SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207051_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 25). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207051_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 938.114 s (16.005 µs/read; 3.75 M reads/minute). === Summary === Total reads processed: 58,612,409 Reads with adapters: 24,942,134 (42.6%) Reads written (passing filters): 58,612,409 (100.0%) Total basepairs processed: 7,367,240,322 bp Quality-trimmed: 2,008,699 bp (0.0%) Total written (filtered): 7,335,778,618 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 24942134 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.4% C: 10.2% G: 6.4% T: 38.0% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 22192488 14653102.2 0 22192488 2 1784824 3663275.6 0 1784824 3 509781 915818.9 0 509781 4 336912 228954.7 0 336912 5 64715 57238.7 0 64715 6 15565 14309.7 0 15565 7 11496 3577.4 0 11496 8 8910 894.4 0 8910 9 2289 223.6 0 1884 405 10 14019 55.9 1 12878 1141 11 169 14.0 1 18 151 12 47 3.5 1 13 34 13 5 0.9 1 1 4 14 8 0.9 1 5 3 15 4 0.9 1 1 3 16 3 0.9 1 1 2 17 13 0.9 1 9 4 18 5 0.9 1 3 2 19 8 0.9 1 4 4 20 4 0.9 1 0 4 21 7 0.9 1 5 2 22 6 0.9 1 3 3 23 4 0.9 1 3 1 24 14 0.9 1 6 8 25 5 0.9 1 3 2 26 4 0.9 1 2 2 27 3 0.9 1 1 2 28 4 0.9 1 2 2 29 2 0.9 1 1 1 30 6 0.9 1 4 2 31 1 0.9 1 1 32 10 0.9 1 5 5 33 13 0.9 1 6 7 34 3 0.9 1 1 2 35 7 0.9 1 3 4 36 2 0.9 1 2 38 7 0.9 1 6 1 39 4 0.9 1 1 3 40 2 0.9 1 0 2 42 4 0.9 1 1 3 43 3 0.9 1 2 1 44 3 0.9 1 1 2 45 6 0.9 1 4 2 46 6 0.9 1 2 4 47 5 0.9 1 4 1 48 6 0.9 1 4 2 49 6 0.9 1 3 3 50 2 0.9 1 2 51 2 0.9 1 1 1 52 4 0.9 1 3 1 53 10 0.9 1 7 3 54 1 0.9 1 1 55 3 0.9 1 3 56 5 0.9 1 5 57 7 0.9 1 3 4 58 3 0.9 1 1 2 59 4 0.9 1 3 1 60 12 0.9 1 5 7 61 4 0.9 1 4 62 3 0.9 1 3 63 10 0.9 1 7 3 64 5 0.9 1 4 1 65 1 0.9 1 0 1 66 7 0.9 1 6 1 67 3 0.9 1 3 68 3 0.9 1 3 69 4 0.9 1 4 70 6 0.9 1 6 71 4 0.9 1 3 1 72 3 0.9 1 2 1 73 5 0.9 1 2 3 74 4 0.9 1 3 1 75 3 0.9 1 2 1 76 6 0.9 1 3 3 77 7 0.9 1 4 3 78 5 0.9 1 4 1 79 6 0.9 1 5 1 80 5 0.9 1 5 81 4 0.9 1 3 1 82 2 0.9 1 0 2 83 6 0.9 1 2 4 84 8 0.9 1 8 85 7 0.9 1 5 2 86 3 0.9 1 3 87 7 0.9 1 5 2 88 5 0.9 1 3 2 89 9 0.9 1 4 5 90 7 0.9 1 5 2 91 11 0.9 1 8 3 92 9 0.9 1 9 93 6 0.9 1 5 1 94 5 0.9 1 4 1 95 4 0.9 1 2 2 96 9 0.9 1 9 97 12 0.9 1 9 3 98 3 0.9 1 3 99 6 0.9 1 5 1 100 8 0.9 1 7 1 101 9 0.9 1 8 1 102 5 0.9 1 4 1 103 3 0.9 1 3 104 5 0.9 1 5 105 6 0.9 1 4 2 106 6 0.9 1 5 1 107 8 0.9 1 5 3 108 13 0.9 1 11 2 109 13 0.9 1 12 1 110 5 0.9 1 5 111 11 0.9 1 11 112 8 0.9 1 5 3 113 6 0.9 1 6 114 9 0.9 1 6 3 115 15 0.9 1 13 2 116 8 0.9 1 8 117 17 0.9 1 12 5 118 10 0.9 1 8 2 119 10 0.9 1 7 3 120 21 0.9 1 18 3 121 8 0.9 1 8 122 10 0.9 1 9 1 123 12 0.9 1 11 1 124 9 0.9 1 8 1 125 17 0.9 1 15 2 126 10 0.9 1 8 2 127 27 0.9 1 26 1 128 20 0.9 1 19 1 129 17 0.9 1 14 3 130 16 0.9 1 14 2 131 12 0.9 1 10 2 132 38 0.9 1 32 6 133 15 0.9 1 11 4 134 25 0.9 1 17 8 135 24 0.9 1 11 13 RUN STATISTICS FOR INPUT FILE: SRR13207051_2.fastq.gz ============================================= 58612409 sequences processed in total Total number of sequences analysed for the sequence pair length validation: 58612409 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 5087 (0.01%)