SUMMARISING RUN PARAMETERS ========================== Input filename: ERR13913462_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 34166). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR13913462_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2540.877 s (21.435 µs/read; 2.80 M reads/minute). === Summary === Total reads processed: 118,537,385 Reads with adapters: 49,534,313 (41.8%) Reads written (passing filters): 118,537,385 (100.0%) Total basepairs processed: 17,780,607,750 bp Quality-trimmed: 27,071,677 bp (0.2%) Total written (filtered): 17,551,542,694 bp (98.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 49534313 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.2% C: 0.2% G: 14.0% T: 48.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 35921076 29634346.2 0 35921076 2 5435215 7408586.6 0 5435215 3 2172918 1852146.6 0 2172918 4 962309 463036.7 0 962309 5 163561 115759.2 0 163561 6 119953 28939.8 0 119953 7 129810 7234.9 0 129810 8 137696 1808.7 0 137696 9 136733 452.2 0 135712 1021 10 138121 113.0 1 125071 13050 11 148968 28.3 1 127538 21430 12 131426 7.1 1 116825 14601 13 113390 1.8 1 98659 14731 14 116275 1.8 1 99006 17269 15 111632 1.8 1 95685 15947 16 116900 1.8 1 97952 18948 17 128241 1.8 1 108738 19503 18 120492 1.8 1 106036 14456 19 101876 1.8 1 88745 13131 20 110261 1.8 1 95506 14755 21 102651 1.8 1 89345 13306 22 96342 1.8 1 84842 11500 23 96069 1.8 1 83406 12663 24 97433 1.8 1 82567 14866 25 93592 1.8 1 81640 11952 26 90582 1.8 1 79696 10886 27 95990 1.8 1 83611 12379 28 92238 1.8 1 82161 10077 29 104645 1.8 1 90299 14346 30 107703 1.8 1 95825 11878 31 94016 1.8 1 83675 10341 32 91564 1.8 1 81475 10089 33 83457 1.8 1 73177 10280 34 80657 1.8 1 71080 9577 35 70425 1.8 1 62839 7586 36 75754 1.8 1 66583 9171 37 68389 1.8 1 61681 6708 38 65673 1.8 1 59375 6298 39 83048 1.8 1 74155 8893 40 59464 1.8 1 52201 7263 41 69298 1.8 1 61984 7314 42 58361 1.8 1 53694 4667 43 62196 1.8 1 56018 6178 44 57068 1.8 1 50995 6073 45 60044 1.8 1 50399 9645 46 51167 1.8 1 45090 6077 47 86995 1.8 1 78596 8399 48 30346 1.8 1 26335 4011 49 47562 1.8 1 43004 4558 50 132262 1.8 1 122675 9587 51 43043 1.8 1 39028 4015 52 51977 1.8 1 47058 4919 53 64484 1.8 1 58860 5624 54 31588 1.8 1 29004 2584 55 12761 1.8 1 11712 1049 56 49739 1.8 1 46726 3013 57 42380 1.8 1 40140 2240 58 16654 1.8 1 15206 1448 59 31920 1.8 1 30224 1696 60 8473 1.8 1 7559 914 61 26335 1.8 1 24533 1802 62 9716 1.8 1 9033 683 63 4087 1.8 1 3624 463 64 22003 1.8 1 20758 1245 65 15521 1.8 1 14760 761 66 3530 1.8 1 3173 357 67 8698 1.8 1 7977 721 68 18619 1.8 1 17584 1035 69 7597 1.8 1 6948 649 70 14929 1.8 1 13839 1090 71 15622 1.8 1 14470 1152 72 23616 1.8 1 21999 1617 73 2503 1.8 1 2083 420 74 1075 1.8 1 920 155 75 3384 1.8 1 3152 232 76 5798 1.8 1 5455 343 77 6827 1.8 1 6367 460 78 6839 1.8 1 6374 465 79 6527 1.8 1 6087 440 80 6450 1.8 1 5999 451 81 6159 1.8 1 5710 449 82 5413 1.8 1 5041 372 83 4867 1.8 1 4484 383 84 3888 1.8 1 3587 301 85 3383 1.8 1 3110 273 86 2890 1.8 1 2679 211 87 2491 1.8 1 2278 213 88 2183 1.8 1 1949 234 89 2004 1.8 1 1800 204 90 1728 1.8 1 1551 177 91 1591 1.8 1 1432 159 92 1364 1.8 1 1200 164 93 1168 1.8 1 1021 147 94 976 1.8 1 850 126 95 820 1.8 1 715 105 96 659 1.8 1 561 98 97 609 1.8 1 496 113 98 544 1.8 1 433 111 99 454 1.8 1 381 73 100 412 1.8 1 314 98 101 364 1.8 1 287 77 102 350 1.8 1 267 83 103 294 1.8 1 233 61 104 286 1.8 1 209 77 105 206 1.8 1 150 56 106 213 1.8 1 159 54 107 213 1.8 1 147 66 108 201 1.8 1 140 61 109 189 1.8 1 140 49 110 181 1.8 1 129 52 111 164 1.8 1 110 54 112 156 1.8 1 107 49 113 151 1.8 1 105 46 114 148 1.8 1 108 40 115 150 1.8 1 103 47 116 131 1.8 1 95 36 117 128 1.8 1 88 40 118 144 1.8 1 94 50 119 112 1.8 1 79 33 120 121 1.8 1 85 36 121 103 1.8 1 74 29 122 125 1.8 1 90 35 123 137 1.8 1 104 33 124 104 1.8 1 76 28 125 124 1.8 1 94 30 126 110 1.8 1 78 32 127 84 1.8 1 65 19 128 99 1.8 1 72 27 129 83 1.8 1 68 15 130 85 1.8 1 64 21 131 90 1.8 1 62 28 132 63 1.8 1 49 14 133 56 1.8 1 46 10 134 73 1.8 1 47 26 135 41 1.8 1 29 12 136 37 1.8 1 26 11 137 40 1.8 1 27 13 138 34 1.8 1 26 8 139 33 1.8 1 27 6 140 28 1.8 1 20 8 141 22 1.8 1 11 11 142 26 1.8 1 16 10 143 18 1.8 1 8 10 144 20 1.8 1 11 9 145 22 1.8 1 15 7 146 25 1.8 1 15 10 147 21 1.8 1 12 9 148 25 1.8 1 15 10 149 41 1.8 1 11 30 150 500 1.8 1 10 490 RUN STATISTICS FOR INPUT FILE: ERR13913462_1.fastq.gz ============================================= 118537385 sequences processed in total