Bismark report for: ERR12308522_val_1.fq.gz and ERR12308522_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 84026603 Number of paired-end alignments with a unique best hit: 21731805 Mapping efficiency: 25.9% Sequence pairs with no alignments under any condition: 51543723 Sequence pairs did not map uniquely: 10751075 Sequence pairs which were discarded because genomic sequence could not be extracted: 5 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10863770 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 10868030 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1109717645 Total methylated C's in CpG context: 25020816 Total methylated C's in CHG context: 7274108 Total methylated C's in CHH context: 21600562 Total methylated C's in Unknown context: 316723 Total unmethylated C's in CpG context: 120647227 Total unmethylated C's in CHG context: 178070272 Total unmethylated C's in CHH context: 757104660 Total unmethylated C's in Unknown context: 6653943 C methylated in CpG context: 17.2% C methylated in CHG context: 3.9% C methylated in CHH context: 2.8% C methylated in Unknown context (CN or CHN): 4.5% Bismark completed in 0d 7h 8m 7s