SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207068_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 32). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207068_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 810.559 s (15.789 µs/read; 3.80 M reads/minute). === Summary === Total reads processed: 51,337,098 Reads with adapters: 22,250,143 (43.3%) Reads written (passing filters): 51,337,098 (100.0%) Total basepairs processed: 6,502,868,062 bp Quality-trimmed: 1,736,389 bp (0.0%) Total written (filtered): 6,475,103,537 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 22250143 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.6% C: 10.2% G: 6.0% T: 38.1% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 19934151 12834274.5 0 19934151 2 1505115 3208568.6 0 1505115 3 442293 802142.2 0 442293 4 281742 200535.5 0 281742 5 47399 50133.9 0 47399 6 11600 12533.5 0 11600 7 8370 3133.4 0 8370 8 6041 783.3 0 6041 9 1708 195.8 0 1349 359 10 10424 49.0 1 9483 941 11 127 12.2 1 27 100 12 48 3.1 1 15 33 13 9 0.8 1 2 7 14 12 0.8 1 6 6 15 7 0.8 1 5 2 16 4 0.8 1 3 1 17 11 0.8 1 6 5 18 1 0.8 1 1 19 17 0.8 1 8 9 20 7 0.8 1 6 1 21 3 0.8 1 1 2 22 5 0.8 1 5 23 8 0.8 1 3 5 24 20 0.8 1 9 11 25 7 0.8 1 5 2 26 7 0.8 1 5 2 27 3 0.8 1 3 28 9 0.8 1 4 5 29 6 0.8 1 4 2 30 8 0.8 1 0 8 31 5 0.8 1 4 1 32 10 0.8 1 3 7 33 8 0.8 1 7 1 34 3 0.8 1 3 35 7 0.8 1 6 1 36 4 0.8 1 4 37 5 0.8 1 4 1 38 6 0.8 1 3 3 39 14 0.8 1 8 6 40 10 0.8 1 4 6 41 4 0.8 1 2 2 42 14 0.8 1 11 3 43 6 0.8 1 5 1 44 6 0.8 1 4 2 45 9 0.8 1 7 2 46 5 0.8 1 4 1 47 10 0.8 1 5 5 48 9 0.8 1 5 4 49 15 0.8 1 12 3 50 5 0.8 1 4 1 51 8 0.8 1 6 2 52 10 0.8 1 8 2 53 8 0.8 1 6 2 54 6 0.8 1 4 2 55 7 0.8 1 6 1 56 4 0.8 1 2 2 57 6 0.8 1 4 2 58 6 0.8 1 4 2 60 10 0.8 1 6 4 61 2 0.8 1 2 62 8 0.8 1 6 2 63 12 0.8 1 8 4 64 7 0.8 1 6 1 65 6 0.8 1 5 1 66 12 0.8 1 10 2 67 5 0.8 1 3 2 68 2 0.8 1 2 69 9 0.8 1 9 70 6 0.8 1 4 2 71 5 0.8 1 3 2 72 2 0.8 1 2 73 10 0.8 1 10 74 4 0.8 1 3 1 75 6 0.8 1 4 2 76 5 0.8 1 3 2 77 7 0.8 1 5 2 78 6 0.8 1 6 79 11 0.8 1 8 3 80 6 0.8 1 4 2 81 6 0.8 1 4 2 82 7 0.8 1 4 3 83 7 0.8 1 7 84 6 0.8 1 5 1 85 10 0.8 1 7 3 86 9 0.8 1 7 2 87 3 0.8 1 1 2 88 12 0.8 1 11 1 89 2 0.8 1 1 1 90 9 0.8 1 5 4 91 8 0.8 1 8 92 3 0.8 1 1 2 93 2 0.8 1 2 94 1 0.8 1 0 1 95 8 0.8 1 4 4 96 8 0.8 1 6 2 97 5 0.8 1 5 98 11 0.8 1 10 1 99 5 0.8 1 3 2 100 12 0.8 1 9 3 101 6 0.8 1 5 1 102 9 0.8 1 8 1 103 10 0.8 1 7 3 104 5 0.8 1 5 105 10 0.8 1 8 2 106 9 0.8 1 7 2 107 12 0.8 1 11 1 108 10 0.8 1 8 2 109 9 0.8 1 9 110 10 0.8 1 9 1 111 9 0.8 1 6 3 112 8 0.8 1 8 113 6 0.8 1 6 114 10 0.8 1 9 1 115 16 0.8 1 14 2 116 10 0.8 1 9 1 117 13 0.8 1 12 1 118 18 0.8 1 12 6 119 8 0.8 1 4 4 120 15 0.8 1 14 1 121 15 0.8 1 13 2 122 11 0.8 1 8 3 123 19 0.8 1 15 4 124 16 0.8 1 16 125 12 0.8 1 10 2 126 16 0.8 1 15 1 127 16 0.8 1 15 1 128 26 0.8 1 25 1 129 19 0.8 1 16 3 130 16 0.8 1 13 3 131 33 0.8 1 27 6 132 14 0.8 1 11 3 133 24 0.8 1 22 2 134 25 0.8 1 19 6 135 31 0.8 1 13 18 RUN STATISTICS FOR INPUT FILE: SRR13207068_2.fastq.gz ============================================= 51337098 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 51337098 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 4023 (0.01%)