SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207056_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 10). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207056_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 873.920 s (16.143 µs/read; 3.72 M reads/minute). === Summary === Total reads processed: 54,135,312 Reads with adapters: 23,721,761 (43.8%) Reads written (passing filters): 54,135,312 (100.0%) Total basepairs processed: 6,877,739,495 bp Quality-trimmed: 2,137,120 bp (0.0%) Total written (filtered): 6,847,681,048 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 23721761 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.2% C: 10.4% G: 6.3% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 21118975 13533828.0 0 21118975 2 1726136 3383457.0 0 1726136 3 485217 845864.2 0 485217 4 294326 211466.1 0 294326 5 50975 52866.5 0 50975 6 13477 13216.6 0 13477 7 9619 3304.2 0 9619 8 7050 826.0 0 7050 9 1973 206.5 0 1544 429 10 12595 51.6 1 11537 1058 11 162 12.9 1 26 136 12 46 3.2 1 15 31 13 5 0.8 1 3 2 14 10 0.8 1 6 4 15 4 0.8 1 1 3 16 11 0.8 1 6 5 17 2 0.8 1 2 18 15 0.8 1 9 6 19 13 0.8 1 6 7 20 3 0.8 1 2 1 21 9 0.8 1 7 2 22 3 0.8 1 1 2 23 13 0.8 1 10 3 24 9 0.8 1 4 5 25 3 0.8 1 2 1 26 5 0.8 1 2 3 27 3 0.8 1 3 28 5 0.8 1 3 2 29 6 0.8 1 5 1 30 9 0.8 1 4 5 31 12 0.8 1 7 5 32 4 0.8 1 2 2 33 4 0.8 1 2 2 34 2 0.8 1 2 35 6 0.8 1 3 3 36 9 0.8 1 6 3 37 3 0.8 1 3 38 7 0.8 1 2 5 39 9 0.8 1 9 40 7 0.8 1 5 2 41 5 0.8 1 3 2 42 3 0.8 1 3 43 6 0.8 1 4 2 44 6 0.8 1 3 3 45 6 0.8 1 2 4 46 5 0.8 1 2 3 47 5 0.8 1 3 2 48 4 0.8 1 3 1 49 4 0.8 1 4 50 6 0.8 1 5 1 51 3 0.8 1 3 52 10 0.8 1 9 1 53 3 0.8 1 2 1 54 7 0.8 1 5 2 55 5 0.8 1 3 2 56 8 0.8 1 6 2 57 6 0.8 1 5 1 58 3 0.8 1 3 59 6 0.8 1 4 2 60 3 0.8 1 2 1 61 8 0.8 1 5 3 62 5 0.8 1 4 1 63 2 0.8 1 1 1 64 5 0.8 1 3 2 65 4 0.8 1 3 1 66 6 0.8 1 4 2 67 6 0.8 1 4 2 68 3 0.8 1 2 1 69 6 0.8 1 5 1 70 8 0.8 1 7 1 71 4 0.8 1 2 2 72 5 0.8 1 5 73 5 0.8 1 4 1 74 5 0.8 1 4 1 75 6 0.8 1 6 76 1 0.8 1 1 77 5 0.8 1 3 2 78 5 0.8 1 4 1 79 1 0.8 1 0 1 80 5 0.8 1 4 1 81 10 0.8 1 7 3 82 2 0.8 1 2 83 6 0.8 1 5 1 84 8 0.8 1 7 1 85 7 0.8 1 7 86 9 0.8 1 7 2 87 10 0.8 1 8 2 88 14 0.8 1 9 5 89 9 0.8 1 7 2 90 15 0.8 1 11 4 91 11 0.8 1 5 6 92 9 0.8 1 8 1 93 5 0.8 1 5 94 13 0.8 1 12 1 95 13 0.8 1 11 2 96 4 0.8 1 4 97 6 0.8 1 2 4 98 6 0.8 1 3 3 99 13 0.8 1 11 2 100 8 0.8 1 8 101 12 0.8 1 11 1 102 8 0.8 1 7 1 103 11 0.8 1 6 5 104 6 0.8 1 5 1 105 12 0.8 1 12 106 15 0.8 1 15 107 7 0.8 1 6 1 108 13 0.8 1 13 109 8 0.8 1 8 110 14 0.8 1 13 1 111 12 0.8 1 11 1 112 17 0.8 1 15 2 113 15 0.8 1 13 2 114 8 0.8 1 8 115 10 0.8 1 9 1 116 9 0.8 1 8 1 117 10 0.8 1 9 1 118 28 0.8 1 24 4 119 13 0.8 1 12 1 120 22 0.8 1 16 6 121 10 0.8 1 6 4 122 25 0.8 1 21 4 123 25 0.8 1 18 7 124 16 0.8 1 14 2 125 19 0.8 1 18 1 126 13 0.8 1 12 1 127 14 0.8 1 13 1 128 28 0.8 1 27 1 129 29 0.8 1 25 4 130 38 0.8 1 33 5 131 22 0.8 1 17 5 132 32 0.8 1 30 2 133 28 0.8 1 22 6 134 34 0.8 1 23 11 135 59 0.8 1 22 37 RUN STATISTICS FOR INPUT FILE: SRR13207056_1.fastq.gz ============================================= 54135312 sequences processed in total