SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207051_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 25). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207051_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 937.611 s (15.997 µs/read; 3.75 M reads/minute). === Summary === Total reads processed: 58,612,409 Reads with adapters: 25,043,792 (42.7%) Reads written (passing filters): 58,612,409 (100.0%) Total basepairs processed: 7,368,913,020 bp Quality-trimmed: 1,953,416 bp (0.0%) Total written (filtered): 7,337,218,200 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 25043792 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.1% C: 10.4% G: 6.5% T: 37.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 22152651 14653102.2 0 22152651 2 1919505 3663275.6 0 1919505 3 518513 915818.9 0 518513 4 329859 228954.7 0 329859 5 66332 57238.7 0 66332 6 16516 14309.7 0 16516 7 12191 3577.4 0 12191 8 9163 894.4 0 9163 9 2338 223.6 0 1847 491 10 14730 55.9 1 13538 1192 11 197 14.0 1 33 164 12 109 3.5 1 50 59 13 7 0.9 1 3 4 14 36 0.9 1 22 14 15 4 0.9 1 1 3 16 30 0.9 1 20 10 17 2 0.9 1 1 1 18 52 0.9 1 32 20 19 66 0.9 1 43 23 20 5 0.9 1 2 3 21 6 0.9 1 3 3 22 5 0.9 1 2 3 23 36 0.9 1 25 11 24 51 0.9 1 35 16 25 2 0.9 1 2 26 26 0.9 1 19 7 27 21 0.9 1 16 5 28 4 0.9 1 2 2 29 22 0.9 1 11 11 30 32 0.9 1 27 5 31 31 0.9 1 24 7 32 7 0.9 1 5 2 33 29 0.9 1 22 7 34 9 0.9 1 7 2 35 12 0.9 1 7 5 36 18 0.9 1 14 4 37 18 0.9 1 10 8 38 9 0.9 1 6 3 39 15 0.9 1 13 2 40 20 0.9 1 13 7 41 15 0.9 1 13 2 42 24 0.9 1 20 4 43 28 0.9 1 20 8 44 31 0.9 1 27 4 45 12 0.9 1 9 3 46 14 0.9 1 9 5 47 4 0.9 1 2 2 48 34 0.9 1 30 4 49 11 0.9 1 10 1 50 6 0.9 1 4 2 51 13 0.9 1 10 3 52 40 0.9 1 30 10 53 4 0.9 1 3 1 54 9 0.9 1 7 2 55 28 0.9 1 18 10 56 18 0.9 1 11 7 57 5 0.9 1 4 1 58 7 0.9 1 5 2 59 21 0.9 1 18 3 60 2 0.9 1 1 1 61 2 0.9 1 2 62 19 0.9 1 15 4 63 7 0.9 1 7 64 2 0.9 1 2 65 9 0.9 1 9 66 21 0.9 1 16 5 67 3 0.9 1 1 2 68 7 0.9 1 7 69 6 0.9 1 5 1 70 4 0.9 1 1 3 71 5 0.9 1 5 72 9 0.9 1 6 3 73 9 0.9 1 8 1 74 9 0.9 1 8 1 75 6 0.9 1 3 3 76 8 0.9 1 5 3 77 8 0.9 1 7 1 78 8 0.9 1 7 1 79 6 0.9 1 4 2 80 10 0.9 1 8 2 81 8 0.9 1 5 3 82 8 0.9 1 6 2 83 11 0.9 1 10 1 84 8 0.9 1 6 2 85 8 0.9 1 5 3 86 10 0.9 1 5 5 87 6 0.9 1 5 1 88 7 0.9 1 5 2 89 5 0.9 1 5 90 5 0.9 1 4 1 91 11 0.9 1 9 2 92 9 0.9 1 6 3 93 9 0.9 1 8 1 94 8 0.9 1 7 1 95 7 0.9 1 6 1 96 12 0.9 1 8 4 97 9 0.9 1 6 3 98 9 0.9 1 7 2 99 8 0.9 1 6 2 100 6 0.9 1 2 4 101 5 0.9 1 4 1 102 6 0.9 1 4 2 103 1 0.9 1 1 104 14 0.9 1 11 3 105 7 0.9 1 6 1 106 10 0.9 1 7 3 107 7 0.9 1 6 1 108 10 0.9 1 9 1 109 5 0.9 1 4 1 110 8 0.9 1 5 3 111 11 0.9 1 7 4 112 3 0.9 1 2 1 113 5 0.9 1 4 1 114 16 0.9 1 16 115 11 0.9 1 8 3 116 7 0.9 1 6 1 117 9 0.9 1 9 118 20 0.9 1 14 6 119 19 0.9 1 12 7 120 14 0.9 1 14 121 22 0.9 1 19 3 122 19 0.9 1 13 6 123 11 0.9 1 10 1 124 9 0.9 1 8 1 125 6 0.9 1 5 1 126 13 0.9 1 13 127 9 0.9 1 8 1 128 20 0.9 1 18 2 129 19 0.9 1 18 1 130 28 0.9 1 26 2 131 16 0.9 1 15 1 132 21 0.9 1 21 133 24 0.9 1 18 6 134 24 0.9 1 17 7 135 36 0.9 1 12 24 RUN STATISTICS FOR INPUT FILE: SRR13207051_1.fastq.gz ============================================= 58612409 sequences processed in total