SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913463_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 15121). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913463_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2100.190 s (21.688 µs/read; 2.77 M reads/minute). === Summary === Total reads processed: 96,836,254 Reads with adapters: 38,939,434 (40.2%) Reads written (passing filters): 96,836,254 (100.0%) Total basepairs processed: 14,525,438,100 bp Quality-trimmed: 20,529,149 bp (0.1%) Total written (filtered): 14,406,837,093 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 38939434 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.1% G: 13.6% T: 48.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 30102176 24209063.5 0 30102176 2 4527297 6052265.9 0 4527297 3 1757370 1513066.5 0 1757370 4 728329 378266.6 0 728329 5 64405 94566.7 0 64405 6 45899 23641.7 0 45899 7 48617 5910.4 0 48617 8 50510 1477.6 0 50510 9 50700 369.4 0 49849 851 10 51318 92.4 1 45671 5647 11 55426 23.1 1 47246 8180 12 51044 5.8 1 45084 5960 13 45839 1.4 1 39691 6148 14 47994 1.4 1 40643 7351 15 46073 1.4 1 39448 6625 16 47211 1.4 1 39210 8001 17 49430 1.4 1 41911 7519 18 44751 1.4 1 39390 5361 19 38519 1.4 1 33409 5110 20 42550 1.4 1 36482 6068 21 40252 1.4 1 34738 5514 22 38541 1.4 1 33769 4772 23 39453 1.4 1 34136 5317 24 39558 1.4 1 33504 6054 25 37400 1.4 1 32453 4947 26 35176 1.4 1 30668 4508 27 36508 1.4 1 31800 4708 28 32848 1.4 1 29141 3707 29 35707 1.4 1 30612 5095 30 34139 1.4 1 30163 3976 31 28994 1.4 1 25583 3411 32 30218 1.4 1 26830 3388 33 30188 1.4 1 26162 4026 34 31089 1.4 1 27039 4050 35 25679 1.4 1 22957 2722 36 26450 1.4 1 23722 2728 37 24186 1.4 1 21618 2568 38 24065 1.4 1 21160 2905 39 23164 1.4 1 20840 2324 40 24778 1.4 1 22071 2707 41 20991 1.4 1 18267 2724 42 21205 1.4 1 18834 2371 43 19046 1.4 1 17260 1786 44 21749 1.4 1 19673 2076 45 16933 1.4 1 14710 2223 46 19143 1.4 1 16722 2421 47 23969 1.4 1 21901 2068 48 9804 1.4 1 8475 1329 49 13196 1.4 1 12125 1071 50 15349 1.4 1 13925 1424 51 14068 1.4 1 12775 1293 52 14102 1.4 1 12548 1554 53 15061 1.4 1 13191 1870 54 12465 1.4 1 11300 1165 55 9440 1.4 1 8663 777 56 10389 1.4 1 9474 915 57 11532 1.4 1 10559 973 58 9198 1.4 1 8265 933 59 10539 1.4 1 9658 881 60 9858 1.4 1 8976 882 61 8135 1.4 1 7252 883 62 7154 1.4 1 6646 508 63 5705 1.4 1 5179 526 64 7812 1.4 1 7109 703 65 7031 1.4 1 6547 484 66 5406 1.4 1 4934 472 67 4152 1.4 1 3737 415 68 6181 1.4 1 5726 455 69 5440 1.4 1 4945 495 70 6784 1.4 1 6231 553 71 4829 1.4 1 4329 500 72 6589 1.4 1 5996 593 73 542 1.4 1 425 117 74 636 1.4 1 552 84 75 1509 1.4 1 1355 154 76 2144 1.4 1 1983 161 77 2201 1.4 1 2025 176 78 2149 1.4 1 1946 203 79 1961 1.4 1 1759 202 80 1853 1.4 1 1676 177 81 1754 1.4 1 1555 199 82 1495 1.4 1 1325 170 83 1382 1.4 1 1240 142 84 1251 1.4 1 1106 145 85 1093 1.4 1 963 130 86 993 1.4 1 871 122 87 859 1.4 1 726 133 88 806 1.4 1 699 107 89 727 1.4 1 614 113 90 582 1.4 1 480 102 91 575 1.4 1 474 101 92 570 1.4 1 468 102 93 486 1.4 1 392 94 94 453 1.4 1 369 84 95 382 1.4 1 289 93 96 338 1.4 1 252 86 97 291 1.4 1 208 83 98 287 1.4 1 195 92 99 247 1.4 1 170 77 100 237 1.4 1 167 70 101 222 1.4 1 159 63 102 216 1.4 1 153 63 103 182 1.4 1 126 56 104 213 1.4 1 146 67 105 201 1.4 1 127 74 106 171 1.4 1 114 57 107 171 1.4 1 116 55 108 153 1.4 1 95 58 109 121 1.4 1 85 36 110 167 1.4 1 109 58 111 136 1.4 1 96 40 112 133 1.4 1 85 48 113 120 1.4 1 81 39 114 118 1.4 1 70 48 115 104 1.4 1 63 41 116 135 1.4 1 76 59 117 107 1.4 1 73 34 118 115 1.4 1 71 44 119 95 1.4 1 60 35 120 104 1.4 1 71 33 121 93 1.4 1 64 29 122 90 1.4 1 60 30 123 83 1.4 1 51 32 124 72 1.4 1 46 26 125 75 1.4 1 47 28 126 94 1.4 1 67 27 127 65 1.4 1 44 21 128 69 1.4 1 41 28 129 50 1.4 1 30 20 130 53 1.4 1 43 10 131 44 1.4 1 30 14 132 32 1.4 1 22 10 133 30 1.4 1 21 9 134 46 1.4 1 35 11 135 25 1.4 1 17 8 136 28 1.4 1 22 6 137 25 1.4 1 18 7 138 14 1.4 1 10 4 139 18 1.4 1 11 7 140 9 1.4 1 5 4 141 12 1.4 1 5 7 142 15 1.4 1 10 5 143 10 1.4 1 5 5 144 12 1.4 1 10 2 145 14 1.4 1 5 9 146 15 1.4 1 8 7 147 15 1.4 1 7 8 148 13 1.4 1 5 8 149 38 1.4 1 5 33 150 382 1.4 1 3 379 RUN STATISTICS FOR INPUT FILE: ERR13913463_1.fastq.gz ============================================= 96836254 sequences processed in total