Bismark report for: ERR13913469_val_1.fq.gz and ERR13913469_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 113039746 Number of paired-end alignments with a unique best hit: 59129152 Mapping efficiency: 52.3% Sequence pairs with no alignments under any condition: 44240807 Sequence pairs did not map uniquely: 9669787 Sequence pairs which were discarded because genomic sequence could not be extracted: 29 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 29567657 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 29561466 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2905818943 Total methylated C's in CpG context: 38473321 Total methylated C's in CHG context: 1258909 Total methylated C's in CHH context: 4407924 Total methylated C's in Unknown context: 90540 Total unmethylated C's in CpG context: 306349201 Total unmethylated C's in CHG context: 479217276 Total unmethylated C's in CHH context: 2076112312 Total unmethylated C's in Unknown context: 8312406 C methylated in CpG context: 11.2% C methylated in CHG context: 0.3% C methylated in CHH context: 0.2% C methylated in Unknown context (CN or CHN): 1.1% Bismark completed in 0d 5h 23m 0s