SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207064_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 32). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207064_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 663.333 s (16.742 µs/read; 3.58 M reads/minute). === Summary === Total reads processed: 39,620,531 Reads with adapters: 17,252,677 (43.5%) Reads written (passing filters): 39,620,531 (100.0%) Total basepairs processed: 5,016,985,237 bp Quality-trimmed: 1,682,604 bp (0.0%) Total written (filtered): 4,994,928,923 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 17252677 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.1% C: 10.5% G: 6.3% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 15338331 9905132.8 0 15338331 2 1270264 2476283.2 0 1270264 3 353363 619070.8 0 353363 4 216308 154767.7 0 216308 5 38896 38691.9 0 38896 6 10066 9673.0 0 10066 7 7187 2418.2 0 7187 8 5428 604.6 0 5428 9 1518 151.1 0 1212 306 10 9745 37.8 1 8989 756 11 140 9.4 1 34 106 12 77 2.4 1 29 48 13 13 0.6 1 2 11 14 12 0.6 1 7 5 15 5 0.6 1 3 2 16 15 0.6 1 9 6 17 1 0.6 1 1 18 28 0.6 1 20 8 19 31 0.6 1 22 9 20 4 0.6 1 1 3 21 5 0.6 1 2 3 22 7 0.6 1 4 3 23 22 0.6 1 13 9 24 24 0.6 1 16 8 26 15 0.6 1 10 5 27 12 0.6 1 10 2 28 1 0.6 1 1 29 8 0.6 1 3 5 30 19 0.6 1 13 6 31 21 0.6 1 16 5 32 2 0.6 1 2 33 14 0.6 1 9 5 34 5 0.6 1 3 2 35 12 0.6 1 10 2 36 11 0.6 1 8 3 37 11 0.6 1 4 7 38 8 0.6 1 6 2 39 12 0.6 1 10 2 40 11 0.6 1 9 2 41 13 0.6 1 8 5 42 8 0.6 1 8 43 15 0.6 1 9 6 44 10 0.6 1 9 1 45 5 0.6 1 4 1 46 10 0.6 1 7 3 47 5 0.6 1 3 2 48 12 0.6 1 9 3 49 13 0.6 1 10 3 50 6 0.6 1 4 2 51 8 0.6 1 6 2 52 17 0.6 1 13 4 53 11 0.6 1 10 1 54 6 0.6 1 4 2 55 15 0.6 1 10 5 56 15 0.6 1 13 2 57 4 0.6 1 3 1 58 10 0.6 1 8 2 59 10 0.6 1 7 3 60 4 0.6 1 4 61 1 0.6 1 1 62 9 0.6 1 7 2 63 8 0.6 1 7 1 64 4 0.6 1 3 1 65 7 0.6 1 4 3 66 12 0.6 1 8 4 67 8 0.6 1 7 1 68 4 0.6 1 3 1 69 2 0.6 1 1 1 70 3 0.6 1 2 1 71 11 0.6 1 11 72 2 0.6 1 2 73 10 0.6 1 9 1 74 6 0.6 1 3 3 75 9 0.6 1 6 3 76 6 0.6 1 5 1 77 5 0.6 1 5 78 9 0.6 1 7 2 79 11 0.6 1 11 80 7 0.6 1 6 1 81 8 0.6 1 8 82 7 0.6 1 4 3 83 5 0.6 1 3 2 84 11 0.6 1 10 1 85 10 0.6 1 7 3 86 9 0.6 1 8 1 87 8 0.6 1 6 2 88 6 0.6 1 4 2 89 7 0.6 1 7 90 5 0.6 1 4 1 91 12 0.6 1 11 1 92 7 0.6 1 7 93 11 0.6 1 11 94 11 0.6 1 8 3 95 9 0.6 1 8 1 96 8 0.6 1 4 4 97 7 0.6 1 6 1 98 8 0.6 1 7 1 99 7 0.6 1 7 100 8 0.6 1 8 101 9 0.6 1 8 1 102 10 0.6 1 8 2 103 13 0.6 1 9 4 104 9 0.6 1 8 1 105 9 0.6 1 5 4 106 12 0.6 1 12 107 6 0.6 1 6 108 14 0.6 1 12 2 109 8 0.6 1 7 1 110 11 0.6 1 10 1 111 8 0.6 1 7 1 112 14 0.6 1 14 113 10 0.6 1 10 114 6 0.6 1 5 1 115 12 0.6 1 9 3 116 6 0.6 1 5 1 117 15 0.6 1 13 2 118 10 0.6 1 9 1 119 14 0.6 1 12 2 120 17 0.6 1 17 121 14 0.6 1 13 1 122 14 0.6 1 8 6 123 15 0.6 1 13 2 124 13 0.6 1 13 125 9 0.6 1 5 4 126 26 0.6 1 23 3 127 14 0.6 1 11 3 128 30 0.6 1 30 129 29 0.6 1 28 1 130 26 0.6 1 23 3 131 21 0.6 1 19 2 132 26 0.6 1 19 7 133 20 0.6 1 19 1 134 21 0.6 1 11 10 135 39 0.6 1 16 23 RUN STATISTICS FOR INPUT FILE: SRR13207064_1.fastq.gz ============================================= 39620531 sequences processed in total