SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207055_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 18). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207055_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 632.569 s (17.075 µs/read; 3.51 M reads/minute). === Summary === Total reads processed: 37,045,803 Reads with adapters: 16,116,353 (43.5%) Reads written (passing filters): 37,045,803 (100.0%) Total basepairs processed: 4,679,181,409 bp Quality-trimmed: 2,133,036 bp (0.0%) Total written (filtered): 4,657,981,585 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 16116353 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 44.6% C: 10.7% G: 6.5% T: 38.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 14285260 9261450.8 0 14285260 2 1214770 2315362.7 0 1214770 3 342924 578840.7 0 342924 4 202583 144710.2 0 202583 5 37095 36177.5 0 37095 6 9891 9044.4 0 9891 7 7025 2261.1 0 7025 8 5226 565.3 0 5226 9 1458 141.3 0 1143 315 10 9219 35.3 1 8384 835 11 98 8.8 1 13 85 12 36 2.2 1 3 33 13 11 0.6 1 2 9 14 7 0.6 1 4 3 15 3 0.6 1 0 3 16 9 0.6 1 7 2 17 2 0.6 1 2 18 6 0.6 1 3 3 19 7 0.6 1 2 5 20 1 0.6 1 1 21 4 0.6 1 2 2 22 1 0.6 1 1 23 8 0.6 1 6 2 24 4 0.6 1 2 2 25 2 0.6 1 2 26 8 0.6 1 6 2 27 2 0.6 1 0 2 30 2 0.6 1 2 31 3 0.6 1 1 2 32 2 0.6 1 1 1 33 6 0.6 1 2 4 34 3 0.6 1 2 1 35 3 0.6 1 2 1 36 5 0.6 1 4 1 37 8 0.6 1 2 6 38 5 0.6 1 4 1 39 6 0.6 1 6 40 4 0.6 1 4 41 1 0.6 1 1 42 7 0.6 1 3 4 43 4 0.6 1 2 2 44 4 0.6 1 1 3 45 1 0.6 1 1 46 3 0.6 1 1 2 47 2 0.6 1 2 48 1 0.6 1 1 49 1 0.6 1 1 50 6 0.6 1 4 2 51 3 0.6 1 2 1 52 3 0.6 1 1 2 53 1 0.6 1 1 54 3 0.6 1 3 55 2 0.6 1 0 2 56 3 0.6 1 3 57 3 0.6 1 3 58 3 0.6 1 3 59 1 0.6 1 1 61 3 0.6 1 1 2 62 5 0.6 1 5 63 7 0.6 1 5 2 64 4 0.6 1 3 1 65 5 0.6 1 4 1 66 5 0.6 1 4 1 67 6 0.6 1 5 1 68 5 0.6 1 4 1 69 8 0.6 1 4 4 70 2 0.6 1 2 71 2 0.6 1 2 72 3 0.6 1 2 1 73 5 0.6 1 2 3 74 4 0.6 1 4 75 7 0.6 1 5 2 76 6 0.6 1 6 77 3 0.6 1 2 1 78 7 0.6 1 7 79 3 0.6 1 2 1 80 5 0.6 1 5 81 4 0.6 1 3 1 82 5 0.6 1 5 83 5 0.6 1 2 3 84 4 0.6 1 3 1 85 5 0.6 1 4 1 86 2 0.6 1 2 87 7 0.6 1 6 1 88 3 0.6 1 2 1 89 6 0.6 1 5 1 90 5 0.6 1 2 3 91 1 0.6 1 1 92 3 0.6 1 2 1 93 8 0.6 1 7 1 94 4 0.6 1 3 1 95 8 0.6 1 6 2 96 9 0.6 1 8 1 97 3 0.6 1 2 1 98 5 0.6 1 4 1 99 6 0.6 1 4 2 100 7 0.6 1 6 1 101 6 0.6 1 5 1 102 5 0.6 1 3 2 103 7 0.6 1 7 104 2 0.6 1 1 1 105 3 0.6 1 3 106 7 0.6 1 6 1 107 10 0.6 1 10 108 10 0.6 1 7 3 109 3 0.6 1 3 110 2 0.6 1 2 111 6 0.6 1 4 2 112 10 0.6 1 8 2 113 12 0.6 1 11 1 114 13 0.6 1 9 4 115 9 0.6 1 7 2 116 10 0.6 1 10 117 4 0.6 1 3 1 118 13 0.6 1 12 1 119 17 0.6 1 15 2 120 15 0.6 1 14 1 121 6 0.6 1 5 1 122 14 0.6 1 12 2 123 16 0.6 1 14 2 124 9 0.6 1 6 3 125 9 0.6 1 9 126 10 0.6 1 10 127 20 0.6 1 20 128 13 0.6 1 11 2 129 17 0.6 1 17 130 20 0.6 1 20 131 19 0.6 1 15 4 132 24 0.6 1 21 3 133 21 0.6 1 16 5 134 16 0.6 1 14 2 135 21 0.6 1 11 10 RUN STATISTICS FOR INPUT FILE: SRR13207055_1.fastq.gz ============================================= 37045803 sequences processed in total