SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207061_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 25). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207061_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 724.283 s (16.786 µs/read; 3.57 M reads/minute). === Summary === Total reads processed: 43,149,301 Reads with adapters: 18,575,513 (43.0%) Reads written (passing filters): 43,149,301 (100.0%) Total basepairs processed: 5,444,670,140 bp Quality-trimmed: 1,595,123 bp (0.0%) Total written (filtered): 5,421,008,525 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 18575513 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.0% C: 10.5% G: 6.4% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 16448696 10787325.2 0 16448696 2 1401414 2696831.3 0 1401414 3 388555 674207.8 0 388555 4 244595 168552.0 0 244595 5 49532 42138.0 0 49532 6 12305 10534.5 0 12305 7 8768 2633.6 0 8768 8 6717 658.4 0 6717 9 1780 164.6 0 1436 344 10 11939 41.2 1 11083 856 11 130 10.3 1 21 109 12 38 2.6 1 12 26 13 8 0.6 1 3 5 14 4 0.6 1 3 1 15 2 0.6 1 1 1 16 7 0.6 1 3 4 17 3 0.6 1 2 1 18 9 0.6 1 6 3 19 15 0.6 1 9 6 20 4 0.6 1 4 21 2 0.6 1 2 22 4 0.6 1 3 1 23 8 0.6 1 5 3 24 2 0.6 1 2 25 3 0.6 1 3 26 4 0.6 1 2 2 27 7 0.6 1 6 1 28 3 0.6 1 2 1 29 6 0.6 1 4 2 30 4 0.6 1 3 1 31 11 0.6 1 6 5 32 3 0.6 1 3 33 5 0.6 1 3 2 34 3 0.6 1 2 1 35 1 0.6 1 0 1 36 2 0.6 1 1 1 37 6 0.6 1 3 3 38 5 0.6 1 4 1 39 2 0.6 1 2 40 3 0.6 1 2 1 41 2 0.6 1 1 1 42 1 0.6 1 1 43 11 0.6 1 7 4 44 4 0.6 1 3 1 45 3 0.6 1 2 1 46 5 0.6 1 3 2 47 4 0.6 1 2 2 48 5 0.6 1 5 49 7 0.6 1 3 4 50 5 0.6 1 3 2 51 4 0.6 1 4 52 3 0.6 1 3 53 7 0.6 1 4 3 54 3 0.6 1 2 1 55 5 0.6 1 5 56 5 0.6 1 3 2 57 9 0.6 1 8 1 58 1 0.6 1 1 59 6 0.6 1 5 1 60 9 0.6 1 6 3 61 4 0.6 1 4 62 7 0.6 1 6 1 63 3 0.6 1 3 64 4 0.6 1 4 65 4 0.6 1 3 1 66 7 0.6 1 6 1 67 2 0.6 1 1 1 68 5 0.6 1 3 2 69 8 0.6 1 5 3 70 6 0.6 1 6 71 3 0.6 1 3 72 13 0.6 1 8 5 73 6 0.6 1 5 1 74 6 0.6 1 4 2 75 9 0.6 1 2 7 76 8 0.6 1 4 4 77 4 0.6 1 4 78 6 0.6 1 6 79 1 0.6 1 1 80 9 0.6 1 5 4 81 6 0.6 1 4 2 82 8 0.6 1 7 1 83 6 0.6 1 4 2 84 7 0.6 1 6 1 85 12 0.6 1 9 3 86 2 0.6 1 1 1 87 4 0.6 1 2 2 88 11 0.6 1 9 2 89 11 0.6 1 10 1 90 12 0.6 1 10 2 91 7 0.6 1 6 1 92 8 0.6 1 7 1 93 7 0.6 1 7 94 8 0.6 1 6 2 95 5 0.6 1 5 96 9 0.6 1 8 1 97 8 0.6 1 5 3 98 3 0.6 1 3 99 6 0.6 1 4 2 100 5 0.6 1 5 101 5 0.6 1 4 1 102 9 0.6 1 5 4 103 5 0.6 1 5 104 9 0.6 1 6 3 105 8 0.6 1 6 2 106 8 0.6 1 6 2 107 10 0.6 1 5 5 108 10 0.6 1 8 2 109 9 0.6 1 7 2 110 7 0.6 1 7 111 11 0.6 1 7 4 112 21 0.6 1 18 3 113 11 0.6 1 9 2 114 11 0.6 1 10 1 115 9 0.6 1 8 1 116 11 0.6 1 8 3 117 20 0.6 1 19 1 118 8 0.6 1 6 2 119 8 0.6 1 7 1 120 11 0.6 1 10 1 121 15 0.6 1 14 1 122 21 0.6 1 16 5 123 17 0.6 1 13 4 124 15 0.6 1 15 125 19 0.6 1 16 3 126 21 0.6 1 19 2 127 23 0.6 1 20 3 128 21 0.6 1 18 3 129 25 0.6 1 21 4 130 23 0.6 1 20 3 131 23 0.6 1 22 1 132 24 0.6 1 21 3 133 37 0.6 1 28 9 134 25 0.6 1 16 9 135 39 0.6 1 14 25 RUN STATISTICS FOR INPUT FILE: SRR13207061_1.fastq.gz ============================================= 43149301 sequences processed in total