SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207072_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 33). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207072_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 949.515 s (16.631 µs/read; 3.61 M reads/minute). === Summary === Total reads processed: 57,092,207 Reads with adapters: 24,597,705 (43.1%) Reads written (passing filters): 57,092,207 (100.0%) Total basepairs processed: 7,192,359,667 bp Quality-trimmed: 2,438,054 bp (0.0%) Total written (filtered): 7,160,901,486 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 24597705 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.4% C: 10.1% G: 6.2% T: 38.2% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 21938947 14273051.8 0 21938947 2 1725533 3568262.9 0 1725533 3 494996 892065.7 0 494996 4 325612 223016.4 0 325612 5 60444 55754.1 0 60444 6 15122 13938.5 0 15122 7 10970 3484.6 0 10970 8 8166 871.2 0 8166 9 2181 217.8 0 1742 439 10 13918 54.4 1 12775 1143 11 184 13.6 1 37 147 12 57 3.4 1 17 40 13 14 0.9 1 6 8 14 5 0.9 1 2 3 15 9 0.9 1 7 2 16 3 0.9 1 2 1 17 3 0.9 1 0 3 18 6 0.9 1 4 2 19 9 0.9 1 6 3 20 9 0.9 1 7 2 21 10 0.9 1 4 6 22 8 0.9 1 4 4 23 4 0.9 1 3 1 24 8 0.9 1 6 2 25 12 0.9 1 8 4 26 4 0.9 1 3 1 27 7 0.9 1 6 1 28 5 0.9 1 4 1 29 6 0.9 1 4 2 30 7 0.9 1 3 4 31 8 0.9 1 6 2 32 6 0.9 1 4 2 33 5 0.9 1 3 2 34 6 0.9 1 5 1 35 7 0.9 1 2 5 36 5 0.9 1 3 2 37 8 0.9 1 7 1 38 9 0.9 1 5 4 39 7 0.9 1 3 4 40 8 0.9 1 7 1 41 3 0.9 1 2 1 42 4 0.9 1 3 1 43 7 0.9 1 6 1 44 8 0.9 1 6 2 45 9 0.9 1 7 2 46 8 0.9 1 6 2 47 4 0.9 1 3 1 48 2 0.9 1 1 1 49 16 0.9 1 14 2 50 8 0.9 1 7 1 51 6 0.9 1 4 2 52 9 0.9 1 4 5 53 9 0.9 1 8 1 54 6 0.9 1 3 3 55 5 0.9 1 3 2 56 17 0.9 1 12 5 57 12 0.9 1 8 4 58 9 0.9 1 8 1 59 5 0.9 1 5 60 8 0.9 1 8 61 7 0.9 1 6 1 62 7 0.9 1 5 2 63 9 0.9 1 6 3 64 7 0.9 1 6 1 65 8 0.9 1 5 3 66 13 0.9 1 9 4 67 9 0.9 1 5 4 68 6 0.9 1 5 1 69 15 0.9 1 13 2 70 10 0.9 1 8 2 71 8 0.9 1 5 3 72 6 0.9 1 4 2 73 9 0.9 1 5 4 74 8 0.9 1 7 1 75 9 0.9 1 9 76 13 0.9 1 10 3 77 6 0.9 1 5 1 78 10 0.9 1 4 6 79 12 0.9 1 11 1 80 18 0.9 1 16 2 81 7 0.9 1 7 82 1 0.9 1 1 83 12 0.9 1 8 4 84 3 0.9 1 3 85 14 0.9 1 12 2 86 10 0.9 1 9 1 87 7 0.9 1 6 1 88 9 0.9 1 9 89 12 0.9 1 11 1 90 10 0.9 1 9 1 91 18 0.9 1 16 2 92 17 0.9 1 11 6 93 8 0.9 1 7 1 94 18 0.9 1 14 4 95 10 0.9 1 7 3 96 17 0.9 1 12 5 97 13 0.9 1 11 2 98 17 0.9 1 17 99 11 0.9 1 11 100 5 0.9 1 5 101 13 0.9 1 11 2 102 19 0.9 1 16 3 103 9 0.9 1 8 1 104 15 0.9 1 14 1 105 13 0.9 1 9 4 106 12 0.9 1 12 107 15 0.9 1 13 2 108 12 0.9 1 11 1 109 9 0.9 1 7 2 110 18 0.9 1 16 2 111 21 0.9 1 19 2 112 9 0.9 1 7 2 113 27 0.9 1 18 9 114 21 0.9 1 16 5 115 22 0.9 1 18 4 116 15 0.9 1 13 2 117 17 0.9 1 15 2 118 20 0.9 1 19 1 119 17 0.9 1 14 3 120 17 0.9 1 14 3 121 25 0.9 1 19 6 122 22 0.9 1 17 5 123 21 0.9 1 19 2 124 20 0.9 1 14 6 125 24 0.9 1 22 2 126 20 0.9 1 16 4 127 31 0.9 1 28 3 128 29 0.9 1 25 4 129 33 0.9 1 27 6 130 41 0.9 1 34 7 131 41 0.9 1 37 4 132 32 0.9 1 30 2 133 52 0.9 1 40 12 134 45 0.9 1 34 11 135 56 0.9 1 31 25 RUN STATISTICS FOR INPUT FILE: SRR13207072_2.fastq.gz ============================================= 57092207 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 57092207 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 5784 (0.01%)