SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207058_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 28). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207058_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1434.024 s (17.291 µs/read; 3.47 M reads/minute). === Summary === Total reads processed: 82,933,633 Reads with adapters: 36,177,192 (43.6%) Reads written (passing filters): 82,933,633 (100.0%) Total basepairs processed: 10,519,950,530 bp Quality-trimmed: 2,898,401 bp (0.0%) Total written (filtered): 10,474,395,688 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 36177192 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.3% C: 10.3% G: 6.2% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 32189614 20733408.2 0 32189614 2 2643851 5183352.1 0 2643851 3 730512 1295838.0 0 730512 4 454107 323959.5 0 454107 5 83925 80989.9 0 83925 6 21891 20247.5 0 21891 7 15266 5061.9 0 15266 8 11574 1265.5 0 11574 9 3140 316.4 0 2517 623 10 20789 79.1 1 19181 1608 11 274 19.8 1 43 231 12 104 4.9 1 32 72 13 11 1.2 1 3 8 14 18 1.2 1 10 8 15 7 1.2 1 4 3 16 21 1.2 1 15 6 17 2 1.2 1 1 1 18 35 1.2 1 24 11 19 52 1.2 1 33 19 20 5 1.2 1 2 3 21 5 1.2 1 3 2 22 10 1.2 1 6 4 23 21 1.2 1 15 6 24 33 1.2 1 19 14 25 7 1.2 1 5 2 26 10 1.2 1 6 4 27 14 1.2 1 10 4 28 4 1.2 1 3 1 29 11 1.2 1 8 3 30 27 1.2 1 21 6 31 19 1.2 1 11 8 32 10 1.2 1 6 4 33 35 1.2 1 22 13 34 12 1.2 1 10 2 35 12 1.2 1 8 4 36 15 1.2 1 10 5 37 16 1.2 1 13 3 38 9 1.2 1 7 2 39 17 1.2 1 12 5 40 12 1.2 1 7 5 41 8 1.2 1 6 2 42 15 1.2 1 8 7 43 19 1.2 1 12 7 44 22 1.2 1 17 5 45 6 1.2 1 6 46 10 1.2 1 6 4 47 5 1.2 1 5 48 26 1.2 1 20 6 49 7 1.2 1 5 2 50 6 1.2 1 3 3 51 17 1.2 1 9 8 52 18 1.2 1 14 4 53 16 1.2 1 13 3 54 7 1.2 1 3 4 55 27 1.2 1 21 6 56 17 1.2 1 10 7 57 8 1.2 1 5 3 58 8 1.2 1 6 2 59 8 1.2 1 6 2 60 7 1.2 1 5 2 61 11 1.2 1 9 2 62 18 1.2 1 15 3 63 6 1.2 1 6 64 11 1.2 1 9 2 65 11 1.2 1 11 66 17 1.2 1 8 9 67 13 1.2 1 9 4 68 7 1.2 1 5 2 69 13 1.2 1 13 70 12 1.2 1 10 2 71 6 1.2 1 6 72 13 1.2 1 10 3 73 18 1.2 1 15 3 74 6 1.2 1 6 75 15 1.2 1 11 4 76 8 1.2 1 7 1 77 10 1.2 1 7 3 78 12 1.2 1 9 3 79 14 1.2 1 12 2 80 9 1.2 1 8 1 81 11 1.2 1 11 82 11 1.2 1 9 2 83 13 1.2 1 11 2 84 12 1.2 1 12 85 7 1.2 1 6 1 86 4 1.2 1 4 87 9 1.2 1 6 3 88 10 1.2 1 8 2 89 13 1.2 1 9 4 90 15 1.2 1 14 1 91 21 1.2 1 18 3 92 13 1.2 1 12 1 93 17 1.2 1 16 1 94 23 1.2 1 21 2 95 10 1.2 1 9 1 96 9 1.2 1 9 97 12 1.2 1 12 98 15 1.2 1 12 3 99 18 1.2 1 16 2 100 13 1.2 1 9 4 101 12 1.2 1 10 2 102 14 1.2 1 12 2 103 15 1.2 1 13 2 104 11 1.2 1 9 2 105 16 1.2 1 13 3 106 12 1.2 1 9 3 107 19 1.2 1 17 2 108 22 1.2 1 19 3 109 23 1.2 1 20 3 110 14 1.2 1 13 1 111 31 1.2 1 24 7 112 16 1.2 1 11 5 113 22 1.2 1 19 3 114 16 1.2 1 13 3 115 20 1.2 1 18 2 116 27 1.2 1 25 2 117 14 1.2 1 13 1 118 27 1.2 1 25 2 119 28 1.2 1 22 6 120 38 1.2 1 35 3 121 22 1.2 1 21 1 122 30 1.2 1 26 4 123 25 1.2 1 23 2 124 26 1.2 1 23 3 125 40 1.2 1 33 7 126 25 1.2 1 23 2 127 25 1.2 1 22 3 128 24 1.2 1 21 3 129 27 1.2 1 23 4 130 47 1.2 1 41 6 131 53 1.2 1 46 7 132 45 1.2 1 38 7 133 67 1.2 1 52 15 134 46 1.2 1 25 21 135 53 1.2 1 30 23 RUN STATISTICS FOR INPUT FILE: SRR13207058_1.fastq.gz ============================================= 82933633 sequences processed in total