SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308526_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 134025). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308526_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1981.104 s (17.229 µs/read; 3.48 M reads/minute). === Summary === Total reads processed: 114,987,092 Reads with adapters: 57,604,726 (50.1%) Reads written (passing filters): 114,987,092 (100.0%) Total basepairs processed: 17,363,050,892 bp Quality-trimmed: 38,867,606 bp (0.2%) Total written (filtered): 16,649,249,887 bp (95.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 57604726 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.7% C: 0.5% G: 15.1% T: 47.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 30584330 28746773.0 0 30584330 2 4737935 7186693.2 0 4737935 3 2163988 1796673.3 0 2163988 4 1109584 449168.3 0 1109584 5 425853 112292.1 0 425853 6 365665 28073.0 0 365665 7 358908 7018.3 0 358908 8 399889 1754.6 0 399889 9 369124 438.6 0 367693 1431 10 382548 109.7 1 354601 27947 11 414187 27.4 1 371900 42287 12 430363 6.9 1 391777 38586 13 360017 1.7 1 334324 25693 14 394847 1.7 1 354538 40309 15 396299 1.7 1 366440 29859 16 380895 1.7 1 342144 38751 17 421145 1.7 1 377723 43422 18 486296 1.7 1 452622 33674 19 238009 1.7 1 222215 15794 20 365759 1.7 1 340379 25380 21 395497 1.7 1 365253 30244 22 330210 1.7 1 308549 21661 23 388720 1.7 1 360225 28495 24 356395 1.7 1 321392 35003 25 359280 1.7 1 336396 22884 26 312708 1.7 1 294899 17809 27 365260 1.7 1 339974 25286 28 332843 1.7 1 314777 18066 29 338999 1.7 1 312521 26478 30 398547 1.7 1 376286 22261 31 230630 1.7 1 218606 12024 32 327115 1.7 1 310103 17012 33 296437 1.7 1 277803 18634 34 330470 1.7 1 307233 23237 35 274828 1.7 1 262891 11937 36 316236 1.7 1 295059 21177 37 284942 1.7 1 273050 11892 38 275468 1.7 1 254908 20560 39 268280 1.7 1 255414 12866 40 271543 1.7 1 257091 14452 41 310262 1.7 1 293986 16276 42 264060 1.7 1 249181 14879 43 325460 1.7 1 293647 31813 44 172064 1.7 1 159908 12156 45 429354 1.7 1 408606 20748 46 71644 1.7 1 65340 6304 47 243408 1.7 1 227665 15743 48 1355970 1.7 1 1310941 45029 49 218342 1.7 1 207766 10576 50 206560 1.7 1 197332 9228 51 247853 1.7 1 238740 9113 52 132219 1.7 1 127554 4665 53 68575 1.7 1 65683 2892 54 365425 1.7 1 356497 8928 55 56410 1.7 1 54239 2171 56 23025 1.7 1 21707 1318 57 95588 1.7 1 93265 2323 58 11409 1.7 1 10517 892 59 29500 1.7 1 28251 1249 60 23710 1.7 1 22578 1132 61 10607 1.7 1 9701 906 62 119606 1.7 1 116502 3104 63 77618 1.7 1 75937 1681 64 14169 1.7 1 13488 681 65 21414 1.7 1 20258 1156 66 101741 1.7 1 99155 2586 67 34542 1.7 1 33135 1407 68 82604 1.7 1 79633 2971 69 89617 1.7 1 86217 3400 70 162473 1.7 1 157409 5064 71 39866 1.7 1 37837 2029 72 7454 1.7 1 6807 647 73 18541 1.7 1 17888 653 74 41796 1.7 1 40637 1159 75 55189 1.7 1 53525 1664 76 58332 1.7 1 56530 1802 77 57612 1.7 1 55770 1842 78 54311 1.7 1 52541 1770 79 51034 1.7 1 49404 1630 80 48515 1.7 1 46886 1629 81 45348 1.7 1 43914 1434 82 42662 1.7 1 41316 1346 83 39730 1.7 1 38491 1239 84 36984 1.7 1 35755 1229 85 34397 1.7 1 33322 1075 86 32518 1.7 1 31508 1010 87 29753 1.7 1 28775 978 88 28211 1.7 1 27316 895 89 25819 1.7 1 25007 812 90 23665 1.7 1 22906 759 91 22219 1.7 1 21584 635 92 20304 1.7 1 19681 623 93 18650 1.7 1 18069 581 94 17028 1.7 1 16541 487 95 15899 1.7 1 15413 486 96 14717 1.7 1 14330 387 97 13158 1.7 1 12795 363 98 12000 1.7 1 11612 388 99 11002 1.7 1 10658 344 100 10048 1.7 1 9780 268 101 9118 1.7 1 8830 288 102 8210 1.7 1 7954 256 103 7280 1.7 1 7061 219 104 6542 1.7 1 6340 202 105 5569 1.7 1 5411 158 106 4859 1.7 1 4712 147 107 4408 1.7 1 4277 131 108 3616 1.7 1 3487 129 109 3306 1.7 1 3198 108 110 2822 1.7 1 2743 79 111 2394 1.7 1 2322 72 112 2077 1.7 1 1996 81 113 1768 1.7 1 1698 70 114 1318 1.7 1 1265 53 115 1103 1.7 1 1059 44 116 922 1.7 1 883 39 117 736 1.7 1 695 41 118 560 1.7 1 527 33 119 386 1.7 1 355 31 120 343 1.7 1 320 23 121 292 1.7 1 262 30 122 197 1.7 1 172 25 123 159 1.7 1 132 27 124 107 1.7 1 92 15 125 91 1.7 1 67 24 126 81 1.7 1 56 25 127 55 1.7 1 43 12 128 54 1.7 1 39 15 129 74 1.7 1 58 16 130 40 1.7 1 32 8 131 49 1.7 1 36 13 132 43 1.7 1 26 17 133 44 1.7 1 25 19 134 22 1.7 1 12 10 135 19 1.7 1 13 6 136 28 1.7 1 15 13 137 43 1.7 1 23 20 138 14 1.7 1 6 8 139 39 1.7 1 15 24 140 22 1.7 1 10 12 141 23 1.7 1 7 16 142 18 1.7 1 5 13 143 24 1.7 1 11 13 144 15 1.7 1 6 9 145 23 1.7 1 5 18 146 21 1.7 1 2 19 147 14 1.7 1 3 11 148 32 1.7 1 5 27 149 24 1.7 1 5 19 150 28 1.7 1 4 24 151 3654 1.7 1 10 3644 RUN STATISTICS FOR INPUT FILE: ERR12308526_1.fastq.gz ============================================= 114987092 sequences processed in total