Bismark report for: ERR13913463_val_1.fq.gz and ERR13913463_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 96816339 Number of paired-end alignments with a unique best hit: 50003391 Mapping efficiency: 51.6% Sequence pairs with no alignments under any condition: 39133945 Sequence pairs did not map uniquely: 7679003 Sequence pairs which were discarded because genomic sequence could not be extracted: 39 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25000213 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 25003139 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2461977370 Total methylated C's in CpG context: 33251538 Total methylated C's in CHG context: 1076943 Total methylated C's in CHH context: 3745504 Total methylated C's in Unknown context: 78352 Total unmethylated C's in CpG context: 259018859 Total unmethylated C's in CHG context: 406398048 Total unmethylated C's in CHH context: 1758486478 Total unmethylated C's in Unknown context: 7088981 C methylated in CpG context: 11.4% C methylated in CHG context: 0.3% C methylated in CHH context: 0.2% C methylated in Unknown context (CN or CHN): 1.1% Bismark completed in 0d 5h 8m 55s