SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207069_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 14). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207069_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 797.954 s (15.012 µs/read; 4.00 M reads/minute). === Summary === Total reads processed: 53,154,280 Reads with adapters: 23,096,436 (43.5%) Reads written (passing filters): 53,154,280 (100.0%) Total basepairs processed: 6,723,235,587 bp Quality-trimmed: 2,278,769 bp (0.0%) Total written (filtered): 6,693,671,786 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 23096436 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.1% C: 10.5% G: 6.4% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 20502045 13288570.0 0 20502045 2 1722172 3322142.5 0 1722172 3 475946 830535.6 0 475946 4 293396 207633.9 0 293396 5 54450 51908.5 0 54450 6 14073 12977.1 0 14073 7 10189 3244.3 0 10189 8 7696 811.1 0 7696 9 2014 202.8 0 1595 419 10 13015 50.7 1 11941 1074 11 181 12.7 1 26 155 12 66 3.2 1 19 47 13 6 0.8 1 1 5 14 24 0.8 1 17 7 15 4 0.8 1 3 1 16 14 0.8 1 8 6 17 2 0.8 1 1 1 18 21 0.8 1 8 13 19 26 0.8 1 11 15 20 2 0.8 1 2 21 1 0.8 1 1 22 8 0.8 1 5 3 23 19 0.8 1 10 9 24 21 0.8 1 15 6 25 4 0.8 1 2 2 26 13 0.8 1 9 4 27 9 0.8 1 9 29 7 0.8 1 3 4 30 13 0.8 1 7 6 31 12 0.8 1 9 3 32 4 0.8 1 2 2 33 18 0.8 1 9 9 34 2 0.8 1 1 1 35 6 0.8 1 5 1 36 9 0.8 1 7 2 37 17 0.8 1 10 7 38 6 0.8 1 5 1 39 7 0.8 1 6 1 40 11 0.8 1 8 3 41 11 0.8 1 6 5 42 9 0.8 1 7 2 43 9 0.8 1 6 3 44 11 0.8 1 7 4 45 3 0.8 1 1 2 46 10 0.8 1 5 5 47 4 0.8 1 2 2 48 17 0.8 1 12 5 49 12 0.8 1 4 8 50 6 0.8 1 3 3 51 5 0.8 1 3 2 52 11 0.8 1 11 53 5 0.8 1 5 54 6 0.8 1 6 55 12 0.8 1 7 5 56 10 0.8 1 8 2 57 5 0.8 1 5 58 2 0.8 1 2 59 9 0.8 1 8 1 60 4 0.8 1 3 1 61 3 0.8 1 2 1 62 12 0.8 1 8 4 63 1 0.8 1 1 64 3 0.8 1 1 2 66 13 0.8 1 11 2 67 8 0.8 1 7 1 68 9 0.8 1 8 1 69 5 0.8 1 2 3 70 5 0.8 1 5 71 10 0.8 1 6 4 72 7 0.8 1 5 2 73 5 0.8 1 4 1 74 2 0.8 1 2 75 8 0.8 1 7 1 76 2 0.8 1 1 1 77 9 0.8 1 7 2 78 9 0.8 1 6 3 79 1 0.8 1 0 1 80 5 0.8 1 4 1 81 6 0.8 1 4 2 82 7 0.8 1 5 2 83 2 0.8 1 1 1 84 4 0.8 1 4 85 7 0.8 1 7 86 6 0.8 1 5 1 87 10 0.8 1 9 1 88 5 0.8 1 4 1 89 11 0.8 1 9 2 90 8 0.8 1 7 1 91 6 0.8 1 5 1 92 8 0.8 1 6 2 93 4 0.8 1 4 94 3 0.8 1 3 95 4 0.8 1 4 96 6 0.8 1 6 97 4 0.8 1 4 98 7 0.8 1 7 99 4 0.8 1 4 100 6 0.8 1 4 2 101 7 0.8 1 7 102 4 0.8 1 1 3 103 6 0.8 1 6 104 5 0.8 1 4 1 105 7 0.8 1 7 106 8 0.8 1 7 1 107 15 0.8 1 10 5 108 17 0.8 1 12 5 109 15 0.8 1 10 5 110 6 0.8 1 5 1 111 5 0.8 1 4 1 112 11 0.8 1 11 113 13 0.8 1 10 3 114 11 0.8 1 9 2 115 8 0.8 1 5 3 116 5 0.8 1 5 117 8 0.8 1 6 2 118 10 0.8 1 7 3 119 12 0.8 1 10 2 120 15 0.8 1 14 1 121 26 0.8 1 22 4 122 26 0.8 1 21 5 123 9 0.8 1 6 3 124 19 0.8 1 16 3 125 19 0.8 1 13 6 126 15 0.8 1 15 127 13 0.8 1 12 1 128 29 0.8 1 26 3 129 32 0.8 1 29 3 130 12 0.8 1 10 2 131 22 0.8 1 20 2 132 31 0.8 1 26 5 133 22 0.8 1 18 4 134 17 0.8 1 12 5 135 31 0.8 1 15 16 RUN STATISTICS FOR INPUT FILE: SRR13207069_1.fastq.gz ============================================= 53154280 sequences processed in total