SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913469_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 17848). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913469_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2491.944 s (22.039 µs/read; 2.72 M reads/minute). === Summary === Total reads processed: 113,067,415 Reads with adapters: 45,788,928 (40.5%) Reads written (passing filters): 113,067,415 (100.0%) Total basepairs processed: 16,960,112,250 bp Quality-trimmed: 21,372,451 bp (0.1%) Total written (filtered): 16,813,116,355 bp (99.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 45788928 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.7% C: 0.1% G: 13.6% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 35182676 28266853.8 0 35182676 2 5213765 7066713.4 0 5213765 3 2033904 1766678.4 0 2033904 4 864190 441669.6 0 864190 5 84098 110417.4 0 84098 6 61529 27604.3 0 61529 7 64908 6901.1 0 64908 8 67816 1725.3 0 67816 9 67631 431.3 0 66736 895 10 68393 107.8 1 61034 7359 11 74319 27.0 1 63532 10787 12 68999 6.7 1 61323 7676 13 63379 1.7 1 55637 7742 14 65393 1.7 1 56257 9136 15 62471 1.7 1 54162 8309 16 64444 1.7 1 54652 9792 17 66755 1.7 1 57333 9422 18 60724 1.7 1 54079 6645 19 52467 1.7 1 46066 6401 20 57614 1.7 1 50265 7349 21 55765 1.7 1 48894 6871 22 52940 1.7 1 47023 5917 23 53383 1.7 1 46658 6725 24 53814 1.7 1 45949 7865 25 50684 1.7 1 44561 6123 26 48036 1.7 1 42571 5465 27 49695 1.7 1 43820 5875 28 44720 1.7 1 40101 4619 29 47516 1.7 1 41325 6191 30 45159 1.7 1 40504 4655 31 39204 1.7 1 35171 4033 32 40629 1.7 1 36500 4129 33 40690 1.7 1 36054 4636 34 40679 1.7 1 36196 4483 35 35021 1.7 1 32039 2982 36 39333 1.7 1 35364 3969 37 36320 1.7 1 31779 4541 38 32122 1.7 1 28770 3352 39 31994 1.7 1 29148 2846 40 29212 1.7 1 26368 2844 41 30611 1.7 1 27831 2780 42 28174 1.7 1 25572 2602 43 28013 1.7 1 25099 2914 44 26106 1.7 1 23776 2330 45 27643 1.7 1 24884 2759 46 26349 1.7 1 23201 3148 47 33830 1.7 1 31421 2409 48 13559 1.7 1 11897 1662 49 16625 1.7 1 15535 1090 50 21210 1.7 1 19505 1705 51 18920 1.7 1 17294 1626 52 18446 1.7 1 16672 1774 53 20811 1.7 1 18407 2404 54 17600 1.7 1 16161 1439 55 13542 1.7 1 12640 902 56 14841 1.7 1 13769 1072 57 16625 1.7 1 15336 1289 58 13285 1.7 1 12146 1139 59 14506 1.7 1 13515 991 60 13283 1.7 1 12251 1032 61 11458 1.7 1 10406 1052 62 9986 1.7 1 9422 564 63 7982 1.7 1 7260 722 64 11318 1.7 1 10466 852 65 10242 1.7 1 9713 529 66 7822 1.7 1 7292 530 67 6214 1.7 1 5665 549 68 9696 1.7 1 9089 607 69 8199 1.7 1 7588 611 70 10118 1.7 1 9417 701 71 7376 1.7 1 6751 625 72 10031 1.7 1 9267 764 73 827 1.7 1 660 167 74 1016 1.7 1 896 120 75 2532 1.7 1 2344 188 76 3336 1.7 1 3108 228 77 3610 1.7 1 3368 242 78 3617 1.7 1 3362 255 79 3389 1.7 1 3129 260 80 3122 1.7 1 2862 260 81 2939 1.7 1 2708 231 82 2678 1.7 1 2441 237 83 2425 1.7 1 2255 170 84 2227 1.7 1 2039 188 85 1971 1.7 1 1783 188 86 1806 1.7 1 1649 157 87 1500 1.7 1 1353 147 88 1410 1.7 1 1256 154 89 1266 1.7 1 1123 143 90 1196 1.7 1 1043 153 91 1024 1.7 1 881 143 92 902 1.7 1 795 107 93 768 1.7 1 677 91 94 688 1.7 1 585 103 95 623 1.7 1 531 92 96 536 1.7 1 448 88 97 495 1.7 1 418 77 98 453 1.7 1 372 81 99 399 1.7 1 328 71 100 381 1.7 1 282 99 101 314 1.7 1 250 64 102 278 1.7 1 219 59 103 243 1.7 1 167 76 104 247 1.7 1 176 71 105 234 1.7 1 174 60 106 221 1.7 1 164 57 107 218 1.7 1 149 69 108 181 1.7 1 115 66 109 157 1.7 1 115 42 110 166 1.7 1 117 49 111 135 1.7 1 95 40 112 138 1.7 1 87 51 113 138 1.7 1 85 53 114 128 1.7 1 87 41 115 124 1.7 1 91 33 116 123 1.7 1 80 43 117 104 1.7 1 69 35 118 112 1.7 1 68 44 119 115 1.7 1 79 36 120 93 1.7 1 69 24 121 95 1.7 1 63 32 122 80 1.7 1 51 29 123 80 1.7 1 54 26 124 80 1.7 1 60 20 125 67 1.7 1 45 22 126 95 1.7 1 62 33 127 58 1.7 1 42 16 128 79 1.7 1 51 28 129 61 1.7 1 41 20 130 53 1.7 1 38 15 131 36 1.7 1 27 9 132 38 1.7 1 28 10 133 38 1.7 1 27 11 134 29 1.7 1 15 14 135 30 1.7 1 26 4 136 25 1.7 1 19 6 137 24 1.7 1 10 14 138 10 1.7 1 8 2 139 9 1.7 1 4 5 140 12 1.7 1 9 3 141 17 1.7 1 9 8 142 14 1.7 1 6 8 143 7 1.7 1 3 4 144 11 1.7 1 8 3 145 15 1.7 1 6 9 146 9 1.7 1 2 7 147 10 1.7 1 4 6 148 21 1.7 1 11 10 149 32 1.7 1 3 29 150 376 1.7 1 0 376 RUN STATISTICS FOR INPUT FILE: ERR13913469_1.fastq.gz ============================================= 113067415 sequences processed in total