Bismark report for: ERR12308521_val_1.fq.gz and ERR12308521_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 88809775 Number of paired-end alignments with a unique best hit: 23991269 Mapping efficiency: 27.0% Sequence pairs with no alignments under any condition: 50168303 Sequence pairs did not map uniquely: 14650203 Sequence pairs which were discarded because genomic sequence could not be extracted: 6 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11980710 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 12010553 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1196734714 Total methylated C's in CpG context: 23022181 Total methylated C's in CHG context: 4962470 Total methylated C's in CHH context: 13677423 Total methylated C's in Unknown context: 240736 Total unmethylated C's in CpG context: 134445421 Total unmethylated C's in CHG context: 191010006 Total unmethylated C's in CHH context: 829617213 Total unmethylated C's in Unknown context: 7315652 C methylated in CpG context: 14.6% C methylated in CHG context: 2.5% C methylated in CHH context: 1.6% C methylated in Unknown context (CN or CHN): 3.2% Bismark completed in 0d 5h 12m 49s