SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207068_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 32). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207068_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 859.132 s (16.735 µs/read; 3.59 M reads/minute). === Summary === Total reads processed: 51,337,098 Reads with adapters: 22,468,074 (43.8%) Reads written (passing filters): 51,337,098 (100.0%) Total basepairs processed: 6,504,759,787 bp Quality-trimmed: 2,335,656 bp (0.0%) Total written (filtered): 6,475,942,769 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 22468074 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.0% C: 10.6% G: 6.3% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 19979505 12834274.5 0 19979505 2 1650411 3208568.6 0 1650411 3 470020 802142.2 0 470020 4 279846 200535.5 0 279846 5 46716 50133.9 0 46716 6 12187 12533.5 0 12187 7 8532 3133.4 0 8532 8 5998 783.3 0 5998 9 1655 195.8 0 1276 379 10 10922 49.0 1 9939 983 11 175 12.2 1 38 137 12 113 3.1 1 47 66 13 13 0.8 1 5 8 14 35 0.8 1 26 9 15 5 0.8 1 3 2 16 39 0.8 1 27 12 17 3 0.8 1 3 18 59 0.8 1 45 14 19 107 0.8 1 73 34 20 4 0.8 1 3 1 21 1 0.8 1 1 22 8 0.8 1 3 5 23 48 0.8 1 35 13 24 56 0.8 1 48 8 25 3 0.8 1 1 2 26 28 0.8 1 23 5 27 20 0.8 1 19 1 28 1 0.8 1 1 29 24 0.8 1 14 10 30 32 0.8 1 20 12 31 48 0.8 1 37 11 32 12 0.8 1 7 5 33 43 0.8 1 32 11 34 16 0.8 1 11 5 35 16 0.8 1 10 6 36 42 0.8 1 32 10 37 26 0.8 1 14 12 38 9 0.8 1 8 1 39 11 0.8 1 6 5 40 21 0.8 1 15 6 41 10 0.8 1 8 2 42 37 0.8 1 28 9 43 25 0.8 1 19 6 44 30 0.8 1 24 6 45 9 0.8 1 8 1 46 19 0.8 1 17 2 47 6 0.8 1 4 2 48 36 0.8 1 26 10 49 24 0.8 1 15 9 50 26 0.8 1 20 6 51 18 0.8 1 16 2 52 51 0.8 1 41 10 53 11 0.8 1 10 1 54 5 0.8 1 3 2 55 35 0.8 1 31 4 56 29 0.8 1 25 4 57 7 0.8 1 4 3 58 8 0.8 1 6 2 59 21 0.8 1 17 4 60 5 0.8 1 5 61 10 0.8 1 6 4 62 25 0.8 1 21 4 63 5 0.8 1 3 2 64 9 0.8 1 5 4 65 12 0.8 1 12 66 21 0.8 1 17 4 67 10 0.8 1 9 1 68 7 0.8 1 7 69 3 0.8 1 3 70 2 0.8 1 1 1 71 3 0.8 1 2 1 72 3 0.8 1 3 73 5 0.8 1 4 1 74 6 0.8 1 3 3 75 4 0.8 1 4 76 6 0.8 1 6 77 11 0.8 1 10 1 78 12 0.8 1 11 1 79 7 0.8 1 7 80 10 0.8 1 8 2 81 5 0.8 1 4 1 82 10 0.8 1 5 5 83 8 0.8 1 8 84 8 0.8 1 7 1 85 10 0.8 1 9 1 86 6 0.8 1 5 1 87 7 0.8 1 5 2 88 6 0.8 1 6 89 8 0.8 1 7 1 90 8 0.8 1 7 1 91 6 0.8 1 4 2 92 5 0.8 1 4 1 93 6 0.8 1 6 94 6 0.8 1 6 95 6 0.8 1 6 96 9 0.8 1 9 97 8 0.8 1 5 3 98 10 0.8 1 9 1 99 16 0.8 1 14 2 100 8 0.8 1 7 1 101 4 0.8 1 4 102 6 0.8 1 4 2 103 8 0.8 1 8 104 6 0.8 1 4 2 105 9 0.8 1 9 106 2 0.8 1 2 107 9 0.8 1 8 1 108 7 0.8 1 4 3 109 11 0.8 1 10 1 110 8 0.8 1 7 1 111 7 0.8 1 6 1 112 12 0.8 1 10 2 113 9 0.8 1 8 1 114 10 0.8 1 9 1 115 7 0.8 1 4 3 116 15 0.8 1 14 1 117 19 0.8 1 17 2 118 21 0.8 1 19 2 119 16 0.8 1 15 1 120 13 0.8 1 11 2 121 18 0.8 1 13 5 122 15 0.8 1 12 3 123 12 0.8 1 8 4 124 14 0.8 1 12 2 125 15 0.8 1 12 3 126 25 0.8 1 21 4 127 21 0.8 1 18 3 128 17 0.8 1 14 3 129 17 0.8 1 13 4 130 29 0.8 1 23 6 131 21 0.8 1 17 4 132 29 0.8 1 28 1 133 27 0.8 1 18 9 134 25 0.8 1 20 5 135 41 0.8 1 20 21 RUN STATISTICS FOR INPUT FILE: SRR13207068_1.fastq.gz ============================================= 51337098 sequences processed in total