SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207054_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 22). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207054_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 971.391 s (16.351 µs/read; 3.67 M reads/minute). === Summary === Total reads processed: 59,408,352 Reads with adapters: 25,824,093 (43.5%) Reads written (passing filters): 59,408,352 (100.0%) Total basepairs processed: 7,517,784,280 bp Quality-trimmed: 2,588,934 bp (0.0%) Total written (filtered): 7,484,679,127 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 25824093 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.1% C: 10.4% G: 6.3% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 22938238 14852088.0 0 22938238 2 1912707 3713022.0 0 1912707 3 532313 928255.5 0 532313 4 327693 232063.9 0 327693 5 60219 58016.0 0 60219 6 15425 14504.0 0 15425 7 11062 3626.0 0 11062 8 8148 906.5 0 8148 9 2048 226.6 0 1595 453 10 14157 56.7 1 12921 1236 11 179 14.2 1 41 138 12 82 3.5 1 30 52 13 7 0.9 1 1 6 14 28 0.9 1 18 10 15 6 0.9 1 5 1 16 19 0.9 1 13 6 17 3 0.9 1 1 2 18 40 0.9 1 23 17 19 41 0.9 1 30 11 20 7 0.9 1 4 3 21 2 0.9 1 2 22 9 0.9 1 4 5 23 21 0.9 1 15 6 24 24 0.9 1 19 5 25 5 0.9 1 5 26 16 0.9 1 9 7 27 17 0.9 1 14 3 28 2 0.9 1 1 1 29 8 0.9 1 7 1 30 18 0.9 1 10 8 31 27 0.9 1 15 12 32 5 0.9 1 5 33 17 0.9 1 11 6 34 12 0.9 1 8 4 35 15 0.9 1 11 4 36 16 0.9 1 11 5 37 26 0.9 1 19 7 38 8 0.9 1 4 4 39 9 0.9 1 6 3 40 12 0.9 1 8 4 41 16 0.9 1 9 7 42 14 0.9 1 7 7 43 23 0.9 1 18 5 44 21 0.9 1 14 7 45 7 0.9 1 5 2 46 10 0.9 1 7 3 47 12 0.9 1 9 3 48 14 0.9 1 11 3 49 16 0.9 1 13 3 50 8 0.9 1 4 4 51 14 0.9 1 10 4 52 35 0.9 1 20 15 53 5 0.9 1 3 2 54 3 0.9 1 3 55 26 0.9 1 18 8 56 22 0.9 1 17 5 57 4 0.9 1 4 58 8 0.9 1 5 3 59 11 0.9 1 10 1 60 2 0.9 1 1 1 61 5 0.9 1 4 1 62 9 0.9 1 7 2 63 6 0.9 1 5 1 64 4 0.9 1 3 1 65 6 0.9 1 6 66 19 0.9 1 15 4 67 10 0.9 1 9 1 68 8 0.9 1 8 69 14 0.9 1 10 4 70 8 0.9 1 6 2 71 11 0.9 1 11 72 11 0.9 1 9 2 73 10 0.9 1 9 1 74 15 0.9 1 14 1 75 6 0.9 1 6 76 16 0.9 1 15 1 77 15 0.9 1 11 4 78 12 0.9 1 10 2 79 8 0.9 1 6 2 80 10 0.9 1 8 2 81 13 0.9 1 9 4 82 10 0.9 1 8 2 83 6 0.9 1 6 84 4 0.9 1 4 85 16 0.9 1 12 4 86 7 0.9 1 5 2 87 10 0.9 1 8 2 88 13 0.9 1 12 1 89 9 0.9 1 8 1 90 8 0.9 1 5 3 91 12 0.9 1 8 4 92 15 0.9 1 9 6 93 9 0.9 1 8 1 94 13 0.9 1 9 4 95 6 0.9 1 6 96 14 0.9 1 14 97 14 0.9 1 12 2 98 13 0.9 1 9 4 99 13 0.9 1 11 2 100 8 0.9 1 7 1 101 10 0.9 1 8 2 102 9 0.9 1 6 3 103 7 0.9 1 6 1 104 14 0.9 1 13 1 105 11 0.9 1 9 2 106 21 0.9 1 20 1 107 13 0.9 1 11 2 108 12 0.9 1 9 3 109 13 0.9 1 12 1 110 8 0.9 1 7 1 111 10 0.9 1 8 2 112 17 0.9 1 13 4 113 13 0.9 1 11 2 114 18 0.9 1 16 2 115 9 0.9 1 7 2 116 15 0.9 1 9 6 117 11 0.9 1 11 118 19 0.9 1 17 2 119 22 0.9 1 16 6 120 17 0.9 1 13 4 121 25 0.9 1 24 1 122 29 0.9 1 20 9 123 20 0.9 1 17 3 124 17 0.9 1 16 1 125 18 0.9 1 13 5 126 25 0.9 1 23 2 127 18 0.9 1 15 3 128 26 0.9 1 23 3 129 45 0.9 1 34 11 130 39 0.9 1 32 7 131 30 0.9 1 26 4 132 38 0.9 1 33 5 133 30 0.9 1 24 6 134 40 0.9 1 26 14 135 46 0.9 1 16 30 RUN STATISTICS FOR INPUT FILE: SRR13207054_1.fastq.gz ============================================= 59408352 sequences processed in total