SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207052_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 16). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207052_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 881.775 s (16.986 µs/read; 3.53 M reads/minute). === Summary === Total reads processed: 51,910,846 Reads with adapters: 21,719,554 (41.8%) Reads written (passing filters): 51,910,846 (100.0%) Total basepairs processed: 6,471,217,372 bp Quality-trimmed: 2,317,730 bp (0.0%) Total written (filtered): 6,442,864,567 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 21719554 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 44.8% C: 10.3% G: 6.6% T: 38.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 19088005 12977711.5 0 19088005 2 1742115 3244427.9 0 1742115 3 463014 811107.0 0 463014 4 301783 202776.7 0 301783 5 65986 50694.2 0 65986 6 16831 12673.5 0 16831 7 12600 3168.4 0 12600 8 9587 792.1 0 9587 9 2503 198.0 0 2004 499 10 16022 49.5 1 14806 1216 11 172 12.4 1 26 146 12 50 3.1 1 11 39 13 4 0.8 1 0 4 14 3 0.8 1 2 1 15 1 0.8 1 0 1 16 5 0.8 1 2 3 17 3 0.8 1 2 1 18 12 0.8 1 6 6 19 6 0.8 1 3 3 20 2 0.8 1 0 2 21 3 0.8 1 0 3 22 1 0.8 1 0 1 23 3 0.8 1 3 24 2 0.8 1 2 25 1 0.8 1 0 1 26 1 0.8 1 1 27 4 0.8 1 3 1 28 4 0.8 1 3 1 29 5 0.8 1 5 31 2 0.8 1 1 1 33 1 0.8 1 1 34 1 0.8 1 0 1 35 7 0.8 1 3 4 36 2 0.8 1 1 1 37 2 0.8 1 0 2 38 3 0.8 1 2 1 39 5 0.8 1 2 3 40 3 0.8 1 2 1 42 4 0.8 1 1 3 43 2 0.8 1 1 1 44 4 0.8 1 4 45 3 0.8 1 2 1 46 5 0.8 1 3 2 48 7 0.8 1 4 3 49 3 0.8 1 1 2 50 1 0.8 1 1 51 5 0.8 1 3 2 52 3 0.8 1 1 2 53 1 0.8 1 1 54 5 0.8 1 2 3 55 2 0.8 1 1 1 56 1 0.8 1 1 57 2 0.8 1 0 2 58 4 0.8 1 4 59 9 0.8 1 9 60 5 0.8 1 3 2 61 7 0.8 1 3 4 62 6 0.8 1 3 3 63 2 0.8 1 1 1 64 1 0.8 1 0 1 65 6 0.8 1 6 66 6 0.8 1 3 3 67 3 0.8 1 3 68 7 0.8 1 7 69 10 0.8 1 8 2 70 5 0.8 1 4 1 71 6 0.8 1 5 1 72 10 0.8 1 7 3 73 7 0.8 1 6 1 74 5 0.8 1 3 2 75 4 0.8 1 4 76 4 0.8 1 4 77 2 0.8 1 1 1 78 3 0.8 1 2 1 79 5 0.8 1 4 1 80 2 0.8 1 2 81 2 0.8 1 2 82 9 0.8 1 7 2 83 5 0.8 1 5 84 9 0.8 1 7 2 85 3 0.8 1 2 1 86 8 0.8 1 7 1 87 1 0.8 1 1 88 6 0.8 1 5 1 89 4 0.8 1 3 1 90 7 0.8 1 6 1 91 9 0.8 1 8 1 92 8 0.8 1 4 4 93 4 0.8 1 3 1 94 10 0.8 1 9 1 95 7 0.8 1 6 1 96 8 0.8 1 6 2 97 10 0.8 1 8 2 98 16 0.8 1 10 6 99 4 0.8 1 2 2 100 5 0.8 1 4 1 101 9 0.8 1 7 2 102 8 0.8 1 5 3 103 8 0.8 1 8 104 5 0.8 1 4 1 105 4 0.8 1 4 106 10 0.8 1 6 4 107 4 0.8 1 3 1 108 18 0.8 1 12 6 109 15 0.8 1 15 110 14 0.8 1 14 111 15 0.8 1 14 1 112 12 0.8 1 11 1 113 12 0.8 1 12 114 10 0.8 1 8 2 115 6 0.8 1 5 1 116 16 0.8 1 11 5 117 19 0.8 1 17 2 118 9 0.8 1 7 2 119 5 0.8 1 3 2 120 7 0.8 1 6 1 121 15 0.8 1 13 2 122 11 0.8 1 10 1 123 12 0.8 1 10 2 124 12 0.8 1 10 2 125 12 0.8 1 11 1 126 30 0.8 1 27 3 127 14 0.8 1 14 128 14 0.8 1 14 129 17 0.8 1 17 130 15 0.8 1 13 2 131 26 0.8 1 21 5 132 28 0.8 1 23 5 133 25 0.8 1 19 6 134 25 0.8 1 19 6 135 36 0.8 1 14 22 RUN STATISTICS FOR INPUT FILE: SRR13207052_1.fastq.gz ============================================= 51910846 sequences processed in total