SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308527_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 131605). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308527_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1199.090 s (16.815 µs/read; 3.57 M reads/minute). === Summary === Total reads processed: 71,310,492 Reads with adapters: 34,890,257 (48.9%) Reads written (passing filters): 71,310,492 (100.0%) Total basepairs processed: 10,767,884,292 bp Quality-trimmed: 37,080,414 bp (0.3%) Total written (filtered): 10,307,911,772 bp (95.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 34890257 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.9% C: 3.4% G: 17.9% T: 43.8% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 17485485 17827623.0 0 17485485 2 3232050 4456905.8 0 3232050 3 1452747 1114226.4 0 1452747 4 677764 278556.6 0 677764 5 310436 69639.2 0 310436 6 267720 17409.8 0 267720 7 263708 4352.4 0 263708 8 292207 1088.1 0 292207 9 240966 272.0 0 239577 1389 10 271565 68.0 1 249493 22072 11 281107 17.0 1 252447 28660 12 290755 4.3 1 263436 27319 13 260582 1.1 1 239442 21140 14 256102 1.1 1 230097 26005 15 271133 1.1 1 248119 23014 16 252582 1.1 1 226008 26574 17 284552 1.1 1 252956 31596 18 324862 1.1 1 300822 24040 19 152569 1.1 1 141219 11350 20 240383 1.1 1 221435 18948 21 255471 1.1 1 235067 20404 22 215262 1.1 1 198736 16526 23 253632 1.1 1 233189 20443 24 222740 1.1 1 199000 23740 25 235251 1.1 1 218547 16704 26 191912 1.1 1 179690 12222 27 231166 1.1 1 213092 18074 28 217831 1.1 1 203250 14581 29 211672 1.1 1 192807 18865 30 258062 1.1 1 241982 16080 31 128248 1.1 1 120189 8059 32 203692 1.1 1 190603 13089 33 183772 1.1 1 169441 14331 34 215832 1.1 1 199292 16540 35 195422 1.1 1 185761 9661 36 146756 1.1 1 132151 14605 37 190730 1.1 1 178546 12184 38 144435 1.1 1 136502 7933 39 177176 1.1 1 164523 12653 40 181311 1.1 1 170076 11235 41 146929 1.1 1 136050 10879 42 144420 1.1 1 136381 8039 43 200459 1.1 1 184091 16368 44 107577 1.1 1 98942 8635 45 232583 1.1 1 221113 11470 46 53936 1.1 1 48836 5100 47 125549 1.1 1 118283 7266 48 193016 1.1 1 182772 10244 49 78218 1.1 1 73429 4789 50 128112 1.1 1 119936 8176 51 119879 1.1 1 112234 7645 52 110052 1.1 1 104373 5679 53 98298 1.1 1 93530 4768 54 156911 1.1 1 148489 8422 55 143610 1.1 1 138166 5444 56 41809 1.1 1 38567 3242 57 147911 1.1 1 143028 4883 58 67480 1.1 1 64737 2743 59 53621 1.1 1 50774 2847 60 78185 1.1 1 76077 2108 61 26792 1.1 1 24867 1925 62 88783 1.1 1 84338 4445 63 115076 1.1 1 111913 3163 64 32583 1.1 1 31064 1519 65 33148 1.1 1 31158 1990 66 75266 1.1 1 72101 3165 67 57803 1.1 1 55387 2416 68 65181 1.1 1 62539 2642 69 60729 1.1 1 57769 2960 70 105298 1.1 1 101252 4046 71 9389 1.1 1 8464 925 72 8568 1.1 1 7963 605 73 23228 1.1 1 22257 971 74 32790 1.1 1 31410 1380 75 35411 1.1 1 33858 1553 76 34530 1.1 1 33025 1505 77 33470 1.1 1 32049 1421 78 31759 1.1 1 30387 1372 79 29621 1.1 1 28308 1313 80 28390 1.1 1 27022 1368 81 26378 1.1 1 25194 1184 82 24410 1.1 1 23318 1092 83 22803 1.1 1 21814 989 84 21457 1.1 1 20533 924 85 19585 1.1 1 18738 847 86 18608 1.1 1 17750 858 87 17123 1.1 1 16383 740 88 15747 1.1 1 15075 672 89 14607 1.1 1 13985 622 90 13518 1.1 1 12891 627 91 12566 1.1 1 11992 574 92 11680 1.1 1 11184 496 93 10887 1.1 1 10357 530 94 9977 1.1 1 9471 506 95 9112 1.1 1 8713 399 96 8430 1.1 1 8025 405 97 7601 1.1 1 7247 354 98 7151 1.1 1 6792 359 99 6315 1.1 1 6002 313 100 5886 1.1 1 5575 311 101 5443 1.1 1 5186 257 102 4816 1.1 1 4537 279 103 4592 1.1 1 4359 233 104 4047 1.1 1 3871 176 105 3612 1.1 1 3445 167 106 3357 1.1 1 3187 170 107 3012 1.1 1 2850 162 108 2604 1.1 1 2466 138 109 2393 1.1 1 2231 162 110 2105 1.1 1 1984 121 111 1850 1.1 1 1728 122 112 1743 1.1 1 1631 112 113 1503 1.1 1 1401 102 114 1336 1.1 1 1238 98 115 1153 1.1 1 1059 94 116 963 1.1 1 898 65 117 922 1.1 1 852 70 118 751 1.1 1 695 56 119 679 1.1 1 601 78 120 804 1.1 1 731 73 121 630 1.1 1 571 59 122 451 1.1 1 408 43 123 395 1.1 1 341 54 124 347 1.1 1 313 34 125 273 1.1 1 224 49 126 291 1.1 1 236 55 127 238 1.1 1 192 46 128 186 1.1 1 141 45 129 182 1.1 1 132 50 130 166 1.1 1 129 37 131 165 1.1 1 131 34 132 126 1.1 1 103 23 133 120 1.1 1 102 18 134 120 1.1 1 93 27 135 140 1.1 1 99 41 136 89 1.1 1 66 23 137 83 1.1 1 58 25 138 82 1.1 1 56 26 139 82 1.1 1 67 15 140 71 1.1 1 45 26 141 77 1.1 1 51 26 142 42 1.1 1 27 15 143 68 1.1 1 41 27 144 54 1.1 1 31 23 145 64 1.1 1 29 35 146 58 1.1 1 27 31 147 54 1.1 1 28 26 148 55 1.1 1 29 26 149 76 1.1 1 51 25 150 116 1.1 1 18 98 151 27812 1.1 1 63 27749 RUN STATISTICS FOR INPUT FILE: ERR12308527_1.fastq.gz ============================================= 71310492 sequences processed in total