SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207072_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 33). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207072_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 940.265 s (16.469 µs/read; 3.64 M reads/minute). === Summary === Total reads processed: 57,092,207 Reads with adapters: 24,564,038 (43.0%) Reads written (passing filters): 57,092,207 (100.0%) Total basepairs processed: 7,194,811,854 bp Quality-trimmed: 2,160,013 bp (0.0%) Total written (filtered): 7,163,488,534 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 24564038 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.1% C: 10.4% G: 6.4% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 21756752 14273051.8 0 21756752 2 1861665 3568262.9 0 1861665 3 507444 892065.7 0 507444 4 321170 223016.4 0 321170 5 62573 55754.1 0 62573 6 16175 13938.5 0 16175 7 11234 3484.6 0 11234 8 8549 871.2 0 8549 9 2068 217.8 0 1619 449 10 14386 54.4 1 13213 1173 11 164 13.6 1 27 137 12 70 3.4 1 15 55 13 10 0.9 1 3 7 14 18 0.9 1 14 4 15 8 0.9 1 5 3 16 16 0.9 1 9 7 17 9 0.9 1 7 2 18 24 0.9 1 11 13 19 24 0.9 1 18 6 20 6 0.9 1 3 3 21 3 0.9 1 3 22 13 0.9 1 5 8 23 9 0.9 1 9 24 20 0.9 1 14 6 25 4 0.9 1 1 3 26 12 0.9 1 9 3 27 9 0.9 1 8 1 28 5 0.9 1 4 1 29 10 0.9 1 7 3 30 15 0.9 1 8 7 31 10 0.9 1 7 3 32 4 0.9 1 2 2 33 14 0.9 1 11 3 34 6 0.9 1 5 1 35 11 0.9 1 10 1 36 6 0.9 1 4 2 37 12 0.9 1 8 4 38 15 0.9 1 11 4 39 7 0.9 1 4 3 40 8 0.9 1 7 1 41 9 0.9 1 4 5 42 20 0.9 1 11 9 43 14 0.9 1 6 8 44 18 0.9 1 13 5 45 3 0.9 1 1 2 46 8 0.9 1 6 2 47 5 0.9 1 4 1 48 16 0.9 1 12 4 49 9 0.9 1 8 1 50 14 0.9 1 10 4 51 6 0.9 1 6 52 13 0.9 1 8 5 53 5 0.9 1 3 2 54 3 0.9 1 2 1 55 15 0.9 1 11 4 56 6 0.9 1 4 2 57 7 0.9 1 7 58 11 0.9 1 9 2 59 7 0.9 1 6 1 60 6 0.9 1 6 61 14 0.9 1 11 3 62 18 0.9 1 11 7 63 7 0.9 1 5 2 64 9 0.9 1 6 3 65 4 0.9 1 3 1 66 10 0.9 1 9 1 67 12 0.9 1 9 3 68 19 0.9 1 16 3 69 11 0.9 1 10 1 70 9 0.9 1 7 2 71 8 0.9 1 7 1 72 14 0.9 1 7 7 73 9 0.9 1 9 74 11 0.9 1 7 4 75 8 0.9 1 7 1 76 14 0.9 1 9 5 77 14 0.9 1 12 2 78 14 0.9 1 12 2 79 11 0.9 1 8 3 80 9 0.9 1 8 1 81 14 0.9 1 14 82 8 0.9 1 7 1 83 20 0.9 1 18 2 84 13 0.9 1 13 85 17 0.9 1 13 4 86 4 0.9 1 4 87 10 0.9 1 8 2 88 18 0.9 1 12 6 89 12 0.9 1 10 2 90 14 0.9 1 12 2 91 13 0.9 1 12 1 92 12 0.9 1 8 4 93 9 0.9 1 7 2 94 12 0.9 1 8 4 95 15 0.9 1 12 3 96 8 0.9 1 6 2 97 12 0.9 1 9 3 98 15 0.9 1 11 4 99 10 0.9 1 8 2 100 13 0.9 1 10 3 101 11 0.9 1 9 2 102 14 0.9 1 13 1 103 10 0.9 1 10 104 18 0.9 1 17 1 105 16 0.9 1 16 106 8 0.9 1 6 2 107 12 0.9 1 11 1 108 23 0.9 1 20 3 109 23 0.9 1 19 4 110 11 0.9 1 10 1 111 19 0.9 1 17 2 112 9 0.9 1 7 2 113 18 0.9 1 13 5 114 22 0.9 1 21 1 115 15 0.9 1 11 4 116 21 0.9 1 18 3 117 22 0.9 1 18 4 118 31 0.9 1 29 2 119 21 0.9 1 17 4 120 32 0.9 1 27 5 121 30 0.9 1 21 9 122 24 0.9 1 23 1 123 29 0.9 1 24 5 124 21 0.9 1 18 3 125 21 0.9 1 18 3 126 27 0.9 1 25 2 127 26 0.9 1 20 6 128 28 0.9 1 22 6 129 20 0.9 1 18 2 130 47 0.9 1 41 6 131 45 0.9 1 35 10 132 31 0.9 1 28 3 133 31 0.9 1 24 7 134 36 0.9 1 22 14 135 39 0.9 1 17 22 RUN STATISTICS FOR INPUT FILE: SRR13207072_1.fastq.gz ============================================= 57092207 sequences processed in total