SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913464_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 20177). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913464_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2387.882 s (21.350 µs/read; 2.81 M reads/minute). === Summary === Total reads processed: 111,846,018 Reads with adapters: 45,339,887 (40.5%) Reads written (passing filters): 111,846,018 (100.0%) Total basepairs processed: 16,776,902,700 bp Quality-trimmed: 24,015,495 bp (0.1%) Total written (filtered): 16,620,697,708 bp (99.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 45339887 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.5% C: 0.2% G: 13.7% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 34637385 27961504.5 0 34637385 2 5179890 6990376.1 0 5179890 3 2015155 1747594.0 0 2015155 4 845674 436898.5 0 845674 5 82691 109224.6 0 82691 6 59737 27306.2 0 59737 7 63389 6826.5 0 63389 8 66357 1706.6 0 66357 9 66519 426.7 0 65591 928 10 67065 106.7 1 59783 7282 11 73875 26.7 1 62803 11072 12 68997 6.7 1 61092 7905 13 63112 1.7 1 55544 7568 14 66252 1.7 1 56884 9368 15 63282 1.7 1 54771 8511 16 65045 1.7 1 55009 10036 17 67333 1.7 1 57825 9508 18 61892 1.7 1 55078 6814 19 53033 1.7 1 46659 6374 20 59112 1.7 1 51511 7601 21 56834 1.7 1 49793 7041 22 54641 1.7 1 48373 6268 23 55707 1.7 1 48702 7005 24 55989 1.7 1 47901 8088 25 52819 1.7 1 46597 6222 26 49859 1.7 1 44223 5636 27 51492 1.7 1 45125 6367 28 46688 1.7 1 41898 4790 29 50358 1.7 1 43611 6747 30 48278 1.7 1 43251 5027 31 42262 1.7 1 38127 4135 32 43543 1.7 1 39084 4459 33 43206 1.7 1 38229 4977 34 43910 1.7 1 38944 4966 35 38298 1.7 1 34670 3628 36 38747 1.7 1 35006 3741 37 39224 1.7 1 34797 4427 38 37527 1.7 1 33702 3825 39 36445 1.7 1 32942 3503 40 38871 1.7 1 34627 4244 41 29686 1.7 1 27336 2350 42 35007 1.7 1 30550 4457 43 32865 1.7 1 29744 3121 44 31640 1.7 1 28658 2982 45 38561 1.7 1 33423 5138 46 24795 1.7 1 21894 2901 47 48276 1.7 1 44002 4274 48 12108 1.7 1 10392 1716 49 25688 1.7 1 23333 2355 50 73187 1.7 1 68319 4868 51 21839 1.7 1 19833 2006 52 29739 1.7 1 27167 2572 53 35602 1.7 1 32599 3003 54 18335 1.7 1 16869 1466 55 7050 1.7 1 6517 533 56 27369 1.7 1 25865 1504 57 23113 1.7 1 22006 1107 58 7854 1.7 1 7147 707 59 15847 1.7 1 15089 758 60 4043 1.7 1 3628 415 61 11457 1.7 1 10599 858 62 4659 1.7 1 4302 357 63 2325 1.7 1 2031 294 64 12427 1.7 1 11749 678 65 8280 1.7 1 7900 380 66 2115 1.7 1 1871 244 67 4908 1.7 1 4490 418 68 10499 1.7 1 9968 531 69 4297 1.7 1 3933 364 70 7813 1.7 1 7246 567 71 7942 1.7 1 7377 565 72 12704 1.7 1 11906 798 73 1541 1.7 1 1280 261 74 736 1.7 1 615 121 75 1891 1.7 1 1745 146 76 3355 1.7 1 3146 209 77 3983 1.7 1 3730 253 78 4135 1.7 1 3862 273 79 3891 1.7 1 3598 293 80 3680 1.7 1 3415 265 81 3312 1.7 1 3068 244 82 3070 1.7 1 2804 266 83 2702 1.7 1 2458 244 84 2581 1.7 1 2338 243 85 2122 1.7 1 1932 190 86 2023 1.7 1 1834 189 87 1763 1.7 1 1598 165 88 1469 1.7 1 1320 149 89 1346 1.7 1 1172 174 90 1172 1.7 1 1021 151 91 1107 1.7 1 957 150 92 950 1.7 1 810 140 93 768 1.7 1 667 101 94 735 1.7 1 609 126 95 661 1.7 1 547 114 96 580 1.7 1 473 107 97 479 1.7 1 387 92 98 433 1.7 1 332 101 99 426 1.7 1 335 91 100 358 1.7 1 257 101 101 351 1.7 1 255 96 102 330 1.7 1 219 111 103 281 1.7 1 198 83 104 288 1.7 1 194 94 105 236 1.7 1 168 68 106 207 1.7 1 143 64 107 225 1.7 1 143 82 108 202 1.7 1 125 77 109 210 1.7 1 132 78 110 195 1.7 1 125 70 111 186 1.7 1 121 65 112 189 1.7 1 118 71 113 168 1.7 1 122 46 114 164 1.7 1 105 59 115 136 1.7 1 81 55 116 165 1.7 1 103 62 117 136 1.7 1 90 46 118 119 1.7 1 73 46 119 138 1.7 1 83 55 120 120 1.7 1 80 40 121 130 1.7 1 85 45 122 126 1.7 1 79 47 123 111 1.7 1 79 32 124 111 1.7 1 83 28 125 75 1.7 1 51 24 126 102 1.7 1 72 30 127 78 1.7 1 57 21 128 82 1.7 1 52 30 129 83 1.7 1 55 28 130 74 1.7 1 52 22 131 61 1.7 1 41 20 132 48 1.7 1 37 11 133 44 1.7 1 27 17 134 55 1.7 1 42 13 135 28 1.7 1 17 11 136 23 1.7 1 20 3 137 18 1.7 1 14 4 138 27 1.7 1 19 8 139 5 1.7 1 2 3 140 11 1.7 1 5 6 141 11 1.7 1 5 6 142 11 1.7 1 5 6 143 3 1.7 1 2 1 144 18 1.7 1 11 7 145 10 1.7 1 1 9 146 13 1.7 1 4 9 147 16 1.7 1 6 10 148 19 1.7 1 6 13 149 42 1.7 1 4 38 150 614 1.7 1 3 611 RUN STATISTICS FOR INPUT FILE: ERR13913464_1.fastq.gz ============================================= 111846018 sequences processed in total