SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207059_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 25). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207059_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 982.063 s (18.093 µs/read; 3.32 M reads/minute). === Summary === Total reads processed: 54,279,403 Reads with adapters: 23,503,336 (43.3%) Reads written (passing filters): 54,279,403 (100.0%) Total basepairs processed: 6,866,266,970 bp Quality-trimmed: 2,331,881 bp (0.0%) Total written (filtered): 6,836,137,936 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 23503336 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.1% C: 10.4% G: 6.4% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 20859514 13569850.8 0 20859514 2 1750993 3392462.7 0 1750993 3 487441 848115.7 0 487441 4 299944 212028.9 0 299944 5 55176 53007.2 0 55176 6 14518 13251.8 0 14518 7 10342 3313.0 0 10342 8 7594 828.2 0 7594 9 2061 207.1 0 1612 449 10 14212 51.8 1 13087 1125 11 164 12.9 1 37 127 12 66 3.2 1 24 42 13 6 0.8 1 1 5 14 13 0.8 1 7 6 15 4 0.8 1 3 1 16 11 0.8 1 7 4 18 32 0.8 1 17 15 19 26 0.8 1 18 8 20 8 0.8 1 5 3 21 6 0.8 1 5 1 22 1 0.8 1 0 1 23 11 0.8 1 7 4 24 18 0.8 1 13 5 25 1 0.8 1 0 1 26 8 0.8 1 5 3 27 7 0.8 1 5 2 28 4 0.8 1 3 1 29 5 0.8 1 2 3 30 14 0.8 1 9 5 31 15 0.8 1 11 4 32 3 0.8 1 3 33 17 0.8 1 12 5 34 4 0.8 1 1 3 35 6 0.8 1 3 3 36 5 0.8 1 5 37 8 0.8 1 2 6 38 6 0.8 1 3 3 39 5 0.8 1 3 2 40 10 0.8 1 9 1 41 7 0.8 1 2 5 42 11 0.8 1 5 6 43 11 0.8 1 8 3 44 7 0.8 1 5 2 45 4 0.8 1 2 2 46 4 0.8 1 4 47 4 0.8 1 3 1 48 10 0.8 1 5 5 49 7 0.8 1 5 2 50 4 0.8 1 3 1 51 4 0.8 1 3 1 52 11 0.8 1 6 5 53 8 0.8 1 6 2 54 6 0.8 1 6 55 14 0.8 1 8 6 56 15 0.8 1 11 4 57 8 0.8 1 4 4 58 2 0.8 1 2 59 4 0.8 1 4 60 3 0.8 1 1 2 61 3 0.8 1 3 62 12 0.8 1 9 3 63 3 0.8 1 3 64 3 0.8 1 3 65 10 0.8 1 8 2 66 9 0.8 1 7 2 67 2 0.8 1 2 68 8 0.8 1 6 2 69 5 0.8 1 2 3 70 5 0.8 1 4 1 71 3 0.8 1 3 72 9 0.8 1 8 1 73 3 0.8 1 3 74 5 0.8 1 2 3 75 6 0.8 1 6 76 11 0.8 1 8 3 77 4 0.8 1 3 1 78 9 0.8 1 7 2 79 5 0.8 1 4 1 80 5 0.8 1 4 1 81 9 0.8 1 9 82 5 0.8 1 4 1 83 8 0.8 1 4 4 84 7 0.8 1 6 1 85 8 0.8 1 7 1 86 9 0.8 1 9 87 8 0.8 1 7 1 88 9 0.8 1 8 1 89 4 0.8 1 4 90 9 0.8 1 8 1 91 12 0.8 1 8 4 92 6 0.8 1 6 93 6 0.8 1 4 2 94 8 0.8 1 5 3 95 7 0.8 1 5 2 96 10 0.8 1 7 3 97 11 0.8 1 5 6 98 6 0.8 1 5 1 99 8 0.8 1 7 1 100 10 0.8 1 8 2 101 8 0.8 1 6 2 102 5 0.8 1 5 103 8 0.8 1 7 1 104 8 0.8 1 6 2 105 10 0.8 1 8 2 106 14 0.8 1 12 2 107 12 0.8 1 10 2 108 19 0.8 1 13 6 109 11 0.8 1 8 3 110 8 0.8 1 5 3 111 9 0.8 1 7 2 112 14 0.8 1 13 1 113 15 0.8 1 13 2 114 17 0.8 1 14 3 115 9 0.8 1 8 1 116 7 0.8 1 7 117 17 0.8 1 15 2 118 9 0.8 1 9 119 11 0.8 1 9 2 120 23 0.8 1 21 2 121 25 0.8 1 25 122 20 0.8 1 17 3 123 23 0.8 1 19 4 124 19 0.8 1 18 1 125 25 0.8 1 22 3 126 18 0.8 1 16 2 127 17 0.8 1 14 3 128 33 0.8 1 30 3 129 26 0.8 1 25 1 130 23 0.8 1 20 3 131 26 0.8 1 20 6 132 29 0.8 1 23 6 133 34 0.8 1 26 8 134 39 0.8 1 29 10 135 42 0.8 1 19 23 RUN STATISTICS FOR INPUT FILE: SRR13207059_1.fastq.gz ============================================= 54279403 sequences processed in total