SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207070_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 17). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207070_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 968.340 s (17.829 µs/read; 3.37 M reads/minute). === Summary === Total reads processed: 54,311,933 Reads with adapters: 24,163,085 (44.5%) Reads written (passing filters): 54,311,933 (100.0%) Total basepairs processed: 6,898,362,841 bp Quality-trimmed: 4,325,943 bp (0.1%) Total written (filtered): 6,865,728,443 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 24163085 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 44.2% C: 11.4% G: 6.4% T: 37.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 21536201 13577983.2 0 21536201 2 1743756 3394495.8 0 1743756 3 527423 848624.0 0 527423 4 276862 212156.0 0 276862 5 40127 53039.0 0 40127 6 11848 13259.7 0 11848 7 8239 3314.9 0 8239 8 5344 828.7 0 5344 9 1714 207.2 0 1298 416 10 10370 51.8 1 9078 1292 11 145 12.9 1 18 127 12 38 3.2 1 3 35 13 12 0.8 1 1 11 15 1 0.8 1 0 1 16 2 0.8 1 0 2 17 3 0.8 1 1 2 18 8 0.8 1 4 4 19 5 0.8 1 2 3 20 3 0.8 1 2 1 21 3 0.8 1 1 2 22 7 0.8 1 4 3 23 2 0.8 1 1 1 24 2 0.8 1 2 25 1 0.8 1 1 26 3 0.8 1 0 3 27 3 0.8 1 2 1 28 4 0.8 1 0 4 29 2 0.8 1 1 1 30 3 0.8 1 2 1 31 3 0.8 1 1 2 32 5 0.8 1 2 3 33 1 0.8 1 1 34 3 0.8 1 2 1 35 2 0.8 1 2 36 2 0.8 1 2 37 1 0.8 1 0 1 38 2 0.8 1 1 1 39 3 0.8 1 3 40 2 0.8 1 2 41 6 0.8 1 5 1 42 4 0.8 1 2 2 43 5 0.8 1 3 2 44 3 0.8 1 3 45 4 0.8 1 3 1 46 2 0.8 1 1 1 47 4 0.8 1 1 3 48 2 0.8 1 2 49 1 0.8 1 0 1 50 8 0.8 1 8 51 4 0.8 1 4 52 4 0.8 1 4 53 5 0.8 1 4 1 54 4 0.8 1 3 1 55 3 0.8 1 3 56 2 0.8 1 2 57 4 0.8 1 2 2 58 3 0.8 1 1 2 59 1 0.8 1 0 1 60 5 0.8 1 2 3 61 4 0.8 1 2 2 62 5 0.8 1 4 1 63 6 0.8 1 6 64 7 0.8 1 5 2 65 2 0.8 1 0 2 66 4 0.8 1 2 2 67 2 0.8 1 2 68 1 0.8 1 1 69 7 0.8 1 5 2 70 1 0.8 1 0 1 71 3 0.8 1 3 72 6 0.8 1 5 1 73 3 0.8 1 1 2 74 7 0.8 1 6 1 75 2 0.8 1 1 1 76 8 0.8 1 7 1 77 3 0.8 1 1 2 78 3 0.8 1 3 79 11 0.8 1 8 3 80 3 0.8 1 3 81 8 0.8 1 7 1 82 2 0.8 1 1 1 83 10 0.8 1 5 5 84 13 0.8 1 13 85 5 0.8 1 2 3 86 3 0.8 1 1 2 87 5 0.8 1 2 3 88 8 0.8 1 5 3 89 13 0.8 1 13 90 9 0.8 1 8 1 91 7 0.8 1 5 2 92 6 0.8 1 5 1 93 4 0.8 1 2 2 94 3 0.8 1 2 1 95 6 0.8 1 6 96 7 0.8 1 5 2 97 6 0.8 1 6 98 7 0.8 1 7 99 5 0.8 1 1 4 100 12 0.8 1 11 1 101 8 0.8 1 7 1 102 10 0.8 1 7 3 103 12 0.8 1 10 2 104 8 0.8 1 7 1 105 10 0.8 1 8 2 106 10 0.8 1 9 1 107 4 0.8 1 4 108 8 0.8 1 6 2 109 9 0.8 1 9 110 6 0.8 1 5 1 111 21 0.8 1 12 9 112 15 0.8 1 14 1 113 14 0.8 1 13 1 114 11 0.8 1 10 1 115 14 0.8 1 12 2 116 9 0.8 1 9 117 13 0.8 1 11 2 118 20 0.8 1 15 5 119 22 0.8 1 21 1 120 18 0.8 1 17 1 121 17 0.8 1 12 5 122 15 0.8 1 10 5 123 19 0.8 1 17 2 124 25 0.8 1 18 7 125 20 0.8 1 14 6 126 22 0.8 1 19 3 127 24 0.8 1 20 4 128 27 0.8 1 23 4 129 28 0.8 1 24 4 130 19 0.8 1 16 3 131 30 0.8 1 20 10 132 32 0.8 1 27 5 133 40 0.8 1 34 6 134 29 0.8 1 20 9 135 40 0.8 1 18 22 RUN STATISTICS FOR INPUT FILE: SRR13207070_1.fastq.gz ============================================= 54311933 sequences processed in total