SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207065_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207065_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 865.076 s (15.481 µs/read; 3.88 M reads/minute). === Summary === Total reads processed: 55,879,313 Reads with adapters: 24,629,147 (44.1%) Reads written (passing filters): 55,879,313 (100.0%) Total basepairs processed: 7,129,414,230 bp Quality-trimmed: 2,033,369 bp (0.0%) Total written (filtered): 7,098,489,178 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 24629147 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.3% C: 10.4% G: 6.2% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 21978299 13969828.2 0 21978299 2 1758396 3492457.1 0 1758396 3 499911 873114.3 0 499911 4 296834 218278.6 0 296834 5 49700 54569.6 0 49700 6 12916 13642.4 0 12916 7 9268 3410.6 0 9268 8 6943 852.7 0 6943 9 1916 213.2 0 1464 452 10 13264 53.3 1 12169 1095 11 183 13.3 1 31 152 12 82 3.3 1 20 62 13 15 0.8 1 3 12 14 28 0.8 1 19 9 15 3 0.8 1 2 1 16 22 0.8 1 14 8 17 5 0.8 1 1 4 18 25 0.8 1 17 8 19 32 0.8 1 23 9 20 2 0.8 1 0 2 21 9 0.8 1 4 5 22 4 0.8 1 3 1 23 25 0.8 1 15 10 24 26 0.8 1 14 12 25 5 0.8 1 3 2 26 9 0.8 1 6 3 27 11 0.8 1 9 2 28 2 0.8 1 1 1 29 9 0.8 1 6 3 30 20 0.8 1 12 8 31 23 0.8 1 14 9 32 6 0.8 1 4 2 33 23 0.8 1 22 1 34 9 0.8 1 4 5 35 8 0.8 1 6 2 36 14 0.8 1 12 2 37 12 0.8 1 7 5 38 22 0.8 1 16 6 39 14 0.8 1 12 2 40 11 0.8 1 10 1 41 15 0.8 1 10 5 42 8 0.8 1 5 3 43 17 0.8 1 15 2 44 21 0.8 1 17 4 45 2 0.8 1 0 2 46 6 0.8 1 2 4 47 3 0.8 1 2 1 48 22 0.8 1 18 4 49 15 0.8 1 10 5 50 4 0.8 1 2 2 51 8 0.8 1 6 2 52 16 0.8 1 14 2 53 2 0.8 1 1 1 54 2 0.8 1 2 55 17 0.8 1 12 5 56 14 0.8 1 12 2 57 4 0.8 1 2 2 58 9 0.8 1 8 1 59 12 0.8 1 8 4 60 10 0.8 1 9 1 61 8 0.8 1 8 62 12 0.8 1 9 3 63 6 0.8 1 4 2 64 8 0.8 1 4 4 65 8 0.8 1 8 66 7 0.8 1 6 1 67 7 0.8 1 7 68 9 0.8 1 6 3 69 6 0.8 1 3 3 70 3 0.8 1 2 1 71 7 0.8 1 7 72 3 0.8 1 0 3 73 5 0.8 1 4 1 74 9 0.8 1 7 2 75 5 0.8 1 5 76 5 0.8 1 2 3 77 9 0.8 1 7 2 78 7 0.8 1 5 2 79 8 0.8 1 7 1 80 6 0.8 1 4 2 81 13 0.8 1 11 2 82 6 0.8 1 6 83 6 0.8 1 4 2 84 6 0.8 1 4 2 85 5 0.8 1 5 86 8 0.8 1 4 4 87 7 0.8 1 6 1 88 11 0.8 1 10 1 89 4 0.8 1 4 90 8 0.8 1 6 2 91 8 0.8 1 6 2 92 9 0.8 1 7 2 93 11 0.8 1 7 4 94 6 0.8 1 4 2 95 6 0.8 1 3 3 96 9 0.8 1 8 1 97 10 0.8 1 7 3 98 3 0.8 1 3 99 9 0.8 1 9 100 6 0.8 1 4 2 101 6 0.8 1 5 1 102 9 0.8 1 6 3 103 6 0.8 1 4 2 104 10 0.8 1 8 2 105 20 0.8 1 16 4 106 7 0.8 1 5 2 107 13 0.8 1 13 108 13 0.8 1 8 5 109 8 0.8 1 7 1 110 7 0.8 1 6 1 111 13 0.8 1 11 2 112 14 0.8 1 12 2 113 10 0.8 1 9 1 114 9 0.8 1 6 3 115 13 0.8 1 11 2 116 18 0.8 1 15 3 117 13 0.8 1 11 2 118 16 0.8 1 13 3 119 12 0.8 1 12 120 18 0.8 1 17 1 121 14 0.8 1 12 2 122 8 0.8 1 6 2 123 14 0.8 1 12 2 124 17 0.8 1 15 2 125 12 0.8 1 10 2 126 19 0.8 1 18 1 127 14 0.8 1 13 1 128 23 0.8 1 23 129 24 0.8 1 22 2 130 16 0.8 1 16 131 21 0.8 1 20 1 132 31 0.8 1 24 7 133 29 0.8 1 20 9 134 29 0.8 1 23 6 135 39 0.8 1 17 22 RUN STATISTICS FOR INPUT FILE: SRR13207065_1.fastq.gz ============================================= 55879313 sequences processed in total