SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308515_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 138362). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308515_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1382.194 s (18.865 µs/read; 3.18 M reads/minute). === Summary === Total reads processed: 73,268,862 Reads with adapters: 36,191,714 (49.4%) Reads written (passing filters): 73,268,862 (100.0%) Total basepairs processed: 11,063,598,162 bp Quality-trimmed: 36,192,817 bp (0.3%) Total written (filtered): 10,561,717,928 bp (95.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 36191714 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.0% C: 3.1% G: 17.5% T: 44.3% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 17811449 18317215.5 0 17811449 2 3287437 4579303.9 0 3287437 3 1483885 1144826.0 0 1483885 4 697928 286206.5 0 697928 5 318837 71551.6 0 318837 6 275706 17887.9 0 275706 7 272000 4472.0 0 272000 8 301087 1118.0 0 301087 9 251246 279.5 0 250040 1206 10 279697 69.9 1 255808 23889 11 292336 17.5 1 260971 31365 12 304197 4.4 1 274631 29566 13 260002 1.1 1 238056 21946 14 275588 1.1 1 245961 29627 15 280045 1.1 1 256047 23998 16 263690 1.1 1 234785 28905 17 295090 1.1 1 261502 33588 18 337626 1.1 1 311363 26263 19 159705 1.1 1 147644 12061 20 249868 1.1 1 229887 19981 21 263803 1.1 1 241809 21994 22 225157 1.1 1 207242 17915 23 265136 1.1 1 243406 21730 24 231233 1.1 1 206078 25155 25 246462 1.1 1 228556 17906 26 198473 1.1 1 185728 12745 27 239149 1.1 1 220196 18953 28 219468 1.1 1 204440 15028 29 229409 1.1 1 209135 20274 30 263257 1.1 1 246024 17233 31 141904 1.1 1 133285 8619 32 213957 1.1 1 199796 14161 33 188064 1.1 1 174694 13370 34 199726 1.1 1 184723 15003 35 192822 1.1 1 179178 13644 36 223123 1.1 1 204996 18127 37 193517 1.1 1 175117 18400 38 236248 1.1 1 222728 13520 39 119833 1.1 1 113870 5963 40 174806 1.1 1 159949 14857 41 145054 1.1 1 136738 8316 42 165966 1.1 1 156643 9323 43 218444 1.1 1 199424 19020 44 112649 1.1 1 104699 7950 45 244591 1.1 1 232575 12016 46 50984 1.1 1 46499 4485 47 138463 1.1 1 130438 8025 48 220149 1.1 1 206779 13370 49 89313 1.1 1 84008 5305 50 141174 1.1 1 131389 9785 51 138213 1.1 1 128921 9292 52 121239 1.1 1 115142 6097 53 106862 1.1 1 101704 5158 54 185702 1.1 1 176064 9638 55 157045 1.1 1 150835 6210 56 42901 1.1 1 39753 3148 57 167006 1.1 1 161489 5517 58 69157 1.1 1 66166 2991 59 67430 1.1 1 64148 3282 60 81519 1.1 1 79174 2345 61 29705 1.1 1 27840 1865 62 101460 1.1 1 96717 4743 63 131666 1.1 1 128272 3394 64 34536 1.1 1 32982 1554 65 36447 1.1 1 34413 2034 66 85700 1.1 1 82448 3252 67 65699 1.1 1 63050 2649 68 75745 1.1 1 72444 3301 69 71849 1.1 1 68324 3525 70 127492 1.1 1 122357 5135 71 12004 1.1 1 10929 1075 72 8825 1.1 1 8212 613 73 25671 1.1 1 24634 1037 74 38135 1.1 1 36567 1568 75 42794 1.1 1 41044 1750 76 42340 1.1 1 40445 1895 77 40511 1.1 1 38667 1844 78 38486 1.1 1 36908 1578 79 36932 1.1 1 35356 1576 80 35041 1.1 1 33537 1504 81 32807 1.1 1 31306 1501 82 30858 1.1 1 29593 1265 83 29148 1.1 1 27907 1241 84 26722 1.1 1 25556 1166 85 25104 1.1 1 24023 1081 86 23756 1.1 1 22778 978 87 22186 1.1 1 21234 952 88 21035 1.1 1 20132 903 89 19315 1.1 1 18504 811 90 18667 1.1 1 17880 787 91 17278 1.1 1 16549 729 92 15855 1.1 1 15207 648 93 14579 1.1 1 13973 606 94 13843 1.1 1 13260 583 95 12727 1.1 1 12208 519 96 11669 1.1 1 11147 522 97 10849 1.1 1 10396 453 98 10207 1.1 1 9777 430 99 9242 1.1 1 8852 390 100 8825 1.1 1 8486 339 101 8036 1.1 1 7640 396 102 7434 1.1 1 7103 331 103 6746 1.1 1 6487 259 104 6251 1.1 1 5988 263 105 5542 1.1 1 5294 248 106 5226 1.1 1 4982 244 107 4639 1.1 1 4432 207 108 4245 1.1 1 4056 189 109 3863 1.1 1 3702 161 110 3392 1.1 1 3233 159 111 2963 1.1 1 2827 136 112 2778 1.1 1 2646 132 113 2298 1.1 1 2179 119 114 2056 1.1 1 1936 120 115 1873 1.1 1 1766 107 116 1573 1.1 1 1478 95 117 1337 1.1 1 1253 84 118 1123 1.1 1 1059 64 119 1033 1.1 1 949 84 120 1095 1.1 1 1037 58 121 921 1.1 1 846 75 122 629 1.1 1 593 36 123 537 1.1 1 490 47 124 407 1.1 1 364 43 125 404 1.1 1 352 52 126 284 1.1 1 248 36 127 261 1.1 1 211 50 128 198 1.1 1 169 29 129 169 1.1 1 140 29 130 152 1.1 1 122 30 131 142 1.1 1 117 25 132 110 1.1 1 88 22 133 134 1.1 1 97 37 134 78 1.1 1 59 19 135 107 1.1 1 80 27 136 82 1.1 1 56 26 137 88 1.1 1 68 20 138 73 1.1 1 56 17 139 83 1.1 1 61 22 140 54 1.1 1 38 16 141 79 1.1 1 59 20 142 71 1.1 1 37 34 143 51 1.1 1 29 22 144 69 1.1 1 37 32 145 73 1.1 1 45 28 146 71 1.1 1 41 30 147 87 1.1 1 46 41 148 50 1.1 1 22 28 149 56 1.1 1 37 19 150 113 1.1 1 27 86 151 30041 1.1 1 75 29966 RUN STATISTICS FOR INPUT FILE: ERR12308515_2.fastq.gz ============================================= 73268862 sequences processed in total