SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207073_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 37). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207073_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 771.062 s (14.590 µs/read; 4.11 M reads/minute). === Summary === Total reads processed: 52,847,841 Reads with adapters: 22,764,641 (43.1%) Reads written (passing filters): 52,847,841 (100.0%) Total basepairs processed: 6,663,716,444 bp Quality-trimmed: 2,077,880 bp (0.0%) Total written (filtered): 6,634,572,638 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 22764641 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.0% C: 10.4% G: 6.5% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 20139970 13211960.2 0 20139970 2 1741524 3302990.1 0 1741524 3 474041 825747.5 0 474041 4 299082 206436.9 0 299082 5 57704 51609.2 0 57704 6 15545 12902.3 0 15545 7 10772 3225.6 0 10772 8 8021 806.4 0 8021 9 2063 201.6 0 1607 456 10 13825 50.4 1 12748 1077 11 200 12.6 1 31 169 12 82 3.1 1 29 53 13 7 0.8 1 1 6 14 28 0.8 1 19 9 15 8 0.8 1 2 6 16 21 0.8 1 14 7 17 4 0.8 1 3 1 18 34 0.8 1 21 13 19 37 0.8 1 23 14 20 6 0.8 1 5 1 21 4 0.8 1 4 22 9 0.8 1 7 2 23 23 0.8 1 18 5 24 37 0.8 1 22 15 25 5 0.8 1 4 1 26 16 0.8 1 12 4 27 18 0.8 1 15 3 28 5 0.8 1 5 29 14 0.8 1 12 2 30 19 0.8 1 8 11 31 29 0.8 1 23 6 32 8 0.8 1 7 1 33 26 0.8 1 19 7 34 6 0.8 1 4 2 35 15 0.8 1 13 2 36 9 0.8 1 5 4 37 17 0.8 1 12 5 38 17 0.8 1 16 1 39 11 0.8 1 7 4 40 12 0.8 1 9 3 41 12 0.8 1 8 4 42 17 0.8 1 10 7 43 15 0.8 1 12 3 44 19 0.8 1 14 5 45 8 0.8 1 6 2 46 8 0.8 1 7 1 47 3 0.8 1 3 48 20 0.8 1 13 7 49 13 0.8 1 10 3 50 9 0.8 1 7 2 51 11 0.8 1 9 2 52 18 0.8 1 14 4 53 10 0.8 1 6 4 54 6 0.8 1 6 55 25 0.8 1 17 8 56 18 0.8 1 15 3 57 12 0.8 1 10 2 58 7 0.8 1 5 2 59 14 0.8 1 12 2 60 5 0.8 1 2 3 61 11 0.8 1 11 62 16 0.8 1 15 1 63 6 0.8 1 5 1 64 9 0.8 1 8 1 65 10 0.8 1 9 1 66 17 0.8 1 11 6 67 8 0.8 1 8 68 9 0.8 1 7 2 69 12 0.8 1 8 4 70 11 0.8 1 10 1 71 12 0.8 1 12 72 10 0.8 1 7 3 73 6 0.8 1 4 2 74 8 0.8 1 6 2 75 13 0.8 1 9 4 76 10 0.8 1 10 77 8 0.8 1 7 1 78 7 0.8 1 7 79 9 0.8 1 8 1 80 10 0.8 1 9 1 81 4 0.8 1 1 3 82 15 0.8 1 13 2 83 3 0.8 1 3 84 7 0.8 1 5 2 85 15 0.8 1 12 3 86 11 0.8 1 10 1 87 6 0.8 1 6 88 7 0.8 1 6 1 89 8 0.8 1 7 1 90 13 0.8 1 11 2 91 11 0.8 1 8 3 92 12 0.8 1 11 1 93 14 0.8 1 12 2 94 14 0.8 1 12 2 95 14 0.8 1 14 96 14 0.8 1 13 1 97 14 0.8 1 13 1 98 9 0.8 1 8 1 99 10 0.8 1 9 1 100 12 0.8 1 11 1 101 14 0.8 1 12 2 102 11 0.8 1 8 3 103 11 0.8 1 8 3 104 12 0.8 1 9 3 105 12 0.8 1 9 3 106 8 0.8 1 5 3 107 7 0.8 1 6 1 108 15 0.8 1 13 2 109 15 0.8 1 10 5 110 11 0.8 1 10 1 111 12 0.8 1 12 112 12 0.8 1 9 3 113 10 0.8 1 7 3 114 27 0.8 1 21 6 115 13 0.8 1 8 5 116 22 0.8 1 20 2 117 26 0.8 1 21 5 118 15 0.8 1 13 2 119 29 0.8 1 27 2 120 20 0.8 1 19 1 121 20 0.8 1 17 3 122 23 0.8 1 19 4 123 20 0.8 1 17 3 124 21 0.8 1 14 7 125 26 0.8 1 22 4 126 20 0.8 1 16 4 127 25 0.8 1 20 5 128 25 0.8 1 23 2 129 25 0.8 1 23 2 130 26 0.8 1 22 4 131 22 0.8 1 21 1 132 30 0.8 1 27 3 133 35 0.8 1 20 15 134 37 0.8 1 26 11 135 45 0.8 1 21 24 RUN STATISTICS FOR INPUT FILE: SRR13207073_1.fastq.gz ============================================= 52847841 sequences processed in total