SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207062_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 26). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207062_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 857.885 s (19.114 µs/read; 3.14 M reads/minute). === Summary === Total reads processed: 44,881,631 Reads with adapters: 19,722,224 (43.9%) Reads written (passing filters): 44,881,631 (100.0%) Total basepairs processed: 5,692,356,532 bp Quality-trimmed: 2,683,556 bp (0.0%) Total written (filtered): 5,666,410,876 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 19722224 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 44.7% C: 10.8% G: 6.4% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 17528792 11220407.8 0 17528792 2 1454610 2805101.9 0 1454610 3 418340 701275.5 0 418340 4 240375 175318.9 0 240375 5 41021 43829.7 0 41021 6 11374 10957.4 0 11374 7 7886 2739.4 0 7886 8 5720 684.8 0 5720 9 1679 171.2 0 1298 381 10 11099 42.8 1 10017 1082 11 132 10.7 1 24 108 12 49 2.7 1 16 33 13 7 0.7 1 2 5 14 10 0.7 1 3 7 15 2 0.7 1 1 1 16 10 0.7 1 8 2 17 1 0.7 1 0 1 18 12 0.7 1 6 6 19 15 0.7 1 9 6 20 3 0.7 1 1 2 21 3 0.7 1 1 2 22 4 0.7 1 2 2 23 8 0.7 1 7 1 24 4 0.7 1 4 25 2 0.7 1 1 1 26 6 0.7 1 4 2 27 10 0.7 1 7 3 28 5 0.7 1 2 3 29 5 0.7 1 4 1 30 6 0.7 1 2 4 31 6 0.7 1 4 2 32 5 0.7 1 4 1 33 8 0.7 1 4 4 34 2 0.7 1 0 2 35 6 0.7 1 4 2 36 11 0.7 1 5 6 37 3 0.7 1 2 1 38 8 0.7 1 5 3 39 6 0.7 1 6 40 3 0.7 1 3 41 5 0.7 1 2 3 42 4 0.7 1 3 1 43 8 0.7 1 7 1 44 9 0.7 1 7 2 45 3 0.7 1 2 1 46 7 0.7 1 3 4 47 2 0.7 1 1 1 48 6 0.7 1 4 2 49 11 0.7 1 7 4 50 5 0.7 1 5 51 4 0.7 1 3 1 52 8 0.7 1 5 3 53 6 0.7 1 4 2 54 4 0.7 1 3 1 55 11 0.7 1 8 3 56 7 0.7 1 3 4 57 4 0.7 1 4 58 3 0.7 1 2 1 59 6 0.7 1 5 1 61 3 0.7 1 1 2 62 5 0.7 1 5 63 3 0.7 1 3 64 2 0.7 1 2 65 7 0.7 1 5 2 66 6 0.7 1 4 2 67 6 0.7 1 6 68 5 0.7 1 5 69 4 0.7 1 4 70 2 0.7 1 2 71 2 0.7 1 1 1 72 3 0.7 1 3 73 9 0.7 1 8 1 74 8 0.7 1 4 4 75 6 0.7 1 6 76 11 0.7 1 10 1 77 3 0.7 1 2 1 78 11 0.7 1 7 4 79 6 0.7 1 5 1 81 2 0.7 1 2 82 9 0.7 1 7 2 83 7 0.7 1 7 84 6 0.7 1 3 3 85 8 0.7 1 8 86 9 0.7 1 6 3 87 12 0.7 1 11 1 88 4 0.7 1 3 1 89 9 0.7 1 8 1 90 13 0.7 1 13 91 4 0.7 1 4 92 7 0.7 1 5 2 93 3 0.7 1 3 94 8 0.7 1 7 1 95 9 0.7 1 9 96 10 0.7 1 9 1 97 9 0.7 1 8 1 98 13 0.7 1 11 2 99 14 0.7 1 11 3 100 14 0.7 1 13 1 101 8 0.7 1 5 3 102 11 0.7 1 10 1 103 7 0.7 1 6 1 104 10 0.7 1 9 1 105 8 0.7 1 7 1 106 7 0.7 1 6 1 107 12 0.7 1 11 1 108 10 0.7 1 10 109 10 0.7 1 9 1 110 18 0.7 1 16 2 111 14 0.7 1 13 1 112 14 0.7 1 11 3 113 13 0.7 1 11 2 114 13 0.7 1 9 4 115 13 0.7 1 10 3 116 4 0.7 1 3 1 117 10 0.7 1 8 2 118 12 0.7 1 10 2 119 14 0.7 1 11 3 120 12 0.7 1 12 121 21 0.7 1 17 4 122 17 0.7 1 16 1 123 15 0.7 1 9 6 124 22 0.7 1 21 1 125 12 0.7 1 12 126 33 0.7 1 28 5 127 27 0.7 1 22 5 128 30 0.7 1 26 4 129 26 0.7 1 22 4 130 28 0.7 1 25 3 131 26 0.7 1 20 6 132 26 0.7 1 24 2 133 27 0.7 1 19 8 134 28 0.7 1 18 10 135 33 0.7 1 15 18 RUN STATISTICS FOR INPUT FILE: SRR13207062_1.fastq.gz ============================================= 44881631 sequences processed in total