SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913467_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 11731). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913467_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2181.390 s (22.241 µs/read; 2.70 M reads/minute). === Summary === Total reads processed: 98,078,887 Reads with adapters: 39,200,096 (40.0%) Reads written (passing filters): 98,078,887 (100.0%) Total basepairs processed: 14,711,833,050 bp Quality-trimmed: 21,162,227 bp (0.1%) Total written (filtered): 14,603,776,638 bp (99.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 39200096 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.8% C: 0.1% G: 13.4% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 30676561 24519721.8 0 30676561 2 4590380 6129930.4 0 4590380 3 1766986 1532482.6 0 1766986 4 726555 383120.7 0 726555 5 51242 95780.2 0 51242 6 36315 23945.0 0 36315 7 38310 5986.3 0 38310 8 40041 1496.6 0 40041 9 39999 374.1 0 39214 785 10 40824 93.5 1 36007 4817 11 43988 23.4 1 37390 6598 12 40903 5.8 1 36107 4796 13 36564 1.5 1 31865 4699 14 38673 1.5 1 33058 5615 15 36601 1.5 1 31410 5191 16 38235 1.5 1 32119 6116 17 39091 1.5 1 33212 5879 18 35386 1.5 1 31281 4105 19 30126 1.5 1 26180 3946 20 33947 1.5 1 29297 4650 21 32137 1.5 1 27908 4229 22 30639 1.5 1 27015 3624 23 31274 1.5 1 27247 4027 24 31666 1.5 1 26856 4810 25 29385 1.5 1 25694 3691 26 28094 1.5 1 24720 3374 27 28503 1.5 1 24865 3638 28 26019 1.5 1 23193 2826 29 27728 1.5 1 23822 3906 30 26460 1.5 1 23535 2925 31 22227 1.5 1 19829 2398 32 23410 1.5 1 20931 2479 33 23278 1.5 1 20472 2806 34 22123 1.5 1 19782 2341 35 22033 1.5 1 19368 2665 36 21975 1.5 1 19368 2607 37 22740 1.5 1 20129 2611 38 19531 1.5 1 17567 1964 39 15326 1.5 1 13841 1485 40 17903 1.5 1 16144 1759 41 17480 1.5 1 15856 1624 42 16838 1.5 1 14752 2086 43 14369 1.5 1 13157 1212 44 16754 1.5 1 14916 1838 45 16958 1.5 1 14128 2830 46 16906 1.5 1 14264 2642 47 23797 1.5 1 21399 2398 48 10023 1.5 1 8292 1731 49 13679 1.5 1 12127 1552 50 71538 1.5 1 67078 4460 51 37063 1.5 1 34718 2345 52 3190 1.5 1 2668 522 53 7956 1.5 1 7162 794 54 6639 1.5 1 5945 694 55 1267 1.5 1 1020 247 56 1213 1.5 1 1051 162 57 1763 1.5 1 1591 172 58 1226 1.5 1 1063 163 59 465 1.5 1 361 104 60 514 1.5 1 408 106 61 824 1.5 1 671 153 62 464 1.5 1 348 116 63 438 1.5 1 328 110 64 1678 1.5 1 1522 156 65 389 1.5 1 305 84 66 468 1.5 1 372 96 67 585 1.5 1 437 148 68 1729 1.5 1 1558 171 69 414 1.5 1 293 121 70 1074 1.5 1 922 152 71 1519 1.5 1 1304 215 72 2263 1.5 1 2044 219 73 1174 1.5 1 997 177 74 409 1.5 1 291 118 75 261 1.5 1 205 56 76 552 1.5 1 443 109 77 917 1.5 1 810 107 78 1226 1.5 1 1081 145 79 1291 1.5 1 1139 152 80 1241 1.5 1 1092 149 81 1270 1.5 1 1127 143 82 1221 1.5 1 1065 156 83 1068 1.5 1 923 145 84 1013 1.5 1 863 150 85 925 1.5 1 764 161 86 817 1.5 1 672 145 87 791 1.5 1 667 124 88 672 1.5 1 542 130 89 615 1.5 1 485 130 90 611 1.5 1 497 114 91 551 1.5 1 434 117 92 480 1.5 1 359 121 93 478 1.5 1 359 119 94 444 1.5 1 328 116 95 391 1.5 1 283 108 96 380 1.5 1 270 110 97 364 1.5 1 271 93 98 348 1.5 1 240 108 99 290 1.5 1 202 88 100 290 1.5 1 210 80 101 256 1.5 1 170 86 102 245 1.5 1 165 80 103 257 1.5 1 195 62 104 250 1.5 1 175 75 105 248 1.5 1 169 79 106 227 1.5 1 144 83 107 191 1.5 1 135 56 108 187 1.5 1 114 73 109 195 1.5 1 128 67 110 173 1.5 1 100 73 111 160 1.5 1 101 59 112 162 1.5 1 103 59 113 164 1.5 1 110 54 114 160 1.5 1 102 58 115 156 1.5 1 93 63 116 148 1.5 1 97 51 117 116 1.5 1 79 37 118 129 1.5 1 93 36 119 130 1.5 1 86 44 120 115 1.5 1 66 49 121 99 1.5 1 66 33 122 110 1.5 1 72 38 123 101 1.5 1 71 30 124 104 1.5 1 70 34 125 79 1.5 1 61 18 126 86 1.5 1 59 27 127 72 1.5 1 38 34 128 68 1.5 1 44 24 129 70 1.5 1 49 21 130 70 1.5 1 54 16 131 46 1.5 1 30 16 132 50 1.5 1 35 15 133 38 1.5 1 27 11 134 40 1.5 1 30 10 135 22 1.5 1 15 7 136 24 1.5 1 16 8 137 19 1.5 1 8 11 138 12 1.5 1 7 5 139 12 1.5 1 11 1 140 16 1.5 1 12 4 141 15 1.5 1 9 6 142 12 1.5 1 7 5 143 7 1.5 1 0 7 144 13 1.5 1 2 11 145 19 1.5 1 8 11 146 14 1.5 1 6 8 147 16 1.5 1 4 12 148 16 1.5 1 4 12 149 38 1.5 1 3 35 150 388 1.5 1 2 386 RUN STATISTICS FOR INPUT FILE: ERR13913467_1.fastq.gz ============================================= 98078887 sequences processed in total