SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913468_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 28745). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913468_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2188.426 s (20.668 µs/read; 2.90 M reads/minute). === Summary === Total reads processed: 105,884,797 Reads with adapters: 44,001,667 (41.6%) Reads written (passing filters): 105,884,797 (100.0%) Total basepairs processed: 15,882,719,550 bp Quality-trimmed: 21,881,622 bp (0.1%) Total written (filtered): 15,699,370,037 bp (98.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 44001667 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.2% G: 13.6% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 32635237 26471199.2 0 32635237 2 4783505 6617799.8 0 4783505 3 1920269 1654450.0 0 1920269 4 841232 413612.5 0 841232 5 126579 103403.1 0 126579 6 93617 25850.8 0 93617 7 99486 6462.7 0 99486 8 103570 1615.7 0 103570 9 102955 403.9 0 102053 902 10 100746 101.0 1 91379 9367 11 110042 25.2 1 94768 15274 12 101080 6.3 1 90377 10703 13 92880 1.6 1 82050 10830 14 96441 1.6 1 83028 13413 15 93229 1.6 1 81412 11817 16 98479 1.6 1 83867 14612 17 103329 1.6 1 88960 14369 18 92917 1.6 1 83051 9866 19 77445 1.6 1 68423 9022 20 85412 1.6 1 75092 10320 21 81303 1.6 1 71674 9629 22 77015 1.6 1 68681 8334 23 78682 1.6 1 69211 9471 24 78155 1.6 1 67169 10986 25 73055 1.6 1 64991 8064 26 71166 1.6 1 63496 7670 27 73734 1.6 1 65237 8497 28 69962 1.6 1 62802 7160 29 74126 1.6 1 64739 9387 30 69361 1.6 1 62704 6657 31 58237 1.6 1 52594 5643 32 62434 1.6 1 56194 6240 33 59776 1.6 1 52925 6851 34 63683 1.6 1 56707 6976 35 49172 1.6 1 45054 4118 36 54829 1.6 1 49713 5116 37 61033 1.6 1 55126 5907 38 49843 1.6 1 43303 6540 39 51191 1.6 1 46665 4526 40 47312 1.6 1 43286 4026 41 44027 1.6 1 40122 3905 42 45439 1.6 1 40793 4646 43 43320 1.6 1 39523 3797 44 40046 1.6 1 36746 3300 45 44933 1.6 1 40024 4909 46 35209 1.6 1 31832 3377 47 56769 1.6 1 52371 4398 48 18288 1.6 1 16250 2038 49 31920 1.6 1 29550 2370 50 37777 1.6 1 34759 3018 51 29511 1.6 1 27282 2229 52 31540 1.6 1 28587 2953 53 33014 1.6 1 29695 3319 54 27076 1.6 1 25077 1999 55 22887 1.6 1 21371 1516 56 25830 1.6 1 23893 1937 57 29241 1.6 1 27209 2032 58 21355 1.6 1 19706 1649 59 28391 1.6 1 26516 1875 60 22110 1.6 1 20605 1505 61 19381 1.6 1 17774 1607 62 17856 1.6 1 16946 910 63 12433 1.6 1 11547 886 64 19167 1.6 1 17952 1215 65 19128 1.6 1 18185 943 66 12120 1.6 1 11325 795 67 10374 1.6 1 9601 773 68 16202 1.6 1 15263 939 69 13861 1.6 1 13022 839 70 17722 1.6 1 16650 1072 71 13332 1.6 1 12354 978 72 19381 1.6 1 18018 1363 73 1384 1.6 1 1117 267 74 1479 1.6 1 1340 139 75 4297 1.6 1 4086 211 76 5846 1.6 1 5540 306 77 6195 1.6 1 5825 370 78 6249 1.6 1 5881 368 79 5875 1.6 1 5541 334 80 5479 1.6 1 5140 339 81 5001 1.6 1 4714 287 82 4399 1.6 1 4137 262 83 3997 1.6 1 3725 272 84 3395 1.6 1 3172 223 85 3028 1.6 1 2814 214 86 2702 1.6 1 2507 195 87 2401 1.6 1 2218 183 88 2079 1.6 1 1900 179 89 1829 1.6 1 1662 167 90 1635 1.6 1 1498 137 91 1401 1.6 1 1278 123 92 1199 1.6 1 1102 97 93 1038 1.6 1 927 111 94 862 1.6 1 771 91 95 756 1.6 1 677 79 96 630 1.6 1 546 84 97 545 1.6 1 468 77 98 494 1.6 1 425 69 99 428 1.6 1 364 64 100 339 1.6 1 283 56 101 287 1.6 1 235 52 102 262 1.6 1 202 60 103 268 1.6 1 214 54 104 213 1.6 1 160 53 105 182 1.6 1 139 43 106 156 1.6 1 123 33 107 176 1.6 1 124 52 108 170 1.6 1 119 51 109 135 1.6 1 85 50 110 140 1.6 1 96 44 111 130 1.6 1 87 43 112 102 1.6 1 72 30 113 115 1.6 1 74 41 114 101 1.6 1 78 23 115 111 1.6 1 78 33 116 105 1.6 1 73 32 117 86 1.6 1 65 21 118 128 1.6 1 81 47 119 94 1.6 1 66 28 120 98 1.6 1 68 30 121 73 1.6 1 44 29 122 98 1.6 1 79 19 123 77 1.6 1 56 21 124 89 1.6 1 75 14 125 69 1.6 1 54 15 126 83 1.6 1 53 30 127 64 1.6 1 51 13 128 65 1.6 1 47 18 129 57 1.6 1 35 22 130 48 1.6 1 36 12 131 49 1.6 1 35 14 132 46 1.6 1 42 4 133 33 1.6 1 24 9 134 28 1.6 1 23 5 135 28 1.6 1 23 5 136 28 1.6 1 20 8 137 33 1.6 1 25 8 138 26 1.6 1 22 4 139 20 1.6 1 13 7 140 27 1.6 1 17 10 141 15 1.6 1 8 7 142 12 1.6 1 9 3 143 9 1.6 1 5 4 144 8 1.6 1 4 4 145 13 1.6 1 7 6 146 13 1.6 1 5 8 147 13 1.6 1 5 8 148 15 1.6 1 2 13 149 29 1.6 1 3 26 150 319 1.6 1 11 308 RUN STATISTICS FOR INPUT FILE: ERR13913468_1.fastq.gz ============================================= 105884797 sequences processed in total