SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913466_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 40669). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913466_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2251.458 s (22.168 µs/read; 2.71 M reads/minute). === Summary === Total reads processed: 101,561,248 Reads with adapters: 43,150,732 (42.5%) Reads written (passing filters): 101,561,248 (100.0%) Total basepairs processed: 15,234,187,200 bp Quality-trimmed: 23,894,808 bp (0.2%) Total written (filtered): 15,007,406,577 bp (98.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 43150732 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.2% G: 13.3% T: 48.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 30973397 25390312.0 0 30973397 2 4521499 6347578.0 0 4521499 3 1834722 1586894.5 0 1834722 4 814963 396723.6 0 814963 5 143199 99180.9 0 143199 6 105245 24795.2 0 105245 7 114502 6198.8 0 114502 8 119371 1549.7 0 119371 9 117708 387.4 0 116859 849 10 115596 96.9 1 104919 10677 11 124992 24.2 1 107656 17336 12 116448 6.1 1 103626 12822 13 106595 1.5 1 93371 13224 14 113435 1.5 1 96943 16492 15 112561 1.5 1 97496 15065 16 119143 1.5 1 100795 18348 17 127559 1.5 1 109373 18186 18 115212 1.5 1 102236 12976 19 96622 1.5 1 85089 11533 20 105204 1.5 1 91489 13715 21 97478 1.5 1 85247 12231 22 93402 1.5 1 82722 10680 23 95413 1.5 1 83561 11852 24 95706 1.5 1 81893 13813 25 91227 1.5 1 80550 10677 26 91507 1.5 1 81007 10500 27 97529 1.5 1 85708 11821 28 91746 1.5 1 82067 9679 29 98695 1.5 1 85622 13073 30 92297 1.5 1 82609 9688 31 75884 1.5 1 67942 7942 32 80456 1.5 1 72118 8338 33 79196 1.5 1 70147 9049 34 78053 1.5 1 69586 8467 35 72626 1.5 1 65454 7172 36 77072 1.5 1 68548 8524 37 70365 1.5 1 64392 5973 38 78618 1.5 1 69518 9100 39 71422 1.5 1 64964 6458 40 69757 1.5 1 62381 7376 41 80361 1.5 1 69304 11057 42 55305 1.5 1 50904 4401 43 58625 1.5 1 53272 5353 44 57400 1.5 1 52582 4818 45 66790 1.5 1 58771 8019 46 57142 1.5 1 50884 6258 47 89661 1.5 1 81871 7790 48 31562 1.5 1 27676 3886 49 50792 1.5 1 46597 4195 50 86782 1.5 1 79750 7032 51 42763 1.5 1 38885 3878 52 53447 1.5 1 48254 5193 53 66996 1.5 1 60664 6332 54 37993 1.5 1 35048 2945 55 22671 1.5 1 21074 1597 56 52276 1.5 1 48731 3545 57 58861 1.5 1 55494 3367 58 30642 1.5 1 28207 2435 59 49025 1.5 1 46447 2578 60 18461 1.5 1 17089 1372 61 35760 1.5 1 33298 2462 62 18319 1.5 1 17282 1037 63 9412 1.5 1 8575 837 64 31353 1.5 1 29560 1793 65 27473 1.5 1 26153 1320 66 8688 1.5 1 7962 726 67 14485 1.5 1 13417 1068 68 28245 1.5 1 26787 1458 69 14706 1.5 1 13664 1042 70 23439 1.5 1 21900 1539 71 21232 1.5 1 19756 1476 72 31586 1.5 1 29470 2116 73 2586 1.5 1 2152 434 74 1776 1.5 1 1604 172 75 5579 1.5 1 5281 298 76 8913 1.5 1 8438 475 77 9951 1.5 1 9427 524 78 10106 1.5 1 9491 615 79 9779 1.5 1 9226 553 80 8974 1.5 1 8415 559 81 8358 1.5 1 7838 520 82 7527 1.5 1 7025 502 83 6242 1.5 1 5815 427 84 5430 1.5 1 5042 388 85 4661 1.5 1 4335 326 86 4080 1.5 1 3768 312 87 3628 1.5 1 3363 265 88 3175 1.5 1 2937 238 89 2870 1.5 1 2664 206 90 2496 1.5 1 2288 208 91 2114 1.5 1 1961 153 92 1683 1.5 1 1523 160 93 1422 1.5 1 1284 138 94 1267 1.5 1 1129 138 95 1007 1.5 1 904 103 96 842 1.5 1 754 88 97 688 1.5 1 608 80 98 629 1.5 1 551 78 99 485 1.5 1 408 77 100 455 1.5 1 378 77 101 408 1.5 1 336 72 102 336 1.5 1 262 74 103 315 1.5 1 254 61 104 267 1.5 1 215 52 105 217 1.5 1 166 51 106 189 1.5 1 139 50 107 188 1.5 1 136 52 108 192 1.5 1 141 51 109 162 1.5 1 123 39 110 138 1.5 1 90 48 111 151 1.5 1 97 54 112 136 1.5 1 95 41 113 108 1.5 1 77 31 114 134 1.5 1 95 39 115 127 1.5 1 89 38 116 128 1.5 1 83 45 117 96 1.5 1 63 33 118 110 1.5 1 83 27 119 97 1.5 1 68 29 120 105 1.5 1 79 26 121 88 1.5 1 70 18 122 113 1.5 1 73 40 123 82 1.5 1 55 27 124 113 1.5 1 81 32 125 81 1.5 1 54 27 126 77 1.5 1 58 19 127 82 1.5 1 60 22 128 56 1.5 1 41 15 129 75 1.5 1 52 23 130 79 1.5 1 67 12 131 53 1.5 1 42 11 132 40 1.5 1 30 10 133 25 1.5 1 18 7 134 48 1.5 1 32 16 135 38 1.5 1 31 7 136 27 1.5 1 20 7 137 28 1.5 1 20 8 138 20 1.5 1 19 1 139 25 1.5 1 14 11 140 29 1.5 1 26 3 141 21 1.5 1 11 10 142 13 1.5 1 8 5 143 15 1.5 1 10 5 144 27 1.5 1 21 6 145 14 1.5 1 8 6 146 25 1.5 1 14 11 147 19 1.5 1 10 9 148 17 1.5 1 8 9 149 35 1.5 1 5 30 150 365 1.5 1 11 354 RUN STATISTICS FOR INPUT FILE: ERR13913466_1.fastq.gz ============================================= 101561248 sequences processed in total