SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913465_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 16165). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913465_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2007.381 s (20.281 µs/read; 2.96 M reads/minute). === Summary === Total reads processed: 98,977,739 Reads with adapters: 39,819,684 (40.2%) Reads written (passing filters): 98,977,739 (100.0%) Total basepairs processed: 14,846,660,850 bp Quality-trimmed: 21,523,503 bp (0.1%) Total written (filtered): 14,722,785,881 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 39819684 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.5% C: 0.1% G: 13.8% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 30753501 24744434.8 0 30753501 2 4695372 6186108.7 0 4695372 3 1807626 1546527.2 0 1807626 4 733785 386631.8 0 733785 5 58193 96657.9 0 58193 6 41756 24164.5 0 41756 7 44528 6041.1 0 44528 8 45758 1510.3 0 45758 9 46171 377.6 0 45288 883 10 47926 94.4 1 42145 5781 11 50744 23.6 1 43126 7618 12 48322 5.9 1 42588 5734 13 43800 1.5 1 38242 5558 14 46273 1.5 1 39521 6752 15 43642 1.5 1 37644 5998 16 45398 1.5 1 38176 7222 17 47423 1.5 1 40354 7069 18 42643 1.5 1 37698 4945 19 37049 1.5 1 32376 4673 20 41042 1.5 1 35601 5441 21 39864 1.5 1 34788 5076 22 38113 1.5 1 33657 4456 23 38930 1.5 1 33980 4950 24 38109 1.5 1 32365 5744 25 36360 1.5 1 31887 4473 26 34773 1.5 1 30553 4220 27 36039 1.5 1 31554 4485 28 33669 1.5 1 30088 3581 29 33926 1.5 1 29261 4665 30 33193 1.5 1 29555 3638 31 28090 1.5 1 25089 3001 32 29907 1.5 1 26763 3144 33 29988 1.5 1 26231 3757 34 31500 1.5 1 27615 3885 35 30463 1.5 1 27492 2971 36 21340 1.5 1 19264 2076 37 26013 1.5 1 23304 2709 38 24606 1.5 1 21820 2786 39 24997 1.5 1 22288 2709 40 22219 1.5 1 20026 2193 41 22707 1.5 1 20565 2142 42 21536 1.5 1 19539 1997 43 22993 1.5 1 20756 2237 44 21027 1.5 1 18849 2178 45 20578 1.5 1 17663 2915 46 19712 1.5 1 17241 2471 47 28001 1.5 1 25551 2450 48 10224 1.5 1 8828 1396 49 15035 1.5 1 13844 1191 50 22284 1.5 1 20327 1957 51 13959 1.5 1 12635 1324 52 15209 1.5 1 13627 1582 53 20058 1.5 1 17860 2198 54 12988 1.5 1 11786 1202 55 7965 1.5 1 7416 549 56 13623 1.5 1 12613 1010 57 16633 1.5 1 15578 1055 58 9615 1.5 1 8697 918 59 13528 1.5 1 12749 779 60 6924 1.5 1 6346 578 61 10361 1.5 1 9441 920 62 6148 1.5 1 5810 338 63 3820 1.5 1 3450 370 64 9911 1.5 1 9275 636 65 8424 1.5 1 8000 424 66 3630 1.5 1 3291 339 67 4720 1.5 1 4281 439 68 8380 1.5 1 7848 532 69 4709 1.5 1 4340 369 70 6948 1.5 1 6422 526 71 6137 1.5 1 5649 488 72 9351 1.5 1 8652 699 73 773 1.5 1 583 190 74 704 1.5 1 597 107 75 1945 1.5 1 1812 133 76 2927 1.5 1 2739 188 77 3288 1.5 1 3054 234 78 3121 1.5 1 2863 258 79 2984 1.5 1 2763 221 80 2927 1.5 1 2695 232 81 2656 1.5 1 2428 228 82 2430 1.5 1 2215 215 83 2312 1.5 1 2109 203 84 2101 1.5 1 1895 206 85 1928 1.5 1 1742 186 86 1772 1.5 1 1585 187 87 1548 1.5 1 1387 161 88 1373 1.5 1 1221 152 89 1283 1.5 1 1140 143 90 1153 1.5 1 1029 124 91 1031 1.5 1 895 136 92 970 1.5 1 841 129 93 821 1.5 1 689 132 94 714 1.5 1 608 106 95 656 1.5 1 551 105 96 579 1.5 1 485 94 97 515 1.5 1 407 108 98 479 1.5 1 392 87 99 415 1.5 1 325 90 100 367 1.5 1 278 89 101 314 1.5 1 231 83 102 318 1.5 1 245 73 103 289 1.5 1 226 63 104 256 1.5 1 176 80 105 235 1.5 1 172 63 106 227 1.5 1 168 59 107 187 1.5 1 124 63 108 214 1.5 1 152 62 109 186 1.5 1 121 65 110 187 1.5 1 131 56 111 152 1.5 1 98 54 112 158 1.5 1 99 59 113 146 1.5 1 95 51 114 145 1.5 1 92 53 115 146 1.5 1 96 50 116 142 1.5 1 91 51 117 111 1.5 1 71 40 118 115 1.5 1 81 34 119 89 1.5 1 56 33 120 115 1.5 1 80 35 121 108 1.5 1 73 35 122 96 1.5 1 60 36 123 96 1.5 1 55 41 124 91 1.5 1 61 30 125 85 1.5 1 53 32 126 85 1.5 1 57 28 127 80 1.5 1 57 23 128 79 1.5 1 51 28 129 57 1.5 1 41 16 130 56 1.5 1 40 16 131 42 1.5 1 31 11 132 45 1.5 1 30 15 133 36 1.5 1 21 15 134 29 1.5 1 25 4 135 29 1.5 1 17 12 136 24 1.5 1 17 7 137 20 1.5 1 13 7 138 21 1.5 1 17 4 139 14 1.5 1 6 8 140 14 1.5 1 6 8 141 5 1.5 1 2 3 142 13 1.5 1 7 6 143 7 1.5 1 4 3 144 8 1.5 1 1 7 145 14 1.5 1 7 7 146 11 1.5 1 3 8 147 15 1.5 1 5 10 148 9 1.5 1 1 8 149 31 1.5 1 6 25 150 771 1.5 1 5 766 RUN STATISTICS FOR INPUT FILE: ERR13913465_1.fastq.gz ============================================= 98977739 sequences processed in total