SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207053_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 14). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207053_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 814.706 s (16.422 µs/read; 3.65 M reads/minute). === Summary === Total reads processed: 49,611,452 Reads with adapters: 21,362,959 (43.1%) Reads written (passing filters): 49,611,452 (100.0%) Total basepairs processed: 6,252,863,959 bp Quality-trimmed: 1,776,907 bp (0.0%) Total written (filtered): 6,225,809,755 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 21362959 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.2% C: 10.4% G: 6.4% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 18933491 12402863.0 0 18933491 2 1613051 3100715.8 0 1613051 3 445071 775178.9 0 445071 4 275236 193794.7 0 275236 5 51936 48448.7 0 51936 6 13128 12112.2 0 13128 7 9175 3028.0 0 9175 8 6901 757.0 0 6901 9 1821 189.3 0 1428 393 10 11758 47.3 1 10773 985 11 162 11.8 1 27 135 12 60 3.0 1 22 38 13 7 0.7 1 2 5 14 23 0.7 1 13 10 15 5 0.7 1 3 2 16 20 0.7 1 17 3 18 38 0.7 1 22 16 19 30 0.7 1 19 11 20 2 0.7 1 1 1 21 1 0.7 1 1 22 1 0.7 1 1 23 24 0.7 1 14 10 24 19 0.7 1 14 5 25 3 0.7 1 2 1 26 8 0.7 1 5 3 27 5 0.7 1 5 28 1 0.7 1 0 1 29 8 0.7 1 4 4 30 15 0.7 1 10 5 31 21 0.7 1 15 6 32 4 0.7 1 3 1 33 15 0.7 1 10 5 34 7 0.7 1 1 6 35 10 0.7 1 6 4 36 10 0.7 1 7 3 37 8 0.7 1 4 4 38 19 0.7 1 9 10 39 16 0.7 1 10 6 40 5 0.7 1 4 1 41 14 0.7 1 9 5 42 9 0.7 1 7 2 43 9 0.7 1 4 5 44 18 0.7 1 13 5 45 3 0.7 1 3 46 12 0.7 1 8 4 47 5 0.7 1 5 48 21 0.7 1 16 5 49 13 0.7 1 6 7 50 13 0.7 1 6 7 51 11 0.7 1 6 5 52 17 0.7 1 14 3 53 5 0.7 1 5 54 5 0.7 1 5 55 20 0.7 1 13 7 56 11 0.7 1 8 3 57 1 0.7 1 1 58 5 0.7 1 4 1 59 9 0.7 1 6 3 60 1 0.7 1 1 61 2 0.7 1 2 62 11 0.7 1 7 4 63 2 0.7 1 2 64 7 0.7 1 5 2 65 3 0.7 1 0 3 66 11 0.7 1 8 3 67 5 0.7 1 4 1 68 8 0.7 1 6 2 69 8 0.7 1 6 2 70 3 0.7 1 3 71 4 0.7 1 1 3 72 4 0.7 1 3 1 73 6 0.7 1 2 4 74 2 0.7 1 2 75 3 0.7 1 2 1 76 7 0.7 1 5 2 77 13 0.7 1 9 4 78 7 0.7 1 6 1 79 6 0.7 1 3 3 80 8 0.7 1 6 2 81 7 0.7 1 7 82 2 0.7 1 1 1 83 6 0.7 1 4 2 84 1 0.7 1 0 1 85 11 0.7 1 11 86 10 0.7 1 5 5 87 4 0.7 1 4 88 9 0.7 1 6 3 89 7 0.7 1 7 90 6 0.7 1 4 2 91 7 0.7 1 5 2 92 2 0.7 1 2 93 5 0.7 1 5 94 1 0.7 1 1 95 4 0.7 1 4 96 7 0.7 1 7 97 3 0.7 1 2 1 98 8 0.7 1 8 99 10 0.7 1 9 1 100 5 0.7 1 4 1 101 4 0.7 1 3 1 102 7 0.7 1 7 103 5 0.7 1 3 2 104 7 0.7 1 6 1 105 8 0.7 1 5 3 106 8 0.7 1 5 3 107 7 0.7 1 5 2 108 4 0.7 1 3 1 109 4 0.7 1 3 1 110 5 0.7 1 3 2 111 10 0.7 1 10 112 5 0.7 1 4 1 113 5 0.7 1 5 114 6 0.7 1 6 115 7 0.7 1 6 1 116 7 0.7 1 5 2 117 13 0.7 1 12 1 118 19 0.7 1 16 3 119 12 0.7 1 11 1 120 8 0.7 1 8 121 13 0.7 1 12 1 122 13 0.7 1 10 3 123 13 0.7 1 13 124 12 0.7 1 12 125 20 0.7 1 13 7 126 11 0.7 1 5 6 127 10 0.7 1 8 2 128 18 0.7 1 18 129 14 0.7 1 10 4 130 15 0.7 1 13 2 131 23 0.7 1 19 4 132 22 0.7 1 17 5 133 17 0.7 1 13 4 134 24 0.7 1 14 10 135 31 0.7 1 11 20 RUN STATISTICS FOR INPUT FILE: SRR13207053_1.fastq.gz ============================================= 49611452 sequences processed in total