SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207071_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 17). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207071_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 849.278 s (16.262 µs/read; 3.69 M reads/minute). === Summary === Total reads processed: 52,225,074 Reads with adapters: 22,053,906 (42.2%) Reads written (passing filters): 52,225,074 (100.0%) Total basepairs processed: 6,553,667,817 bp Quality-trimmed: 1,729,636 bp (0.0%) Total written (filtered): 6,525,541,744 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 22053906 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 44.9% C: 10.2% G: 6.6% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 19424719 13056268.5 0 19424719 2 1730165 3264067.1 0 1730165 3 461077 816016.8 0 461077 4 305022 204004.2 0 305022 5 70710 51001.0 0 70710 6 17504 12750.3 0 17504 7 12989 3187.6 0 12989 8 10246 796.9 0 10246 9 2676 199.2 0 2190 486 10 17881 49.8 1 16641 1240 11 149 12.5 1 16 133 12 40 3.1 1 8 32 13 4 0.8 1 1 3 14 1 0.8 1 0 1 16 3 0.8 1 1 2 17 2 0.8 1 1 1 18 5 0.8 1 0 5 19 5 0.8 1 2 3 20 1 0.8 1 0 1 21 2 0.8 1 0 2 22 4 0.8 1 0 4 23 4 0.8 1 2 2 24 1 0.8 1 1 25 4 0.8 1 2 2 26 2 0.8 1 1 1 27 4 0.8 1 0 4 28 2 0.8 1 0 2 29 2 0.8 1 1 1 30 3 0.8 1 0 3 31 3 0.8 1 2 1 32 2 0.8 1 0 2 33 3 0.8 1 2 1 34 1 0.8 1 0 1 35 5 0.8 1 5 36 1 0.8 1 1 38 2 0.8 1 2 39 5 0.8 1 2 3 40 3 0.8 1 2 1 41 2 0.8 1 0 2 42 3 0.8 1 2 1 43 2 0.8 1 1 1 44 2 0.8 1 1 1 45 2 0.8 1 2 46 3 0.8 1 1 2 47 1 0.8 1 1 48 3 0.8 1 1 2 49 8 0.8 1 7 1 50 3 0.8 1 3 52 1 0.8 1 1 53 2 0.8 1 0 2 54 3 0.8 1 2 1 55 2 0.8 1 0 2 56 6 0.8 1 3 3 57 2 0.8 1 2 58 3 0.8 1 2 1 59 2 0.8 1 2 60 2 0.8 1 2 61 1 0.8 1 1 62 2 0.8 1 2 63 1 0.8 1 1 64 4 0.8 1 2 2 65 3 0.8 1 2 1 66 11 0.8 1 9 2 67 3 0.8 1 3 68 2 0.8 1 1 1 70 2 0.8 1 2 71 3 0.8 1 1 2 72 7 0.8 1 7 73 2 0.8 1 1 1 74 6 0.8 1 3 3 75 3 0.8 1 2 1 76 4 0.8 1 3 1 77 1 0.8 1 1 78 4 0.8 1 4 79 4 0.8 1 3 1 80 5 0.8 1 5 81 4 0.8 1 3 1 82 2 0.8 1 2 83 4 0.8 1 4 84 6 0.8 1 6 85 3 0.8 1 2 1 86 5 0.8 1 2 3 87 4 0.8 1 2 2 88 2 0.8 1 2 89 1 0.8 1 0 1 90 2 0.8 1 0 2 91 8 0.8 1 8 92 8 0.8 1 7 1 93 3 0.8 1 2 1 94 5 0.8 1 5 95 11 0.8 1 10 1 96 10 0.8 1 5 5 97 7 0.8 1 3 4 98 11 0.8 1 9 2 99 7 0.8 1 7 100 5 0.8 1 4 1 101 6 0.8 1 5 1 102 5 0.8 1 4 1 103 4 0.8 1 3 1 104 5 0.8 1 2 3 105 8 0.8 1 8 106 4 0.8 1 4 107 13 0.8 1 11 2 108 12 0.8 1 10 2 109 3 0.8 1 3 110 12 0.8 1 10 2 111 9 0.8 1 6 3 112 4 0.8 1 1 3 113 11 0.8 1 8 3 114 12 0.8 1 11 1 115 4 0.8 1 3 1 116 10 0.8 1 9 1 117 10 0.8 1 8 2 118 8 0.8 1 5 3 119 20 0.8 1 14 6 120 14 0.8 1 10 4 121 11 0.8 1 9 2 122 10 0.8 1 6 4 123 7 0.8 1 6 1 124 10 0.8 1 6 4 125 15 0.8 1 13 2 126 11 0.8 1 11 127 6 0.8 1 5 1 128 15 0.8 1 13 2 129 17 0.8 1 12 5 130 24 0.8 1 20 4 131 23 0.8 1 21 2 132 19 0.8 1 18 1 133 25 0.8 1 20 5 134 29 0.8 1 24 5 135 30 0.8 1 15 15 RUN STATISTICS FOR INPUT FILE: SRR13207071_1.fastq.gz ============================================= 52225074 sequences processed in total