 20 150 302 1.3 1 3 299 RUN STATISTICS FOR INPUT FILE: ERR13913470_1.fastq.gz ============================================= 88538712 sequences processed in total