SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207057_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 25). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207057_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 766.187 s (15.604 µs/read; 3.85 M reads/minute). === Summary === Total reads processed: 49,101,606 Reads with adapters: 20,209,348 (41.2%) Reads written (passing filters): 49,101,606 (100.0%) Total basepairs processed: 6,079,740,279 bp Quality-trimmed: 1,919,505 bp (0.0%) Total written (filtered): 6,053,388,613 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 20209348 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 44.8% C: 10.1% G: 6.8% T: 38.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 17670933 12275401.5 0 17670933 2 1676774 3068850.4 0 1676774 3 435504 767212.6 0 435504 4 294070 191803.1 0 294070 5 71211 47950.8 0 71211 6 17963 11987.7 0 17963 7 13193 2996.9 0 13193 8 9913 749.2 0 9913 9 2386 187.3 0 1891 495 10 15816 46.8 1 14684 1132 11 180 11.7 1 37 143 12 79 2.9 1 24 55 13 11 0.7 1 2 9 14 21 0.7 1 9 12 15 3 0.7 1 1 2 16 16 0.7 1 9 7 17 2 0.7 1 0 2 18 28 0.7 1 13 15 19 35 0.7 1 25 10 20 2 0.7 1 1 1 21 3 0.7 1 3 22 7 0.7 1 5 2 23 13 0.7 1 10 3 24 18 0.7 1 11 7 25 1 0.7 1 1 26 12 0.7 1 8 4 27 14 0.7 1 8 6 28 8 0.7 1 5 3 29 7 0.7 1 7 30 16 0.7 1 11 5 31 18 0.7 1 11 7 32 3 0.7 1 1 2 33 19 0.7 1 15 4 34 3 0.7 1 3 35 14 0.7 1 11 3 36 8 0.7 1 4 4 37 15 0.7 1 12 3 38 7 0.7 1 5 2 39 9 0.7 1 7 2 40 11 0.7 1 8 3 41 15 0.7 1 12 3 42 12 0.7 1 9 3 43 10 0.7 1 8 2 44 11 0.7 1 9 2 45 2 0.7 1 1 1 46 2 0.7 1 0 2 47 5 0.7 1 2 3 48 14 0.7 1 9 5 49 7 0.7 1 4 3 50 8 0.7 1 7 1 51 14 0.7 1 9 5 52 11 0.7 1 8 3 53 5 0.7 1 4 1 54 2 0.7 1 2 55 12 0.7 1 10 2 56 15 0.7 1 12 3 57 4 0.7 1 3 1 58 4 0.7 1 3 1 59 6 0.7 1 5 1 60 4 0.7 1 3 1 61 8 0.7 1 4 4 62 9 0.7 1 7 2 63 3 0.7 1 3 64 5 0.7 1 4 1 65 6 0.7 1 5 1 66 17 0.7 1 14 3 67 4 0.7 1 4 68 9 0.7 1 9 69 6 0.7 1 6 70 6 0.7 1 5 1 71 4 0.7 1 4 72 8 0.7 1 5 3 73 4 0.7 1 4 74 8 0.7 1 8 75 4 0.7 1 4 76 9 0.7 1 7 2 77 11 0.7 1 9 2 78 4 0.7 1 3 1 79 7 0.7 1 5 2 80 9 0.7 1 7 2 81 5 0.7 1 5 82 8 0.7 1 5 3 83 7 0.7 1 6 1 84 6 0.7 1 2 4 85 4 0.7 1 3 1 86 7 0.7 1 7 87 4 0.7 1 4 88 7 0.7 1 7 89 4 0.7 1 2 2 90 4 0.7 1 3 1 91 7 0.7 1 5 2 92 12 0.7 1 8 4 93 10 0.7 1 5 5 94 10 0.7 1 10 95 2 0.7 1 2 96 11 0.7 1 11 97 10 0.7 1 8 2 98 6 0.7 1 3 3 99 10 0.7 1 10 100 14 0.7 1 13 1 101 4 0.7 1 4 102 13 0.7 1 9 4 103 9 0.7 1 8 1 104 8 0.7 1 6 2 105 11 0.7 1 8 3 106 14 0.7 1 14 107 7 0.7 1 6 1 108 13 0.7 1 12 1 109 7 0.7 1 4 3 110 9 0.7 1 7 2 111 14 0.7 1 14 112 11 0.7 1 10 1 113 11 0.7 1 11 114 9 0.7 1 8 1 115 11 0.7 1 8 3 116 13 0.7 1 10 3 117 13 0.7 1 11 2 118 12 0.7 1 12 119 13 0.7 1 12 1 120 17 0.7 1 15 2 121 17 0.7 1 15 2 122 19 0.7 1 11 8 123 16 0.7 1 13 3 124 34 0.7 1 33 1 125 14 0.7 1 10 4 126 19 0.7 1 12 7 127 22 0.7 1 19 3 128 22 0.7 1 17 5 129 20 0.7 1 14 6 130 24 0.7 1 23 1 131 18 0.7 1 17 1 132 24 0.7 1 19 5 133 24 0.7 1 23 1 134 25 0.7 1 18 7 135 33 0.7 1 8 25 RUN STATISTICS FOR INPUT FILE: SRR13207057_1.fastq.gz ============================================= 49101606 sequences processed in total