SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207067_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 23). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207067_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 783.596 s (16.342 µs/read; 3.67 M reads/minute). === Summary === Total reads processed: 47,948,800 Reads with adapters: 20,718,810 (43.2%) Reads written (passing filters): 47,948,800 (100.0%) Total basepairs processed: 6,055,877,417 bp Quality-trimmed: 1,943,004 bp (0.0%) Total written (filtered): 6,029,422,407 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 20718810 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.1% C: 10.4% G: 6.4% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 18375993 11987200.0 0 18375993 2 1552544 2996800.0 0 1552544 3 429835 749200.0 0 429835 4 267870 187300.0 0 267870 5 49252 46825.0 0 49252 6 12776 11706.2 0 12776 7 9104 2926.6 0 9104 8 6650 731.6 0 6650 9 1753 182.9 0 1370 383 10 11436 45.7 1 10502 934 11 156 11.4 1 30 126 12 70 2.9 1 31 39 13 10 0.7 1 3 7 14 20 0.7 1 15 5 15 2 0.7 1 1 1 16 32 0.7 1 24 8 17 5 0.7 1 4 1 18 41 0.7 1 20 21 19 44 0.7 1 33 11 20 2 0.7 1 1 1 21 1 0.7 1 0 1 22 6 0.7 1 2 4 23 23 0.7 1 16 7 24 34 0.7 1 20 14 25 1 0.7 1 1 26 14 0.7 1 11 3 27 15 0.7 1 8 7 28 6 0.7 1 2 4 29 10 0.7 1 8 2 30 22 0.7 1 12 10 31 28 0.7 1 20 8 32 9 0.7 1 6 3 33 29 0.7 1 24 5 34 9 0.7 1 8 1 35 9 0.7 1 7 2 36 20 0.7 1 16 4 37 9 0.7 1 9 38 14 0.7 1 10 4 39 9 0.7 1 6 3 40 9 0.7 1 7 2 41 24 0.7 1 18 6 42 12 0.7 1 9 3 43 16 0.7 1 14 2 44 24 0.7 1 19 5 45 3 0.7 1 1 2 46 6 0.7 1 5 1 47 5 0.7 1 2 3 48 20 0.7 1 17 3 49 13 0.7 1 10 3 50 10 0.7 1 9 1 51 5 0.7 1 3 2 52 26 0.7 1 22 4 53 3 0.7 1 3 54 1 0.7 1 1 55 20 0.7 1 17 3 56 23 0.7 1 19 4 57 5 0.7 1 4 1 58 4 0.7 1 3 1 59 16 0.7 1 14 2 60 1 0.7 1 1 61 5 0.7 1 3 2 62 16 0.7 1 14 2 63 2 0.7 1 2 64 7 0.7 1 5 2 65 6 0.7 1 4 2 66 19 0.7 1 12 7 67 9 0.7 1 4 5 68 7 0.7 1 6 1 69 4 0.7 1 2 2 70 6 0.7 1 6 71 3 0.7 1 3 72 2 0.7 1 2 73 7 0.7 1 5 2 74 4 0.7 1 4 75 9 0.7 1 7 2 76 5 0.7 1 5 77 3 0.7 1 3 78 6 0.7 1 5 1 79 5 0.7 1 5 80 7 0.7 1 5 2 81 6 0.7 1 3 3 82 5 0.7 1 5 83 10 0.7 1 9 1 85 7 0.7 1 7 86 3 0.7 1 3 87 7 0.7 1 6 1 88 9 0.7 1 7 2 89 9 0.7 1 4 5 90 5 0.7 1 5 91 5 0.7 1 5 92 7 0.7 1 4 3 93 5 0.7 1 4 1 94 5 0.7 1 2 3 95 7 0.7 1 6 1 96 5 0.7 1 1 4 97 10 0.7 1 6 4 98 2 0.7 1 1 1 99 5 0.7 1 4 1 100 4 0.7 1 4 101 10 0.7 1 10 102 9 0.7 1 8 1 103 10 0.7 1 8 2 104 8 0.7 1 8 105 9 0.7 1 6 3 106 3 0.7 1 1 2 107 11 0.7 1 9 2 108 12 0.7 1 12 109 9 0.7 1 8 1 110 7 0.7 1 6 1 111 9 0.7 1 9 112 6 0.7 1 2 4 113 11 0.7 1 8 3 114 4 0.7 1 2 2 115 14 0.7 1 13 1 116 9 0.7 1 8 1 117 11 0.7 1 8 3 118 15 0.7 1 13 2 119 16 0.7 1 15 1 120 18 0.7 1 13 5 121 17 0.7 1 16 1 122 9 0.7 1 6 3 123 6 0.7 1 4 2 124 16 0.7 1 14 2 125 12 0.7 1 9 3 126 20 0.7 1 15 5 127 10 0.7 1 9 1 128 17 0.7 1 17 129 14 0.7 1 12 2 130 18 0.7 1 16 2 131 17 0.7 1 16 1 132 16 0.7 1 14 2 133 23 0.7 1 19 4 134 30 0.7 1 19 11 135 27 0.7 1 10 17 RUN STATISTICS FOR INPUT FILE: SRR13207067_1.fastq.gz ============================================= 47948800 sequences processed in total