SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207063_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 30). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207063_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 921.530 s (18.347 µs/read; 3.27 M reads/minute). === Summary === Total reads processed: 50,228,886 Reads with adapters: 21,861,473 (43.5%) Reads written (passing filters): 50,228,886 (100.0%) Total basepairs processed: 6,355,763,675 bp Quality-trimmed: 2,416,042 bp (0.0%) Total written (filtered): 6,327,528,421 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 21861473 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.0% C: 10.5% G: 6.3% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 19428633 12557221.5 0 19428633 2 1609452 3139305.4 0 1609452 3 451732 784826.3 0 451732 4 273944 196206.6 0 273944 5 51805 49051.6 0 51805 6 13271 12262.9 0 13271 7 9186 3065.7 0 9186 8 6912 766.4 0 6912 9 2001 191.6 0 1610 391 10 12888 47.9 1 11793 1095 11 152 12.0 1 32 120 12 69 3.0 1 23 46 13 15 0.7 1 3 12 14 25 0.7 1 17 8 15 5 0.7 1 3 2 16 12 0.7 1 9 3 17 3 0.7 1 2 1 18 35 0.7 1 21 14 19 26 0.7 1 16 10 20 5 0.7 1 5 21 2 0.7 1 2 22 9 0.7 1 5 4 23 19 0.7 1 11 8 24 22 0.7 1 11 11 25 3 0.7 1 0 3 26 16 0.7 1 13 3 27 17 0.7 1 12 5 28 9 0.7 1 7 2 29 2 0.7 1 2 30 22 0.7 1 9 13 31 24 0.7 1 14 10 32 2 0.7 1 1 1 33 10 0.7 1 8 2 34 5 0.7 1 3 2 35 6 0.7 1 2 4 36 15 0.7 1 15 37 12 0.7 1 8 4 38 13 0.7 1 10 3 39 9 0.7 1 6 3 40 16 0.7 1 12 4 41 13 0.7 1 10 3 42 17 0.7 1 15 2 43 15 0.7 1 12 3 44 15 0.7 1 8 7 45 4 0.7 1 3 1 46 8 0.7 1 5 3 47 1 0.7 1 1 48 16 0.7 1 9 7 49 3 0.7 1 2 1 50 5 0.7 1 4 1 51 9 0.7 1 6 3 52 16 0.7 1 13 3 53 10 0.7 1 6 4 54 3 0.7 1 0 3 55 13 0.7 1 10 3 56 16 0.7 1 14 2 57 5 0.7 1 4 1 58 7 0.7 1 5 2 59 15 0.7 1 12 3 60 2 0.7 1 0 2 61 5 0.7 1 3 2 62 10 0.7 1 8 2 63 3 0.7 1 2 1 64 7 0.7 1 6 1 65 8 0.7 1 6 2 66 13 0.7 1 10 3 67 9 0.7 1 6 3 68 3 0.7 1 3 69 6 0.7 1 5 1 70 10 0.7 1 6 4 71 4 0.7 1 3 1 72 6 0.7 1 4 2 73 2 0.7 1 2 74 12 0.7 1 8 4 75 9 0.7 1 8 1 76 6 0.7 1 4 2 77 4 0.7 1 3 1 78 7 0.7 1 4 3 79 4 0.7 1 2 2 80 20 0.7 1 16 4 81 11 0.7 1 6 5 82 7 0.7 1 7 83 8 0.7 1 8 84 10 0.7 1 7 3 85 7 0.7 1 7 86 6 0.7 1 6 87 9 0.7 1 8 1 88 4 0.7 1 4 89 4 0.7 1 4 90 10 0.7 1 7 3 91 10 0.7 1 9 1 92 9 0.7 1 9 93 11 0.7 1 10 1 94 7 0.7 1 6 1 95 7 0.7 1 5 2 96 11 0.7 1 6 5 97 5 0.7 1 2 3 98 6 0.7 1 5 1 99 8 0.7 1 8 100 4 0.7 1 3 1 101 10 0.7 1 9 1 102 10 0.7 1 9 1 103 4 0.7 1 3 1 104 11 0.7 1 7 4 105 13 0.7 1 13 106 9 0.7 1 8 1 107 12 0.7 1 10 2 108 10 0.7 1 9 1 109 12 0.7 1 8 4 110 13 0.7 1 12 1 111 10 0.7 1 7 3 112 7 0.7 1 7 113 12 0.7 1 12 114 13 0.7 1 11 2 115 13 0.7 1 10 3 116 15 0.7 1 12 3 117 18 0.7 1 16 2 118 13 0.7 1 11 2 119 18 0.7 1 16 2 120 13 0.7 1 9 4 121 20 0.7 1 16 4 122 14 0.7 1 12 2 123 18 0.7 1 13 5 124 19 0.7 1 17 2 125 18 0.7 1 14 4 126 25 0.7 1 22 3 127 19 0.7 1 18 1 128 19 0.7 1 16 3 129 26 0.7 1 23 3 130 24 0.7 1 19 5 131 19 0.7 1 18 1 132 26 0.7 1 21 5 133 21 0.7 1 18 3 134 21 0.7 1 17 4 135 49 0.7 1 19 30 RUN STATISTICS FOR INPUT FILE: SRR13207063_1.fastq.gz ============================================= 50228886 sequences processed in total