SUMMARISING RUN PARAMETERS ========================== Input filename: SRR13207050_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 25). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR13207050_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 841.454 s (16.189 µs/read; 3.71 M reads/minute). === Summary === Total reads processed: 51,977,624 Reads with adapters: 22,254,918 (42.8%) Reads written (passing filters): 51,977,624 (100.0%) Total basepairs processed: 6,538,749,697 bp Quality-trimmed: 1,953,148 bp (0.0%) Total written (filtered): 6,510,376,445 bp (99.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 22254918 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.0% C: 10.4% G: 6.5% T: 38.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 19679963 12994406.0 0 19679963 2 1715586 3248601.5 0 1715586 3 462343 812150.4 0 462343 4 293120 203037.6 0 293120 5 55471 50759.4 0 55471 6 14322 12689.8 0 14322 7 10221 3172.5 0 10221 8 7505 793.1 0 7505 9 1871 198.3 0 1431 440 10 12785 49.6 1 11769 1016 11 155 12.4 1 38 117 12 86 3.1 1 37 49 13 7 0.8 1 0 7 14 32 0.8 1 25 7 15 3 0.8 1 3 16 31 0.8 1 25 6 17 1 0.8 1 0 1 18 38 0.8 1 25 13 19 50 0.8 1 34 16 20 9 0.8 1 7 2 21 1 0.8 1 1 22 5 0.8 1 2 3 23 13 0.8 1 5 8 24 51 0.8 1 39 12 25 4 0.8 1 4 26 21 0.8 1 15 6 27 14 0.8 1 10 4 28 5 0.8 1 5 29 13 0.8 1 7 6 30 26 0.8 1 22 4 31 24 0.8 1 14 10 32 4 0.8 1 2 2 33 25 0.8 1 19 6 34 9 0.8 1 6 3 35 11 0.8 1 6 5 36 20 0.8 1 15 5 37 17 0.8 1 12 5 38 13 0.8 1 11 2 39 6 0.8 1 3 3 40 11 0.8 1 3 8 41 19 0.8 1 15 4 42 18 0.8 1 11 7 43 18 0.8 1 15 3 44 18 0.8 1 14 4 45 7 0.8 1 6 1 46 9 0.8 1 9 47 3 0.8 1 2 1 48 20 0.8 1 11 9 49 13 0.8 1 11 2 50 8 0.8 1 7 1 51 12 0.8 1 6 6 52 17 0.8 1 15 2 53 9 0.8 1 8 1 54 3 0.8 1 1 2 55 29 0.8 1 21 8 56 20 0.8 1 17 3 57 3 0.8 1 3 58 6 0.8 1 4 2 59 11 0.8 1 10 1 60 6 0.8 1 5 1 61 6 0.8 1 4 2 62 15 0.8 1 10 5 63 3 0.8 1 2 1 64 2 0.8 1 1 1 65 3 0.8 1 1 2 66 15 0.8 1 11 4 67 12 0.8 1 11 1 68 4 0.8 1 2 2 69 3 0.8 1 1 2 70 4 0.8 1 4 71 5 0.8 1 4 1 72 8 0.8 1 6 2 73 6 0.8 1 4 2 74 10 0.8 1 7 3 75 5 0.8 1 1 4 76 6 0.8 1 5 1 77 6 0.8 1 5 1 78 7 0.8 1 4 3 79 5 0.8 1 5 80 9 0.8 1 8 1 81 8 0.8 1 5 3 82 6 0.8 1 4 2 83 9 0.8 1 8 1 84 5 0.8 1 3 2 85 8 0.8 1 5 3 86 5 0.8 1 3 2 87 6 0.8 1 5 1 88 8 0.8 1 7 1 89 10 0.8 1 8 2 90 9 0.8 1 9 91 6 0.8 1 6 92 8 0.8 1 6 2 93 2 0.8 1 2 94 8 0.8 1 7 1 95 8 0.8 1 7 1 96 12 0.8 1 10 2 97 9 0.8 1 8 1 98 4 0.8 1 4 99 8 0.8 1 6 2 100 9 0.8 1 7 2 101 3 0.8 1 2 1 102 14 0.8 1 12 2 103 10 0.8 1 7 3 104 11 0.8 1 9 2 105 3 0.8 1 3 106 11 0.8 1 7 4 107 10 0.8 1 6 4 108 6 0.8 1 6 109 7 0.8 1 7 110 8 0.8 1 5 3 111 6 0.8 1 6 112 9 0.8 1 8 1 113 13 0.8 1 9 4 114 17 0.8 1 15 2 115 15 0.8 1 13 2 116 12 0.8 1 11 1 117 9 0.8 1 5 4 118 17 0.8 1 15 2 119 23 0.8 1 18 5 120 11 0.8 1 9 2 121 10 0.8 1 10 122 22 0.8 1 17 5 123 10 0.8 1 10 124 10 0.8 1 9 1 125 14 0.8 1 14 126 19 0.8 1 16 3 127 18 0.8 1 18 128 27 0.8 1 22 5 129 17 0.8 1 15 2 130 19 0.8 1 16 3 131 19 0.8 1 14 5 132 17 0.8 1 17 133 22 0.8 1 20 2 134 26 0.8 1 19 7 135 30 0.8 1 14 16 RUN STATISTICS FOR INPUT FILE: SRR13207050_1.fastq.gz ============================================= 51977624 sequences processed in total