SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207066_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 20). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207066_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 663.776 s (16.291 µs/read; 3.68 M reads/minute). === Summary === Total reads processed: 40,743,756 Reads with adapters: 17,444,950 (42.8%) Reads written (passing filters): 40,743,756 (100.0%) Total basepairs processed: 5,122,390,542 bp Quality-trimmed: 1,745,274 bp (0.0%) Total written (filtered): 5,099,945,601 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 17444950 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.0% C: 10.4% G: 6.4% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 15432601 10185939.0 0 15432601 2 1339323 2546484.8 0 1339323 3 361842 636621.2 0 361842 4 229699 159155.3 0 229699 5 43790 39788.8 0 43790 6 11029 9947.2 0 11029 7 8042 2486.8 0 8042 8 5919 621.7 0 5919 9 1553 155.4 0 1177 376 10 10046 38.9 1 9143 903 11 134 9.7 1 21 113 12 41 2.4 1 18 23 13 6 0.6 1 0 6 14 11 0.6 1 5 6 15 2 0.6 1 2 16 9 0.6 1 7 2 17 3 0.6 1 2 1 18 18 0.6 1 11 7 19 14 0.6 1 9 5 20 4 0.6 1 2 2 21 2 0.6 1 1 1 22 3 0.6 1 2 1 23 13 0.6 1 7 6 24 11 0.6 1 5 6 25 3 0.6 1 3 26 3 0.6 1 3 27 4 0.6 1 3 1 28 3 0.6 1 1 2 29 5 0.6 1 4 1 30 6 0.6 1 1 5 31 11 0.6 1 8 3 32 2 0.6 1 2 33 12 0.6 1 8 4 34 4 0.6 1 2 2 35 8 0.6 1 8 36 16 0.6 1 13 3 37 5 0.6 1 3 2 38 2 0.6 1 2 39 5 0.6 1 5 40 2 0.6 1 1 1 41 6 0.6 1 5 1 42 6 0.6 1 5 1 43 12 0.6 1 7 5 44 3 0.6 1 3 45 5 0.6 1 4 1 46 6 0.6 1 4 2 47 6 0.6 1 4 2 48 10 0.6 1 9 1 49 3 0.6 1 1 2 50 8 0.6 1 6 2 51 5 0.6 1 3 2 52 8 0.6 1 5 3 53 5 0.6 1 5 54 2 0.6 1 2 55 6 0.6 1 4 2 56 5 0.6 1 1 4 57 5 0.6 1 5 58 4 0.6 1 4 59 4 0.6 1 2 2 60 3 0.6 1 2 1 61 1 0.6 1 1 62 2 0.6 1 2 63 2 0.6 1 1 1 64 4 0.6 1 4 65 3 0.6 1 3 66 10 0.6 1 9 1 67 6 0.6 1 5 1 68 2 0.6 1 2 69 10 0.6 1 8 2 70 2 0.6 1 2 71 2 0.6 1 0 2 72 5 0.6 1 4 1 73 10 0.6 1 8 2 74 6 0.6 1 2 4 75 2 0.6 1 0 2 76 7 0.6 1 2 5 77 7 0.6 1 4 3 78 7 0.6 1 6 1 79 4 0.6 1 4 80 5 0.6 1 4 1 81 8 0.6 1 5 3 82 5 0.6 1 4 1 83 4 0.6 1 3 1 84 8 0.6 1 7 1 85 2 0.6 1 2 86 7 0.6 1 6 1 87 4 0.6 1 2 2 88 6 0.6 1 5 1 89 2 0.6 1 2 90 7 0.6 1 7 91 5 0.6 1 5 92 7 0.6 1 6 1 93 4 0.6 1 4 94 1 0.6 1 1 95 8 0.6 1 7 1 96 4 0.6 1 2 2 97 5 0.6 1 4 1 98 5 0.6 1 3 2 99 9 0.6 1 8 1 100 1 0.6 1 1 101 5 0.6 1 4 1 102 6 0.6 1 5 1 103 8 0.6 1 5 3 104 9 0.6 1 7 2 105 6 0.6 1 6 106 11 0.6 1 8 3 107 5 0.6 1 5 108 10 0.6 1 8 2 109 8 0.6 1 8 110 8 0.6 1 6 2 111 11 0.6 1 8 3 112 6 0.6 1 6 113 19 0.6 1 18 1 114 10 0.6 1 6 4 115 12 0.6 1 10 2 116 9 0.6 1 8 1 117 10 0.6 1 9 1 118 13 0.6 1 9 4 119 12 0.6 1 11 1 120 23 0.6 1 18 5 121 15 0.6 1 14 1 122 8 0.6 1 8 123 16 0.6 1 15 1 124 8 0.6 1 6 2 125 15 0.6 1 13 2 126 11 0.6 1 11 127 12 0.6 1 10 2 128 11 0.6 1 11 129 25 0.6 1 22 3 130 20 0.6 1 19 1 131 15 0.6 1 15 132 10 0.6 1 8 2 133 16 0.6 1 14 2 134 25 0.6 1 18 7 135 25 0.6 1 13 12 RUN STATISTICS FOR INPUT FILE: SRR13207066_1.fastq.gz ============================================= 40743756 sequences processed in total