Bismark report for: ERR12308525_val_1.fq.gz and ERR12308525_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 86241924 Number of paired-end alignments with a unique best hit: 30136136 Mapping efficiency: 34.9% Sequence pairs with no alignments under any condition: 46119674 Sequence pairs did not map uniquely: 9986114 Sequence pairs which were discarded because genomic sequence could not be extracted: 16 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 15060889 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 15075231 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1697176669 Total methylated C's in CpG context: 56436562 Total methylated C's in CHG context: 11142854 Total methylated C's in CHH context: 27550209 Total methylated C's in Unknown context: 245339 Total unmethylated C's in CpG context: 192491672 Total unmethylated C's in CHG context: 312564717 Total unmethylated C's in CHH context: 1096990655 Total unmethylated C's in Unknown context: 6790986 C methylated in CpG context: 22.7% C methylated in CHG context: 3.4% C methylated in CHH context: 2.4% C methylated in Unknown context (CN or CHN): 3.5% Bismark completed in 0d 4h 18m 50s