Bismark report for: ERR12308516_val_1.fq.gz and ERR12308516_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 65495731 Number of paired-end alignments with a unique best hit: 18388335 Mapping efficiency: 28.1% Sequence pairs with no alignments under any condition: 36352921 Sequence pairs did not map uniquely: 10754475 Sequence pairs which were discarded because genomic sequence could not be extracted: 14 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9176584 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 9211737 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 912110856 Total methylated C's in CpG context: 16359236 Total methylated C's in CHG context: 3079894 Total methylated C's in CHH context: 8082314 Total methylated C's in Unknown context: 132627 Total unmethylated C's in CpG context: 103884201 Total unmethylated C's in CHG context: 147226697 Total unmethylated C's in CHH context: 633478514 Total unmethylated C's in Unknown context: 5078498 C methylated in CpG context: 13.6% C methylated in CHG context: 2.0% C methylated in CHH context: 1.3% C methylated in Unknown context (CN or CHN): 2.5% Bismark completed in 0d 4h 16m 54s