SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207060_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 24). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207060_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 798.855 s (16.688 µs/read; 3.60 M reads/minute). === Summary === Total reads processed: 47,871,006 Reads with adapters: 20,609,022 (43.1%) Reads written (passing filters): 47,871,006 (100.0%) Total basepairs processed: 6,044,965,124 bp Quality-trimmed: 1,661,047 bp (0.0%) Total written (filtered): 6,019,036,497 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 20609022 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.5% C: 10.1% G: 5.9% T: 38.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 18389974 11967751.5 0 18389974 2 1462519 2991937.9 0 1462519 3 393984 747984.5 0 393984 4 261257 186996.1 0 261257 5 55110 46749.0 0 55110 6 13822 11687.3 0 13822 7 9340 2921.8 0 9340 8 6830 730.5 0 6830 9 1917 182.6 0 1555 362 10 12674 45.7 1 11706 968 11 132 11.4 1 27 105 12 57 2.9 1 22 35 13 11 0.7 1 4 7 14 26 0.7 1 12 14 15 6 0.7 1 3 3 16 14 0.7 1 10 4 17 6 0.7 1 2 4 18 43 0.7 1 27 16 19 53 0.7 1 38 15 20 5 0.7 1 3 2 21 4 0.7 1 3 1 22 5 0.7 1 1 4 23 19 0.7 1 10 9 24 28 0.7 1 21 7 25 3 0.7 1 3 26 25 0.7 1 18 7 27 11 0.7 1 4 7 28 4 0.7 1 3 1 29 14 0.7 1 8 6 30 26 0.7 1 18 8 31 32 0.7 1 21 11 32 6 0.7 1 2 4 33 25 0.7 1 16 9 34 16 0.7 1 11 5 35 19 0.7 1 18 1 36 24 0.7 1 18 6 37 4 0.7 1 4 38 13 0.7 1 9 4 39 10 0.7 1 8 2 40 12 0.7 1 5 7 41 16 0.7 1 13 3 42 13 0.7 1 9 4 43 12 0.7 1 9 3 44 14 0.7 1 11 3 45 4 0.7 1 2 2 46 11 0.7 1 6 5 47 2 0.7 1 1 1 48 22 0.7 1 14 8 49 14 0.7 1 12 2 50 2 0.7 1 0 2 51 11 0.7 1 9 2 52 15 0.7 1 11 4 53 5 0.7 1 3 2 54 7 0.7 1 6 1 55 23 0.7 1 15 8 56 16 0.7 1 11 5 57 5 0.7 1 2 3 58 9 0.7 1 7 2 59 14 0.7 1 11 3 60 5 0.7 1 4 1 61 3 0.7 1 2 1 62 11 0.7 1 9 2 63 4 0.7 1 2 2 64 4 0.7 1 3 1 65 5 0.7 1 4 1 66 8 0.7 1 6 2 67 7 0.7 1 7 68 8 0.7 1 7 1 69 4 0.7 1 2 2 70 4 0.7 1 4 71 5 0.7 1 5 72 8 0.7 1 8 73 2 0.7 1 2 74 3 0.7 1 2 1 75 6 0.7 1 3 3 76 7 0.7 1 6 1 77 6 0.7 1 3 3 78 2 0.7 1 1 1 79 2 0.7 1 1 1 80 7 0.7 1 6 1 81 10 0.7 1 8 2 82 6 0.7 1 5 1 83 7 0.7 1 6 1 84 4 0.7 1 4 85 10 0.7 1 8 2 86 11 0.7 1 10 1 87 6 0.7 1 5 1 88 6 0.7 1 5 1 89 4 0.7 1 3 1 90 5 0.7 1 3 2 91 8 0.7 1 7 1 92 6 0.7 1 5 1 93 9 0.7 1 9 94 4 0.7 1 3 1 95 6 0.7 1 4 2 96 8 0.7 1 8 97 5 0.7 1 5 98 3 0.7 1 3 99 9 0.7 1 7 2 100 9 0.7 1 8 1 101 10 0.7 1 8 2 102 13 0.7 1 9 4 103 10 0.7 1 9 1 104 10 0.7 1 10 105 9 0.7 1 9 106 7 0.7 1 6 1 107 7 0.7 1 5 2 108 3 0.7 1 1 2 109 12 0.7 1 8 4 110 8 0.7 1 8 111 8 0.7 1 8 112 10 0.7 1 7 3 113 9 0.7 1 9 114 8 0.7 1 7 1 115 10 0.7 1 7 3 116 11 0.7 1 8 3 117 10 0.7 1 8 2 118 16 0.7 1 10 6 119 21 0.7 1 18 3 120 15 0.7 1 15 121 16 0.7 1 13 3 122 13 0.7 1 12 1 123 18 0.7 1 18 124 12 0.7 1 11 1 125 9 0.7 1 7 2 126 19 0.7 1 18 1 127 15 0.7 1 14 1 128 20 0.7 1 19 1 129 25 0.7 1 23 2 130 20 0.7 1 19 1 131 18 0.7 1 17 1 132 21 0.7 1 19 2 133 23 0.7 1 17 6 134 18 0.7 1 15 3 135 26 0.7 1 10 16 RUN STATISTICS FOR INPUT FILE: SRR13207060_1.fastq.gz ============================================= 47871006 sequences processed in total