SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308517_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 134598). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308517_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1342.691 s (16.313 µs/read; 3.68 M reads/minute). === Summary === Total reads processed: 82,309,934 Reads with adapters: 40,583,037 (49.3%) Reads written (passing filters): 82,309,934 (100.0%) Total basepairs processed: 12,428,800,034 bp Quality-trimmed: 35,713,352 bp (0.3%) Total written (filtered): 11,873,615,732 bp (95.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 40583037 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.3% C: 2.0% G: 17.4% T: 46.2% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 20403383 20577483.5 0 20403383 2 3636495 5144370.9 0 3636495 3 1645328 1286092.7 0 1645328 4 795191 321523.2 0 795191 5 343934 80380.8 0 343934 6 294046 20095.2 0 294046 7 291074 5023.8 0 291074 8 317188 1255.9 0 317188 9 276484 314.0 0 274683 1801 10 297028 78.5 1 272766 24262 11 313996 19.6 1 279870 34126 12 327999 4.9 1 296085 31914 13 281887 1.2 1 258064 23823 14 299740 1.2 1 266712 33028 15 307310 1.2 1 280763 26547 16 281050 1.2 1 249841 31209 17 318217 1.2 1 281962 36255 18 384359 1.2 1 356182 28177 19 147909 1.2 1 135760 12149 20 277633 1.2 1 254476 23157 21 265173 1.2 1 243851 21322 22 241489 1.2 1 221952 19537 23 277063 1.2 1 254235 22828 24 249730 1.2 1 222397 27333 25 261636 1.2 1 242481 19155 26 217447 1.2 1 202941 14506 27 258796 1.2 1 238150 20646 28 242432 1.2 1 225562 16870 29 244891 1.2 1 222814 22077 30 286377 1.2 1 268517 17860 31 140854 1.2 1 131651 9203 32 221244 1.2 1 206638 14606 33 203618 1.2 1 188056 15562 34 211055 1.2 1 196558 14497 35 231482 1.2 1 214939 16543 36 211478 1.2 1 192793 18685 37 164172 1.2 1 156422 7750 38 197666 1.2 1 180819 16847 39 203875 1.2 1 187320 16555 40 205847 1.2 1 186634 19213 41 247992 1.2 1 234464 13528 42 125899 1.2 1 119376 6523 43 177362 1.2 1 162474 14888 44 141895 1.2 1 131425 10470 45 262431 1.2 1 250350 12081 46 56893 1.2 1 51522 5371 47 152474 1.2 1 143493 8981 48 213795 1.2 1 201356 12439 49 106375 1.2 1 100112 6263 50 150073 1.2 1 139853 10220 51 148397 1.2 1 137934 10463 52 134974 1.2 1 127926 7048 53 124161 1.2 1 117698 6463 54 166627 1.2 1 156056 10571 55 180907 1.2 1 173516 7391 56 57361 1.2 1 52938 4423 57 175396 1.2 1 168690 6706 58 115136 1.2 1 110712 4424 59 66604 1.2 1 62663 3941 60 110270 1.2 1 107263 3007 61 45951 1.2 1 43067 2884 62 112948 1.2 1 107152 5796 63 145606 1.2 1 141319 4287 64 58438 1.2 1 56118 2320 65 46655 1.2 1 43845 2810 66 97434 1.2 1 93465 3969 67 87993 1.2 1 84327 3666 68 96109 1.2 1 92032 4077 69 87609 1.2 1 82894 4715 70 150693 1.2 1 144947 5746 71 12335 1.2 1 11154 1181 72 11711 1.2 1 10859 852 73 33916 1.2 1 32464 1452 74 47979 1.2 1 46022 1957 75 51743 1.2 1 49599 2144 76 51876 1.2 1 49643 2233 77 49312 1.2 1 47212 2100 78 47162 1.2 1 45148 2014 79 44940 1.2 1 43045 1895 80 42695 1.2 1 40911 1784 81 40002 1.2 1 38233 1769 82 37960 1.2 1 36372 1588 83 35751 1.2 1 34190 1561 84 33480 1.2 1 32077 1403 85 31864 1.2 1 30568 1296 86 29220 1.2 1 28015 1205 87 27664 1.2 1 26471 1193 88 25418 1.2 1 24394 1024 89 24054 1.2 1 23027 1027 90 22190 1.2 1 21245 945 91 20714 1.2 1 19907 807 92 19109 1.2 1 18329 780 93 17834 1.2 1 17072 762 94 16324 1.2 1 15678 646 95 15309 1.2 1 14696 613 96 13901 1.2 1 13307 594 97 12812 1.2 1 12243 569 98 11782 1.2 1 11290 492 99 10940 1.2 1 10493 447 100 9905 1.2 1 9497 408 101 8998 1.2 1 8650 348 102 8237 1.2 1 7905 332 103 7410 1.2 1 7110 300 104 6659 1.2 1 6391 268 105 6019 1.2 1 5757 262 106 5367 1.2 1 5163 204 107 4797 1.2 1 4596 201 108 4267 1.2 1 4080 187 109 3648 1.2 1 3497 151 110 3379 1.2 1 3220 159 111 2972 1.2 1 2826 146 112 2619 1.2 1 2494 125 113 2298 1.2 1 2171 127 114 1979 1.2 1 1870 109 115 1607 1.2 1 1519 88 116 1426 1.2 1 1360 66 117 1183 1.2 1 1105 78 118 1022 1.2 1 943 79 119 907 1.2 1 851 56 120 903 1.2 1 842 61 121 731 1.2 1 689 42 122 509 1.2 1 471 38 123 391 1.2 1 334 57 124 333 1.2 1 295 38 125 271 1.2 1 228 43 126 220 1.2 1 190 30 127 196 1.2 1 168 28 128 141 1.2 1 110 31 129 136 1.2 1 109 27 130 137 1.2 1 114 23 131 117 1.2 1 92 25 132 89 1.2 1 71 18 133 82 1.2 1 65 17 134 85 1.2 1 66 19 135 92 1.2 1 70 22 136 67 1.2 1 49 18 137 95 1.2 1 71 24 138 56 1.2 1 37 19 139 64 1.2 1 46 18 140 62 1.2 1 41 21 141 69 1.2 1 54 15 142 47 1.2 1 27 20 143 50 1.2 1 35 15 144 57 1.2 1 34 23 145 59 1.2 1 41 18 146 52 1.2 1 30 22 147 62 1.2 1 33 29 148 63 1.2 1 24 39 149 61 1.2 1 31 30 150 126 1.2 1 16 110 151 31884 1.2 1 57 31827 RUN STATISTICS FOR INPUT FILE: ERR12308517_1.fastq.gz ============================================= 82309934 sequences processed in total