Bismark report for: SRR13207053_val_1.fq.gz and SRR13207053_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 49606884 Number of paired-end alignments with a unique best hit: 14854553 Mapping efficiency: 29.9% Sequence pairs with no alignments under any condition: 31209535 Sequence pairs did not map uniquely: 3542796 Sequence pairs which were discarded because genomic sequence could not be extracted: 10 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7422076 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 7432467 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 617828482 Total methylated C's in CpG context: 8615943 Total methylated C's in CHG context: 1006114 Total methylated C's in CHH context: 7260438 Total methylated C's in Unknown context: 31367 Total unmethylated C's in CpG context: 69650536 Total unmethylated C's in CHG context: 106524516 Total unmethylated C's in CHH context: 424770935 Total unmethylated C's in Unknown context: 1573371 C methylated in CpG context: 11.0% C methylated in CHG context: 0.9% C methylated in CHH context: 1.7% C methylated in Unknown context (CN or CHN): 2.0% Bismark completed in 0d 1h 38m 19s